| Project/Area Number |
25670376
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
YASUNAGA Masahiro 独立行政法人国立がん研究センター, その他部局等, ユニット長 (80450576)
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| Research Collaborator |
MATSUMURA Yasuhiro 国立がん研究センター、臨床開発センター, 新薬開発分野, 分野長 (90209619)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 大腸がん / SLC6A6 / ５－ＦＵ / 抗がん剤耐性 / がん幹細胞 / 造血幹細胞 / トランスポーター / バイオインフォマティクス / 幹細胞 / 5-FU耐性 |
|---|
| Outline of Final Research Achievements |
SLC6A6-knock down colon cancer (CC) cells showed increased chemosensitivity 5-100 fold. Moreover, in the cells, many genes specific to hematopoietic stem cell (HSC) were decreased. Comprehensive analysis to find the regulatory molecules of the chemoresistance common between HSCs and CC cells was conducted. 1.ATG-gene, 2. FOX-related transcription factor, 3. Integrin-Src or 4. regulator of glucose metabolism were selected. The functional analysis indicated that ATG-gene was strongly associated with the survival of the side population cells and the chemosensitivity in colon cancer cells. We found that it was novel autophagic signaling pathway.
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