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Application of mRNA expression analysis for human blood identification to degenerated samples that were false-negative by immunochromatography

Research Project

Project/Area Number 25670347
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Legal medicine
Research InstitutionFukuoka University

Principal Investigator

KUBO Shin-ichi  福岡大学, 医学部, 教授 (10205122)

Co-Investigator(Kenkyū-buntansha) MATSUSUE Aya  福岡大学, 医学部, 講師 (70309920)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords個人識別 / 血液証明法 / ヒト特異性 / 血液特異性 / mRNA / glycophorin遺伝子 / beta-spectrin遺伝子 / beta-hemoglobin遺伝子 / gycophorin A遺伝子 / Glycophirin A遺伝子 / β-Specrin遺伝子 / β-Hemoglobin遺伝子
Outline of Final Research Achievements

We often encounter cases in which methamphetamine (MA) mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. We investigated the application of mRNA expression analysis for those samples that showed a false-negative with immunochromatography as an alternative approach that does not depend on the antigen-antibody reaction. We used real-time PCR to examine the expression levels of blood markers such as glycophorin A (GYPA), beta-spectrin (SPTB) and beta-hemoglobin (HBB). HBB was the only marker detected in blood specifically, and GYPA was useful in determining human specificity. HBB showed good detection sensitivity, and was detectable from older blood stains. HBB was exclusively detectable in MA-mixed blood stains. Detergents and disinfectants did not significantly influence the mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2014 2013

All Presentation (2 results)

  • [Presentation] Evaluation of an mRNA expression analysis method to identify human blood in samples not amenable to immunochromatography.2014

    • Author(s)
      S Matsumura, A Matsusue, B Waters, M Kashiwagi, K Hara, S Kubo
    • Organizer
      9th International Symposium on Advances in Legal Medicine
    • Place of Presentation
      Fukuoka
    • Year and Date
      2014-06-16 – 2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] mRNAを指標とした新たなヒト血痕証明法の検討2013

    • Author(s)
      松村秀策,松末綾,久保真一
    • Organizer
      第97次日本法医学会学術全国集会
    • Place of Presentation
      ロイトン札幌
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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