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The development of the disease model using humanized intestine in mice.

Research Project

Project/Area Number 25670367
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

WATANABE Mamoru  東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (10175127)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Tetsuya  東京医科歯科大学, 医歯学総合研究科, 教授 (70265809)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsヒト化マウス / 大腸移植 / 移植モデル / 発癌モデル
Outline of Final Research Achievements

The establishment of the animal model that reflects the pathogenesis and the prediction to the therapy for the intestinal bowel disease and colon cancer has been required. In this study, we therefore aimed to establish the mice model, which has humanized intestine by developing our original technic “the method for the plantation of the primary intestinal epithelial cells to the colon”.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Invited: 12 results)

  • [Journal Article] Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon2014

    • Author(s)
      Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T
    • Journal Title

      Genes & Development

      Volume: 28(16) Issue: 16 Pages: 1752-1757

    • DOI

      10.1101/gad.245233.114

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of Magnetic Resonance and Balloon Enteroscopic Examination of Deep Small Intestine in Patients with Crohn's Disease.2014

    • Author(s)
      Takenaka K, Ohtsuka K,Watanabe M et al
    • Journal Title

      Gastroenterology

      Volume: [Epub ahead of print] Issue: 2 Pages: 334-342

    • DOI

      10.1053/j.gastro.2014.04.008

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluorescent labelling of intestinal epithelial cells reveals independent long-lived intestinal stem cells in a crypt.2014

    • Author(s)
      N. Horita, K. Tsuchiya, R. Hayashi, K. Fukushima, S. Hibiya, M. Fukuda, Y. Kano, T. Mizutani, Y. Nemoto, S. Yui, R. Okamoto, T. Nakamura, M. Watanabe
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 454 Issue: 4 Pages: 493-499

    • DOI

      10.1016/j.bbrc.2014.10.091

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hes1 promotes the IL-22-mediated antimicrobial response by enhancing STAT3-dependent transcription in human intestinal epithelial cells2014

    • Author(s)
      Murano T, Okamoto R, Ito G, Nakata T, Hibiya S, Shimizu H, Fujii S, Kano Y, Mizutani T, Yui S, Akiyama-Morio J, Nemoto Y, Tsuchiya K, Nakamura T, Watanabe M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 443 Issue: 3 Pages: 840-846

    • DOI

      10.1016/j.bbrc.2013.12.061

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis.2014

    • Author(s)
      Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 2 Pages: e88369-e88369

    • DOI

      10.1371/journal.pone.0088369

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury.2013

    • Author(s)
      Fordham RP, Yui S, Nakamura T, Watanabe M et. al
    • Journal Title

      Cell Stem Cell

      Volume: 13 Issue: 6 Pages: 734-744

    • DOI

      10.1016/j.stem.2013.09.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lineage-specific expression of Bestrophin-2 and Bestrophin-4 in human intestinal epithelial cells.2013

    • Author(s)
      Ito G, Okamoto R, Nakamura T, Watanabe M et al
    • Journal Title

      PloS One.

      Volume: 8 Issue: 11 Pages: e79693-e79693

    • DOI

      10.1371/journal.pone.0079693

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Th1/Th17-Mediated Interstitial Pneumonia in Chronic Colitis Mice Independent of Intestinal Microbiota.2013

    • Author(s)
      Nemoto Y, Watanabe M et al
    • Journal Title

      J Immunol.

      Volume: 190 Issue: 12 Pages: 6616-6625

    • DOI

      10.4049/jimmunol.1202930

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bone marrow-mesenchymal stem cells are a major source of interleukin-7 and sustain colitis by forming the niche for colitogenic CD4+ memory T cells.2013

    • Author(s)
      Nemoto Y, Nakamura T, Watanabe M et al
    • Journal Title

      Gut.

      Volume: 62 Issue: 8 Pages: 1142-1152

    • DOI

      10.1136/gutjnl-2012-302029

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Molecular mechanism of inflammation–associated colorectal cancer.2014

    • Author(s)
      Watanabe M
    • Organizer
      APDW2014
    • Place of Presentation
      Indonesia(Bali)
    • Year and Date
      2014-11-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Challenges of IBD in Asia – Stem cell therapy.2014

    • Author(s)
      Watanabe M
    • Organizer
      APDW2014
    • Place of Presentation
      Indonesia(Bali)
    • Year and Date
      2014-11-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Tacrolimus and infliximab in patients with refractory ulcerative colitis.2014

    • Author(s)
      Watanabe M
    • Organizer
      TSIBD2014
    • Place of Presentation
      Taiwan(Taipei)
    • Year and Date
      2014-11-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Stem cell transplantation in IBD, are we there yet?2014

    • Author(s)
      Watanabe M
    • Organizer
      TSIBD2014
    • Place of Presentation
      Taiwan (Kaohsiung)
    • Year and Date
      2014-11-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Stem cell therapy: What, When, and How?2014

    • Author(s)
      Watanabe M
    • Organizer
      SIDDS2014
    • Place of Presentation
      Korea(Seoul)
    • Year and Date
      2014-10-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Gut as a second brain to regulate human whole body?2014

    • Author(s)
      Watanabe M
    • Organizer
      Cluster Lectures,
    • Place of Presentation
      Germany(Hamburg, Borstel & Kiel)
    • Year and Date
      2014-10-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Stem cell as a promising target for IBD: From the bench to the bedside.2014

    • Author(s)
      Watanabe M
    • Organizer
      AOCC2014
    • Place of Presentation
      Korea(Seoul)
    • Year and Date
      2014-06-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] JGF Marshal & Warren Lecture: Adult tissue stem cell therapy for gastrointestinal diseases.

    • Author(s)
      Watanabe M
    • Organizer
      GASTRO 2013.
    • Place of Presentation
      Shanghai, China
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Colonic Stem Cell Culture and Transplantation-Application in Mucosal Immunology.

    • Author(s)
      Watanabe M
    • Organizer
      ICMI 2013
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Colonic Stem Cell Culture and Transplantation -Application in Mucosal Immunology-.

    • Author(s)
      Watanabe M
    • Organizer
      RCAI-JSI International Symposium.
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Epidemiology of Asian IBD.

    • Author(s)
      Watanabe M
    • Organizer
      1st Annual Meeting of AOCC.
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] State-of-the-Art lecture: Stem cell therapy in IBD?

    • Author(s)
      Watanabe M
    • Organizer
      Falk Sympo 2013
    • Place of Presentation
      Stuttgart, Germany
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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