Metabolomic analysis for improvement of healthy lifespan

• Project/Area Number: 25670350
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: Kyoto University
• Principal Investigator: Hiroshi Kondoh 京都大学, 医学(系)研究科(研究院), 助教 (80402890)
• Co-Investigator(Kenkyū-buntansha): 横出 正之 京都大学, 医学(系)研究科(研究院), 教授 (20252447)
• Co-Investigator(Renkei-kenkyūsha): Yanagida Mitsuhiro 沖縄科学技術大学大学院, 教授 (80025428)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 老化

Outline of Final Research Achievements

Metabolic analysis is utilized to detect metabolite profile quantatively and qualitatively, by using LC-MS. BY applying these techniques, We identified glucnoid forms of Reveratorol in skin absorbed condition （Plos One 2014) . Moreover we found the significant overlapping of metabolites between human blood and fission yeast（Mol Biosys 2014). Thus metabolmic approach is highly valuable tool to develop future diagnostic marker or therapuetic targets.

Research Products

• [Journal Article] The Warburg-like regulation of PGAM in tumor. (2015)
  • Author(s): Taisuke Izumi, Takumi Mikawa, Hiroshi Kondoh, Matilde E LLeonart and Akifumi Takaori-Kondo.
  • Journal Title: Trends in Cancer Research Volume: １０ Pages: 103-108
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead. (2015)
  • Author(s): Goodson WH 3rd, 他合計173名。Hiroshi Kondohは59番目
  • Journal Title: Carcinogenesis Volume: ３６ Issue: Suppl 1 Pages: S254-S296
  • DOI: 10.1093/carcin/bgv039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Disruptive chemicals, senescence and immortality. (2015)
  • Author(s): Amancio Carnero, Carmen Blanco-Aparicio , Hiroshi Kondoh , Matilde Lleonart , Juan Fernando Martinez-Leal , Chiara Mondello , A. Ivana Scovassi , John Pierce Wise Sr., Sandra S. Wise and Hemad Yasaey.
  • Journal Title: Carcinogenesis Volume: ３６ Issue: Suppl 1 Pages: S19-S37
  • DOI: 10.1093/carcin/bgv029
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dysregulated glycolysis as an oncogenic event. (2015)
  • Author(s): Takumi Mikawa, Matilde E LLeonart, Akifumi Takaori-Kondo, Nobuya Inagaki, Masayuki Yokode, and Hiroshi Kondoh
  • Journal Title: Cell. Mol. Life Sci. Volume: ７２ Issue: 10 Pages: 1881-92
  • DOI: 10.1007/s00018-015-1840-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Unexpected similarities between the Schizosaccharomyces and human blood metabolomes, and novel human metabolites (2014)
  • Author(s): Romanas Chaleckis, Masahiro Ebe, Tomáš Pluskal, Itsuo Murakami, Hiroshi Kondoh, and Mitsuhiro Yanagida
  • Journal Title: Molecular BioSystems Volume: 10 Issue: 10 Pages: 2538-51
  • DOI: 10.1039/c4mb00346b
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Metabolism of skin-absorbed resveratrol into its glucuronized form in mouse skin. (2014)
  • Author(s): Itsuo Murakami, Romanas Chaleckis, Tomáš Pluskal, Ken Ito, Kousuke Hori, Masahiro Ebe, Mitsuhiro Yanagida and Hiroshi Kondoh.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e115359-e115359
  • DOI: 10.1371/journal.pone.0115359
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Senescence-inducing stress promotes proteolysis of glycolytic enzyme phosphoglycerate mutase via ubiquitin ligase Mdm2 (2014)
  • Author(s): T Mikawa, T Maruyama, K Okamoto, H Nakagama, ME LLeonart, T Tsusaka, K Hori, I Murakami, T Izumi, A T-Kondo, M Yokode, G Peters, D Beach, & H Kondoh
  • Journal Title: Journal of Cell Biology Volume: 204(5) Issue: 5 Pages: 729-45
  • DOI: 10.1083/jcb.201306149
  • Peer Reviewed
• [Journal Article] Hepatocyte nuclear factor-1β promotes glucose uptake and glycolytic activity in ovarian clear carcinoma (2013)
  • Author(s): T Okamoto, M Mandai, N Matsumura, K Yamaguchi, H Kondoh, Y Amano, T Baba, J Hamanishi, K Abiko, K Kosaka, S K, Murphy, S Mori, I Konishi
  • Journal Title: Molecular carcinogenesis Volume: Sep 17 Issue: 1 Pages: 1-15
  • DOI: 10.1002/mc.22072
  • Peer Reviewed
• [Journal Article] miR-125b act as a tumor suppressor in breast tumorigenesis via its novel direct targets ENPEP, CK2-<alpha>, CCNJ, and MEGF9 (2013)
  • Author(s): A Feliciano; J Castellvi; A Artero-Castro; J A Leal; C Romagosa; J Hernández-Losa; V Peg; A Fabra; F Vidal; H Kondoh; S Ramón y Cajal; M E. Lleonart
  • Journal Title: PLoS One Volume: 8(10) Issue: 10 Pages: e76247-e76247
  • DOI: 10.1371/journal.pone.0076247
  • Peer Reviewed
• [Journal Article] Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice (2013)
  • Author(s): Okuda, J., Niizuma, S., Shioi, T., Kato, T., Inuzuka, Y., Kawashima, T., Tamaki, Y., Kawamoto, A., Tanada, Y., Iwanaga, Y., Narazaki, M., Matsuda, T., Adachi, S., Soga, T., Takemura, G., Kondoh, H., Kita, T., Kimura, T.
  • Journal Title: PLoS ONE Volume: 8 Issue: 8 Pages: e72173-e72173
  • DOI: 10.1371/journal.pone.0072173
  • Peer Reviewed
• [Presentation] 老化の多様性を規定する代謝特性～進化におけるトレードオフ (2015)
  • Author(s): 近藤祥司
  • Organizer: 第6回 癌・炎症と抗酸化研究会
  • Place of Presentation: 大分
  • Year and Date: 2015-11-14
• [Presentation] ストレス老化シグナルに拮抗するワールブルグ解糖系転写後制御 (2015)
  • Author(s): 近藤祥司
  • Organizer: 第36回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-23
  • Invited
• [Presentation] メタボロームアプローチによるヒト血液メタボライト解析 (2015)
  • Author(s): 近藤祥司
  • Organizer: 基礎老化学会
  • Place of Presentation: 横浜
  • Year and Date: 2015-06-13
• [Presentation] 老化はなぜ進むのか (2015)
  • Author(s): 近藤祥司
  • Organizer: 第29回日本医学会総会
  • Place of Presentation: 京都
  • Year and Date: 2015-04-05
  • Invited
• [Presentation] Metabolomic approach for human blood (2014)
  • Author(s): 近藤祥司
  • Organizer: 日本基礎老化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-06-26 – 2014-06-27
• [Presentation] 高齢者の多様性を反映する代謝マーカーの血液メタボローム探索 (2014)
  • Author(s): 近藤祥司
  • Organizer: 第14回日本抗加齢医学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-06-06 – 2014-06-08
• [Presentation] ストレス老化シグナルによる解糖系調節の誘導する生体防御バリアー形成
  • Author(s): 近藤祥司
  • Organizer: 生化学若手の会
  • Place of Presentation: 大阪大学
• [Presentation] ストレス老化シグナルによる解糖系調節の誘導する癌化バリアー形成
  • Author(s): 近藤祥司
  • Organizer: 第一回癌と代謝研究会
  • Place of Presentation: 慶応大学山形キャンパス
• [Presentation] マウスにおけるレスベラトロールの経皮吸収と代謝
  • Author(s): 村上逸雄、Romanas Chaleckis、伊藤健、近藤祥司
  • Organizer: 老年医学会近畿地方会
  • Place of Presentation: 京都大学
• [Book] シリーズ進化生物学の新潮流；老化という生存戦略；進化におけるトレードオフ (2015)
  • Author(s): 近藤祥司
  • Total Pages: 222
  • Publisher: 日本評論社
• [Book] 老化生物学 老いと寿命のメカニズム (2015)
  • Author(s): 近藤祥司
  • Total Pages: 390
  • Publisher: MEDSI
• [Remarks] 近藤祥司ラボホームページ
  • URL: http://www.anti-aging.jpn.com/
• [Remarks] http://www.anti-aging.jpn.com/research/