Identification of SNPs on mature micro RNA to analyze origin of miRNA
Project/Area Number |
25670369
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Hiroshima University |
Principal Investigator |
CHAYAMA Kazuaki 広島大学, 医歯薬保健学研究院, 教授 (00211376)
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Project Period (FY) |
2013-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
Keywords | 肝臓学 / SNP / digital PCR / microRNA / transplantation |
Research Abstract |
Recent research made it clear that there are certain amount of circulating miRNA in serum, which present in serum associated with proteins such as AGO1 and AGO2. Such AGO-protein bound miRNA is quite stable and detectable by polymerase chain reaction after extraction from the serum samples and reverse transcription. We attempted to analyze organs which secrete each miRNA by analyzing circulating miRNA that is present in serum samples. To perform this analysis we tried to identify SNPs that is present in miRNA. We searched for such SNP in entire human genome analyzing human genome database and identified several candidate SNPs. Although we attempted to detect such miRNA with digital PCR, detection of small amount of miRNA was quite difficult. Further study is needed to detect such small amount of miRNA in serum samples.
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Report
(2 results)
Research Products
(89 results)
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[Journal Article] Daclatasvir plus asunaprevir for chronic HCV genotype 1b infection2014
Author(s)
Kumada H, Suzuki Y, Ikeda K, Toyota J, Karino Y, Chayama K, Kawakami Y, Ido A, Yamamoto K, Takaguchi K, Izumi N, Koike K, Takehara T, Kawada N, Sata M, Miyagoshi H, Eley T, McPhee F, Damokosh A, Ishikawa H, and Hughes E
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Journal Title
Hepatology
Volume: (in press)
Issue: 6
Pages: 2083-2091
DOI
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Peer Reviewed / Open Access
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[Journal Article] A randomized trial of daclatasvir with peginterferon alfa-2b and ribavirin for HCV genotype 1 Infection.2014
Author(s)
Suzuki F, Toyoa J, Ikeda K, Chayama K, Mochida S, Hayashi N, Ishikawa H, Miyagoshi H, Hu W, McPhee F, Hughes
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Journal Title
Antiviral Ther
Volume: in press
Issue: 5
Pages: 491-499
DOI
Related Report
Peer Reviewed
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[Journal Article] Targeted next-generation sequencing and fine linkage disequilibrium mapping reveals association of PNPLA3 and PARVB with the severity of nonalcoholic fatty liver disease2014
Author(s)
Kitamoto T, Kitamoto A, Yoneda M, Hyogo H, Ochi H, Mizusawa S, Ueno T, Nakao K, Sekine A, Chayama K, Nakajima A, Hotta K
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Journal Title
Journal of Human Genetics
Volume: 6
Issue: 5
Pages: 241-6
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Interleukin 28B polymorphism predicts interferon plus ribavirin treatment outcome in patients with hepatitis C virus-related liver cirrhosis: A multicenter retrospective study in Japan.2014
Author(s)
Shakado S, Sakisaka S, Okanoue T, Chayama K, Izumi N, Toyoda J, Tanaka E, Ido A, Takehara T, Yoshioka K, Hiasa Y, Nomura H, Seike M, Ueno Y, Kumada H.
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Journal Title
Hepatol Res
Volume: 44
Issue: 9
Pages: 983-992
DOI
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Peer Reviewed / Open Access
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[Journal Article] Combination therapies with NS5A, NS3 and NS5B inhibitors on different genotypes of hepatitis C virus in human hepatocyte chimeric mice2013
Author(s)
Shi, N. Hiraga, N. Imamura, M. Hayes, C. N. Zhang, Y. Kosaka, K. Okazaki, A. Murakami, E. Tsuge, M. Abe, H. Aikata, H. Takahashi, S. Ochi, H. Tateno-Mukaidani, C. Yoshizato, K. Matsui, H. Kanai, A. Inaba, T. McPhee, F. Gao, M. Chayama, K.
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Journal Title
Gut
Volume: 62
Issue: 7
Pages: 1055-1061
DOI
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Peer Reviewed
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[Journal Article] HLA-DQB1*03 Confers Susceptibility to Chronic Hepatitis C in Japanese: A Genome-Wide Association Study.2013
Author(s)
Miki D, Ochi H, Takahashi A, Hayes CN, Urabe Y, Abe H, Kawaoka T, Tsuge M, Hiraga N, Imamura M, Kawakami Y, Aikata H, Takahashi S, Akuta N, Suzuki F, Ikeda K, Kumada H, Karino Y, Toyota J, Tsunoda T, Kubo M, Kamatani N, Nakamura Y, Chayama K.
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Journal Title
PLoS One.
Volume: 8
Issue: 12
Pages: e84226-e84226
DOI
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Peer Reviewed
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[Journal Article] Autologous Bone-Marrow Mesenchymal Stem Cell Implantation and Endothelial Function in a Rabbit Ischemic Limb Model2013
Author(s)
Mikami S, Nakashima A, Nakagawa K, Maruhashi T, Iwamoto Y, Kajikawa M, Matsumoto T, Kihara Y, Chayama K, Noma K, Ochi M, Nishimura M, Tsuji K, Kato Y, Goto C, Higashi Y
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Journal Title
PLOS ONE
Volume: 8(7)
Issue: 7
Pages: e67739-e67739
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Identification of a Functional Variant in the MICA Promoter Which Regulates MICA Expression and Increases HCV-Related Hepatocellular Carcinoma Risk.2013
Author(s)
Lo PH, Urabe Y, Kumar V, Tanikawa C, Koike K, Kato N, Miki D, Chayama K, Kubo M, Nakamura Y, Matsuda K.
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Journal Title
PLoS One.
Volume: 8
Issue: 4
Pages: e61279-e61279
DOI
Related Report
Peer Reviewed
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[Journal Article] Genetic analyses reveal a role for vitamin D insufficiency in HCV-associated hepatocellular carcinoma development2013
Author(s)
Lange C M, Miki D, Ochi H, Nischalke H D, Bojunga J, Bibert S, Morikawa K, Gouttenoire J, Cerny A, Dufour J F, Gorgievski-Hrisoho M, Heim M H, Malinverni R, Mullhaupt B, Negro F, Semela D, Kutalik Z, Muller T, Spengler U, Berg T, Chayama K, Moradpour D, Bochud P Y, Hiroshima Liver Study G, and Swiss Hepatitis C C S G
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Journal Title
PLoS One
Volume: 8
Issue: 5
Pages: e64053-e64053
DOI
Related Report
Peer Reviewed
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[Journal Article] Circulating microRNA-22 correlates with microRNA-122 and represents viral replication and liver injury in patients with chronic hepatitis B.2013
Author(s)
Arataki K, Hayes CN, Akamatsu S, Akiyama R, Abe H, Tsuge M, Miki D, Ochi H, Hiraga N, Imamura M, Takahashi S, Aikata H, Kawaoka T, Kawakami H, Ohishi W, Chayama K.
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Journal Title
Journal of Medical Virology
Volume: 85(5)
Issue: 5
Pages: 789-98
DOI
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Peer Reviewed
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[Book] 肝臓、ゲノタイプ3a型のC型急性肝炎の1例―IL28B遺伝子からの考察―2013
Author(s)
小林知樹,川上由育,平松憲,瀧川英彦,野中裕広,田中未央,苗代典昭,中原隆志,本田洋士,宮木大輔,長沖祐子,河岡友和,高木慎太郎,柘植雅貴,今村道雄,兵庫秀幸,相方浩,高橋祥一,茶山一彰
Publisher
一般社団法人日本肝臓学会
Related Report
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[Book] 肝臓2013
Author(s)
小林知樹, 川上由育, 平松 憲, 瀧川英彦, 野中裕広, 田中未央, 苗代典昭, 中原隆志, 本田洋士, 宮木大輔, 長沖祐子, 河岡友和, 高木慎太郎, 柘植雅貴, 今村道雄, 兵庫秀幸, 相方 浩, 高橋祥一, 茶山一彰
Total Pages
7
Publisher
一般社団法人日本肝臓学会
Related Report
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[Book] 消化器内科2013
Author(s)
今村道雄, 川上由育, 茶山一彰
Total Pages
4
Publisher
科学評論社
Related Report
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