Estimation of the 'ntestinal' age by microbiota analysis

• Project/Area Number: 25670292
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Epidemiology and preventive medicine
• Research Institution: Hirosaki University
• Principal Investigator: NAKAJI Shigeyuki 弘前大学, 医学(系)研究科(研究院), 教授 (10192220)
• Co-Investigator(Kenkyū-buntansha): SAWADA Kaori 弘前大学, 大学院医学研究科, 助教 (50624889) ; TAKAHASHI Ippei 弘前大学, 大学院医学研究科, 准教授 (70400132) ; MATSUZAKA Masashi 弘前大学, 大学院医学研究科, 講師 (70431434) ; SATO Satoshi 弘前大学, 大学院医学研究科, 助手 (00725319)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腸内細菌 / 腸年齢 / 推定式 / Clostridium / Bifidobacterium / Lactobacillales / Prevotella / 一般住民 / Clostridum

Outline of Final Research Achievements

The alterations of gut microbiota by the advance in aging on the residents of Hirosaki, Aomori were investigated by the large-scale survey (about 1,000 adults in Iwaki town in Aomori prefecture). The aging introduced a significant increasing numbers of Lactobacillales and Prevotella and decreasing numbers of bifidobacteria and clostridia. Then, the increasing number of lactobacilli and reduction of clostridia from 50 years old to 60 years old are revealed. A proportion of bifidobacteria to all gut microbiota was significantly indicated in more than 60 years old on the only female. The isolation of Prevotella was increased from 50 to 70 years old. These results in the present study suggested that the gut microbiota on male and female was varied thereabout 60 years olds.

Research Products

• [Journal Article] Thyroid ultrasound findings in a follow-up survey of children from three Japanese prefectures: Aomori, Yamanashi, and Nagasaki (2015)
  • Author(s): Naomi Hayashida, Misa Imaizumi, Hiroki Shimura, Fumihiko Furuya, Noriyuki Okubo, Yasushi Asari, Takeshi Nigawara, Sanae Midorikawa, Kazuhiko Kotani, Shigeyuki Nakaji, Akira Ohtsuru, Takashi Akamizu, Masafumi Kitaoka, Shinichi Suzuki, Nobuyuki Taniguchi, Shunichi Yamashita & Noboru Takamura
  • Journal Title: SCIENTIFIC REPORTS Volume: 5 Issue: 1 Pages: 9046-9046
  • DOI: 10.1038/srep09046
  • NAID: 120006987130
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of the histamine H1 receptor in the regulation of sympathetic nerve activity. (2015)
  • Author(s): Murakami M, Yoshikawa T, Nakamura T, Ohba T, Matsuzaki Y, Sawamura D, Kuwasako K, Yanagisawa T, Ono K, Nakaji S, Yanai K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 458 Issue: 3 Pages: 584-589
  • DOI: 10.1016/j.bbrc.2015.02.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Age and gender differences in the factor structure of the Center for Epidemiological Studies Depression Scale among Japanese working individuals. (2015)
  • Author(s): Sugawara N, Yasui-Furukori N, Takahashi I, Matsuzaka M, Nakaji S.
  • Journal Title: Compr Psychiatry. Volume: 56 Pages: 272-278
  • DOI: 10.1016/j.comppsych.2014.09.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RELATIONSHIP BETWEEN EXHALED HYDROGEN AND HUMAN NEUTROPHIL FUNCTION IN THE JAPANESE GENERAL POPULATION (2014)
  • Author(s): 谷川 涼子, 高橋 一平, 大久保 礼由, 小野 真実, 奥村 俊樹, 石橋 剛士, 竹石 洋介, 中山 真樹, 矢野 智彦, 熊坂 義裕, 中路 重之
  • Journal Title: Hirosaki Med J Volume: 65 Pages: 138-146
  • NAID: 110009838238
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 弘前市の一般住民における加齢が腸内常在菌に及ぼす影響. (2014)
  • Author(s): 甲斐知彦, 高橋一平, 沢田かほり, 大久保礼由, 久田貴義, 辨野義己, 中路重之.
  • Journal Title: アンチ・エイジング医学 Volume: 10 Pages: 95-103
  • NAID: 40020006127
  • Peer Reviewed
• [Journal Article] Decrease of serum level of gastrin in healthy Japanese adults by the change of Helicobacter pylori infection. (2014)
  • Author(s): Shimoyama T, Chinda D, Matsuzaka M, Takahashi I, Nakaji S, Fukuda S.
  • Journal Title: J Gastroenterol Hepatol. Volume: 29 Issue: S4 Pages: 25-28
  • DOI: 10.1111/jgh.12773
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 一般住民におけるメタボリックシンドロームと残存歯数の関係 (2014)
  • Author(s): 狭戸尾 真梨子, 高橋 一平, 大久保 礼由, 関根 陽平, 飯塚 浩史, 徳田 糸代, 有賀 玲子, 長谷部 達也, 渡邉 清誉, 松本 秀彦, 中路 重之
  • Journal Title: 体力・栄養・免疫学雑誌 Volume: 24 Pages: 55-61
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] HbA1cと動脈の硬さの関連に関する研究 岩木健康増進プロジェクトでの横断研究と追跡研究 (2014)
  • Author(s): 有賀 玲子, 高橋 一平, 鈴川 一宏, 関根 陽平, 和田 尚子, 高橋 和幸, 山田 睦雄, 田中 里奈, 松坂 方士, 梅田 孝, 中路 重之
  • Journal Title: 体力・栄養・免疫学 Volume: 24 Pages: 35-43
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 感染症とがん (2014)
  • Author(s): 中路 重之, 佐藤 諭, 秋元 直樹
  • Journal Title: 臨床腫瘍プラクティス (1880-3083)10巻4号 Page460-464(2014.11) Volume: 10 Pages: 460-464
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 検診からわかる整形外科疾患 脊椎疾患 頸髄症健診への取り組みと縦断的な腰部脊柱管狭窄症健診から得られた知見を中心に (2014)
  • Author(s): 和田 簡一郎, 小野 睦, 田中 利弘, 板橋 泰斗, 熊谷 玄太郎, 岩崎 宏貴, 佐々木 英嗣, 横山 徹, 沼沢 拓也, 陳 俊輔, 石橋 恭之, 高橋 一平, 梅田 孝, 中路 重之
  • Journal Title: 臨床整形外科 Volume: 49 Pages: 867-871
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Helicobacter pylori感染が中高年男性の骨密度へ及ぼす影響 (2014)
  • Author(s): 珍田 大輔, 下山 克, 松坂 方士, 速水 史郎, 秋元 直樹, 佐藤 諭, 中路 重之, 福田 眞作
  • Journal Title: 消化と吸収 Volume: 36 Pages: 356-360
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] POSSIBLE RELATIONSHIP BETWEEN PERCENTAGE OF BODY FAT AND LACTOBACILLALES IN GUT NIICROBIOTA: RESULTS FROM A COMMUNITY-BASED STUDY (2014)
  • Author(s): 渡邉 清誉, 高橋 一平, 沢田 かほり, 大久保 礼由, 松坂 方士, 秋元 直樹, 梅田 孝, 中路 重之, 久田 貴由, 辨野 義己
  • Journal Title: 弘前医学 Volume: 65 Pages: 12-20
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cognitive function and number of teeth in a community-dwelling population in Japan. (2013)
  • Author(s): Saito Y, Sugawara N, Yasui-Furukori N, Takahashi I, Nakaji S, Kimura H.
  • Journal Title: Ann Gen Psychiatry. Volume: 24 Issue: 1 Pages: 20-20
  • DOI: 10.1186/1744-859x-12-20
  • Peer Reviewed
• [Journal Article] Change with increasing age in control of the lower limbs during jump-landing in adolescents: a 5-year prospective study (2013)
  • Author(s): Sasaki Shizuka
  • Journal Title: J Orthop Sci Volume: 18 Issue: 5 Pages: 774-81
  • DOI: 10.1007/s00776-013-0420-3
  • Peer Reviewed
• [Journal Article] Influence of obesity on blood pressure and arterial stiffness in the early teens. (2013)
  • Author(s): Kudo U, Takahashi I, Matsuzaka M, Umeda T, Kitagawa N, Kudo H, Chiba Y, Sasaki E, Nishimura M, Nakaji S.
  • Journal Title: Obes Res Clin Pract Volume: 7 Issue: 3 Pages: e211-e217
  • DOI: 10.1016/j.orcp.2011.12.005
  • Peer Reviewed
• [Journal Article] The influence of blood glucose on neutrophil function in individuals without diabetes. (2013)
  • Author(s): Saito Y, Takahashi I, Iwane K, Okubo N, Nishimura M, Matsuzaka M, Wada N, Miwa T, Umeda T, Nakaji S. 2013
  • Journal Title: Luminescence Volume: 28 Issue: 4 Pages: 569-573
  • DOI: 10.1002/bio.2495
  • Peer Reviewed
• [Journal Article] Serum hyaluronan levels increase with the total number of osteoarthritic joints and are strongly associated with the presence of knee and finger osteoarthritis (2013)
  • Author(s): Sasaki Eiji
  • Journal Title: Int Orthop Volume: 37 Issue: 5 Pages: 925-30
  • DOI: 10.1007/s00264-013-1849-x
  • Peer Reviewed
• [Journal Article] Changes in immune functions during a peaking period in male university soccer players. (2013)
  • Author(s): Ueno Y, Umeda T, Takahashi I, Iwane K, Okubo N, Kuroiwa J, Miyazawa M, Osato R, Yoneda K, Nakaji S.
  • Journal Title: Luminescence. Volume: 28 Issue: 4 Pages: 574-581
  • DOI: 10.1002/bio.2496
  • Peer Reviewed
• [Journal Article] Effect of glutamine supplementation on neutrophil function in male judoists. (2013)
  • Author(s): Sasaki E, Umeda T, Takahashi I, Arata K, Yamamoto Y, Tanabe M, Oyamada K, Hashizume E, Nakaji S.
  • Journal Title: Luminescence Volume: 28 Issue: 4 Pages: 442-449
  • DOI: 10.1002/bio.2474
  • Peer Reviewed
• [Journal Article] Evaliation of locomotive disability using loco-check: a cross sectional study in Japanese general population (2013)
  • Author(s): Sasaki Eiji
  • Journal Title: J Orthop Sci Volume: 18 Issue: 1 Pages: 121-9
  • DOI: 10.1007/s00776-012-0329-2
  • Peer Reviewed
• [Journal Article] Influence of a 3-month training program on muscular damage and neutrophil function in male university freshman judoists. (2013)
  • Author(s): Koga T, Umeda T, Kojima A, Tanabe M, Yamamoto Y, Takahashi I, Iwasaki H, Iwane K, Matsuzaka M, Nakaji S.
  • Journal Title: Luminescence. Volume: 28 Issue: 2 Pages: 136-142
  • DOI: 10.1002/bio.2352
  • Peer Reviewed
• [Journal Article] Effects of soccer matches on neutrophil and lymphocyte functions in female university soccer players. (2013)
  • Author(s): Tsubakihara T, Umeda T, Takahashi I, Matsuzaka M, Iwane K, Tanaka M, Matsuda M, Oyamada K, Aruga R, Nakaji S.
  • Journal Title: Luminescence. Volume: 28 Issue: 2 Pages: 129-135
  • DOI: 10.1002/bio.2351
  • Peer Reviewed
• [Journal Article] Changes in neutrophil functions during a 10-month soccer season and their effects on the physical condition of professional Japanese soccer players. (2013)
  • Author(s): Suda Y, Umeda T, Watanebe K, Kuroiwa J, Sasaki E, Tsukamoto T, Takahashi I, Matsuzaka M, Iwane K, Nakaji S.
  • Journal Title: Luminescence. Volume: 28 Issue: 2 Pages: 121-128
  • DOI: 10.1002/bio.2350
  • Peer Reviewed
• [Journal Article] Effects of dehydration on immune functions after a judo practice session. (2013)
  • Author(s): Chishaki T, Umeda T, Takahashi I, Matsuzaka M, Iwane K, Matsumoto H, Ishibashi G, Ueno Y, Kashiwa N, Nakaji S.
  • Journal Title: Luminescence. Volume: 28 Issue: 2 Pages: 114-120
  • DOI: 10.1002/bio.2349
  • Peer Reviewed
• [Journal Article] Sleep problems in family members of long-term care users in the Japanese rural polulation. (2013)
  • Author(s): Terumi Kogawa, Miya Nishimiya, Shizuka Kurauchi, Ippei Takahashi, Noriyuki Okubo, Kaori Sawada, Shigeyuki Nakaji:
  • Journal Title: Med Biol Volume: 157 Pages: 1170-1177
  • Peer Reviewed
• [Journal Article] Thyroid ultrasound findings in children from three Japanese prefectures: Aomori, Yamanashi and Nagasaki. (2013)
  • Author(s): Hayashida N, Imaizumi M, Shimura H, Okubo N, Asari Y, Nigawara T, Midorikawa S, Kotani K, Nakaji S, Ohtsuru A, Akamizu T, Kitaoka M, Suzuki S, Taniguchi N, Yamashita S, Takamura S
  • Journal Title: PloS One Volume: 8巻 Issue: 12 Pages: e83220-e83220
  • DOI: 10.1371/journal.pone.0083220
  • NAID: 120006986696
  • Peer Reviewed
• [Journal Article] Helicobacter pylori infection is associated with a decreased risk of tooth loss in healthy Japanese Men. (2013)
  • Author(s): Shimoyama T, Higuchi H, Matsuzaka M, Chinda D, Nakaji S, Fukuda S.
  • Journal Title: Jpn J Infect Dis. Volume: 66 Pages: 489-492
  • NAID: 130003390326
  • Peer Reviewed
• [Presentation] 弘前市の一般住民を対象とした腸内細菌の加齢変化に関する評価. (2014)
  • Author(s): 1.甲斐知彦，沢田かほり，大久保礼由，徳田糸代，徳安秀正，山本博，小川武志，高橋一平，中路重之.
  • Organizer: 第24回体力・栄養・免疫学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-08-30 – 2014-08-31
• [Presentation] 機能性腸障害罹患者における腸内細菌叢の検討 (2014)
  • Author(s): 2.秋元直樹，赤池あらた，金子美由紀，伊東良，溝口絵里加，矢野智彦，金野潤，椿原徹也，竹石洋介，徳田糸代，田中里奈，高橋一平，中路重之.
  • Organizer: 第24回体力・栄養・免疫学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-08-30 – 2014-08-31
• [Book] Dr中路が語る あおもり県民の健康 (2013)
  • Author(s): 中路重之.
  • Total Pages: 172
  • Publisher: 東奥日報社