| Project/Area Number |
25670341
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHOJO Hideki 山梨大学, 総合研究部, 准教授 (60284626)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 法医学 / 年齢推定 / NUMTS / ミトコンドリアDNA / DNA二本鎖切断 / 非相同末端連結修復 / マイクロホモロジー / DNA挿入 / NUMITS |
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| Outline of Final Research Achievements |
Estimation of human age is one of the most important problems to be solved in forensic sciences. However, DNA-based approaches are quite limited in this field. In this project we challenged to develop a new method for age determination using the analysis of nuclear insertions of mitochondrial sequences (NUMTS). We focused on the mechanism of NUMTS formation, i.e. insertions of mtDNA occur at the sites of DNA double strand breaks (DSBs) on chromosomal DNA (chrDNA) during the non-homologous end joining repair. Because the DSBs can be produced by reactive oxygen species, one of time dependent factors, newly formed NUMTS could be used for age prediction. Thus, we planned to construct cell lines by which we can detect newly appeared NUMTS using recombinant mtDNA carrying GFP gene expressed only inserted into chrDNA. Also, we tried to construct cell lines by which we can introduce DSBs at specific positions using the endonuclease I-SceI. We now go on with the project.
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