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Development of a novel method for forensic age estimation by NUMTS analysis

Research Project

Project/Area Number 25670341
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Legal medicine
Research InstitutionUniversity of Yamanashi

Principal Investigator

MABUCHI Tadashi  山梨大学, 総合研究部, 助教 (80150308)

Co-Investigator(Kenkyū-buntansha) SHOJO Hideki  山梨大学, 総合研究部, 准教授 (60284626)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords法医学 / 年齢推定 / NUMTS / ミトコンドリアDNA / DNA二本鎖切断 / 非相同末端連結修復 / マイクロホモロジー / DNA挿入 / NUMITS
Outline of Final Research Achievements

Estimation of human age is one of the most important problems to be solved in forensic sciences. However, DNA-based approaches are quite limited in this field. In this project we challenged to develop a new method for age determination using the analysis of nuclear insertions of mitochondrial sequences (NUMTS). We focused on the mechanism of NUMTS formation, i.e. insertions of mtDNA occur at the sites of DNA double strand breaks (DSBs) on chromosomal DNA (chrDNA) during the non-homologous end joining repair. Because the DSBs can be produced by reactive oxygen species, one of time dependent factors, newly formed NUMTS could be used for age prediction. Thus, we planned to construct cell lines by which we can detect newly appeared NUMTS using recombinant mtDNA carrying GFP gene expressed only inserted into chrDNA. Also, we tried to construct cell lines by which we can introduce DSBs at specific positions using the endonuclease I-SceI. We now go on with the project.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] 頭部外傷によるシクロオキシゲナーゼの発現変化2015

    • Author(s)
      猩々英紀、馬淵正
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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