Development of local microRNA delivery system and in vivo application

• Project/Area Number: 25670383
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kyoto University
• Principal Investigator: ONO KOH 京都大学, 医学(系)研究科(研究院), 准教授 (00359275)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マイクロRNA / ステント / ナノ粒子 / 動脈硬化 / 冠動脈 / 血管内皮細胞 / 最狭窄 / 炎症 / 血管内皮 / neoaterosclerosis

Outline of Final Research Achievements

Recently, drug eluting stent (DES) is mainly used for the treatment of coronary artery diseases. However, the drug can inhibit the growth of endothelial cells, which causes thrombosis or chronic inflammation. Therefore, the ideal treatment is to regenerate the endothelial cells and inhibit the growth of smooth muscle cells. We utilized miR-126 which can induce the regeneration of endothelial cells and inhibit inflammation for this experiment. We generated cholesterol conjugated double strand synthetic RNAs which can overexpress mature miR-126 locally without any vectors. We loaded there double strand RNAs into poly lactic-co-glycolic acid (PLGA) nano-particles and coated the stent with these PLGA nano-particles. We evaluated the effect of this stent in rabbit model of vascular injury. miR-126-eluting stent could significantly inhibit the restenosis of iliac artery after vascular injury compared with control RNA-eluting stent.

Research Products

• [Journal Article] MicroRNA-33a/b in Lipid Metabolism (2015)
  • Author(s): Ono K, Horie T, Nishino T, Baba O, Kuwabara Y, Yokode M, Kita T, Kimura T.
  • Journal Title: Circulation Journal Volume: 79 Issue: 2 Pages: 278-284
  • DOI: 10.1253/circj.CJ-14-1252
  • NAID: 130004927073
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Impact of Left Ventricular Outflow Tract Obstruction in Takotsubo Cardiomyopathy (2015)
  • Author(s): Kawaji T, Shiomi H, Morimoto T, Tazaki J, Imai M, Saito N, Makiyama T, Shizuta S, Ono K, Kimura T.
  • Journal Title: Circulation Journal Volume: 79 Issue: 4 Pages: 839-846
  • DOI: 10.1253/circj.CJ-14-1148
  • NAID: 130005130261
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA-451 exacerbates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy in mice through suppression of the LKB1/AMPK pathway. (2015)
  • Author(s): Kuwabara Y, Horie T, Baba O, Watanabe S, Nishiga M, Usami S, Izuhara M, Nakao T, Nishino T, Otsu K, Kita T, Kimura T, Ono K.
  • Journal Title: Circulation Research Volume: 116 Issue: 2 Pages: 279-288
  • DOI: 10.1161/circresaha.116.304707
  • Peer Reviewed
• [Journal Article] Effect of Statin Therapy on Cardiovascular Outcomes after Coronary Revascularization in Patients over 80 Years of Age (2014)
  • Author(s): Natsuaki M, Morimoto T, Furukawa Y, Shiomi H, Ono K, Kimura T
  • Journal Title: Atherosclerosis Volume: 237 Issue: 2 Pages: 821-828
  • DOI: 10.1016/j.atherosclerosis.2014.10.108
  • Peer Reviewed / Open Access
• [Journal Article] Cardiac-Specific Inhibition of Kinase Activity in Calcium/Calmodulin-Dependent Protein Kinase Kinase-β Leads to Accelerated Left Ventricular Remodeling and Heart Failure after Transverse Aortic Constriction in Mice (2014)
  • Author(s): Shin Watanabe, Takahiro Horie, Kazuya Nagao, Yasuhide Kuwabara, Osamu Baba, Hitoo Nishi, Naoya Sowa, Michiko Narazaki, Tetsuya Matsuda, Genzou Takemura, Hiromichi Wada, Koji Hasegawa, Takeshi Kimura, Koh Ono
  • Journal Title: PLoS ONE Volume: 9 Issue: 9 Pages: e108201-e108201
  • DOI: 10.1371/journal.pone.0108201
  • NAID: 120005496630
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Pre-infarction Angina Pectoris on Long-term Survival in Patients with ST-segment Elevation Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention (2014)
  • Author(s): Taniguchi T, Shiomi H, Toyota T, Morimoto T, Akao M, Nakatsuma K, Ono K, Makiyama T, Shizuta S, Furukawa Y, Nakagawa Y, Ando K, Kadota K, Horie M, Kimura T.
  • Journal Title: Am J Cardiol Volume: 114 Issue: 8 Pages: 1179-1186
  • DOI: 10.1016/j.amjcard.2014.07.038
  • Peer Reviewed / Open Access
• [Journal Article] Incidence and Outcome of Surgical Procedures After Coronary Artery Bypass Grafting Compared With Those After Percutaneous Coronary Intervention (2014)
  • Author(s): Tokushige A, Shiomi H, Morimoto T, Ono K, Furukawa Y, Nakagawa Y, Kadota K, Ando K, Shizuta S, Tada T, Tazaki J, Kato Y, Hayano M, Abe M, Hamasaki S, Ohishi M, Nakashima H et al.
  • Journal Title: Circ Cardiovasc Interv. Volume: 7 Issue: 4 Pages: 482-491
  • DOI: 10.1161/circinterventions.113.001056
  • Peer Reviewed / Open Access
• [Journal Article] Anticoagulant and Antiplatelet Therapy in Patients with Atrial Fibrillation Undergoing Percutaneous Coronary Intervention (2014)
  • Author(s): Goto K, Nakai K, Shizuta S, Morimoto T, Shiomi H, Natsuaki M, Tahata M, Ota C, Ono K, Makiyama T, Nakagawa Y, Furukawa Y, et al.
  • Journal Title: Am J Cardiol Volume: 114 Issue: 1 Pages: 70-78
  • DOI: 10.1016/j.amjcard.2014.03.060
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Nakazeki F, Ide Y, Koyama S, Sowa N, Yahagi N, Shimano H, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, Ono K.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 5312-5312
  • DOI: 10.1038/srep05312
  • Peer Reviewed / Open Access
• [Journal Article] Long-Term Efficacy and SafetyOutcomes After Unrestricted Use of Drug-Eluting Stents in Patients With AcuteCoronary Syndrome. (2014)
  • Author(s): Kawaji T, Shiomi H, Morimoto T, Tamura T, Nishikawa R, Yano M, Tazaki J, Imai M, Saito N, Makiyama T, Shizuta S, Ono K, Kimura T.
  • Journal Title: Circ J. Volume: 78 Pages: 1628-35
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evolution in practice patterns and long-term outcomes of coronary revascularization from bare-metal stent era to drug-eluting stent era in Japan. (2014)
  • Author(s): Shiomi H, Morimoto T, Makiyama T, Ono K, Furukawa Y, Nakagawa Y, Kadota K, Onodera T, Takatsu Y, Mitsudo K, Kita T, Sakata R, Okabayashi H, Hanyu M, Komiya T, Yamazaki F, Nishiwaki N, Kimura T; CREDO-Kyoto Investigators.
  • Journal Title: Am J Cardiol. Volume: 113 Issue: 10 Pages: 1652-9
  • DOI: 10.1016/j.amjcard.2014.02.037
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MicroRNAs and lipoprotein metabolism. (2014)
  • Author(s): 1: Horie-T, Baba-O, Kuwabara-Y, Yokode-M, Kita-T, Kimura-T, Ono-K
  • Journal Title: J of Atherosclerosis and Thrombosis Volume: 21 Pages: 17-22
  • Peer Reviewed
• [Journal Article] Myocardial expression level of neural cell adhesion molecule correlates with reduced left ventricular function in human cardiomyopathy (2014)
  • Author(s): Nagao K, Sowa N, Inoue K, Tokunaga M, Fukuchi K, Uchiyama K, Ito H, Hayashi F, Makita T, Inada T, Tanaka M, Kimura T, and Ono K
  • Journal Title: Circulation; Heart Failur Volume: 7 Issue: 2 Pages: 351-8
  • DOI: 10.1161/circheartfailure.113.000939
  • Peer Reviewed
• [Journal Article] MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice. (2013)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Sowa N, Yahagi N, Shimano H, Matsumura S, Inoue K, Marusawa H, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, Ono K
  • Journal Title: Nat Commun. Volume: 4:2883 Issue: 1 Pages: 2883-2883
  • DOI: 10.1038/ncomms3883
  • NAID: 120005349007
  • Peer Reviewed / Open Access
• [Journal Article] Long-Term Outcomes After Percutaneous Coronary Intervention for Chronic Total Occlusion (from the CREDO-Kyoto Registry Cohort-2) (2013)
  • Author(s): 5: Yamamoto E, Natsuaki M, Morimoto T, Furukawa Y, Nakagawa Y, Ono K, Mitsudo K, Nobuyoshi M, Doi O, Tamura T, Tanaka M, Kimura T; CREDO-Kyoto PCI/CABG Registry Cohort-2 Investigators.
  • Journal Title: Am J Cardiol. Volume: １１２ Issue: 6 Pages: 767-74
  • DOI: 10.1016/j.amjcard.2013.05.004
  • Peer Reviewed
• [Journal Article] Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Plays an Important Role in Vascular Inflammation in Current Smokers. (2013)
  • Author(s): 6: Takanabe-Mori R, Ono K, Wada H, Takaya T, Ura S, Yamakage H, Satoh-Asahara N, Shimatsu A, Takahashi Y, Fujita M, Fujita Y, Sawamura T, Hasegawa K.
  • Journal Title: J Atheroscler Thromb. Volume: 20 Pages: 585-90
  • Peer Reviewed
• [Journal Article] CREDO-Kyoto PCI/CABG registry cohort-2 investigators. Impact of polyvascular disease on clinical outcomes in patients undergoing coronary revascularization : An observation from the CREDO-Kyoto Registry Cohort-2 (2013)
  • Author(s): Morikami Y, Natsuaki M, Morimoto T, Ono K, Nakagawa Y, Furukawa Y, Sakata R, Aota M, Okada Y, Onoe M, Kawasuji M, Koshiji T, Nakajima H, Nishizawa J, Yamanaka K, Yamamoto H, Kimura T
  • Journal Title: Atherosclerosis Volume: 228 Issue: 2 Pages: 426-31
  • DOI: 10.1016/j.atherosclerosis.2013.04.005
  • Peer Reviewed
• [Journal Article] Metastasis-associated protein, S100A4 mediates cardiac fibrosis potentially through the modulation of p53 in cardiac fibroblasts (2013)
  • Author(s): Tamaki Y, Iwanaga Y, Niizuma S, Kawashima T, Kato T, Inuzuka Y, Horie T, Morooka H, Takase T, Akahashi Y, Kobuke K, Ono K, Shioi T, Sheikh SP, Ambartsumian N, Lukanidin E, Koshimizu TA, Miyazaki S, Kimura T
  • Journal Title: J Mol Cell Cardiol Volume: 57 Pages: 72-81
  • DOI: 10.1016/j.yjmcc.2013.01.007
  • Peer Reviewed
• [Journal Article] Influence of initial acute myocardial infarction presentation on the outcome of surgical procedures after coronary stent implantation: a report from the CREDO-Kyoto PCI/CABG Registry Cohort-2. (2013)
  • Author(s): Tokushige A
  • Journal Title: Cardiovasc Interv Ther Volume: 28 Issue: 1 Pages: 45-55
  • DOI: 10.1007/s12928-012-0136-x
  • Peer Reviewed
• [Presentation] シンポジウム2 高血圧における臓器連関 ｢miR-33a/bによる細胞・臓器レベルの脂質代謝制御機構｣ (2015)
  • Author(s): 尾野 亘
  • Organizer: 第44回日本心脈管作動物質学会
  • Place of Presentation: 高松
  • Year and Date: 2015-02-07
  • Invited
• [Presentation] Microrna-33, Located Within Srebf2 Intron, Regulate Fatty Acid Synthesis via Targeting SREBP-1 in vivo (2014)
  • Author(s): 1)Nishino T, Horie T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Ide Y, Nakazeki F, Koyama S, Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: American Heart Association Annual Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] MicroRNA-33b Knock-in Mice for an Intron of Sterol Regulatory Element-Binding Factor 1 (Srebf1) Exhibit Reduced HDL-C in vivo (2014)
  • Author(s): 2)Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Ide Y, Nakazeki F, Koyama S, Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: American Heart Association Annual Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Self-rating Depression Scale Score as an Inverse and Independent Predictor of Successful Smoking Cessation with a Pharmacological Treatment for Nicotine Addiction (2014)
  • Author(s): 3)Wada H, Satoh-Asahara N, Akao M, Abe M, Ono K, Morimoto M, Shimatsu A, Takahashi Y, Hasegawa K.
  • Organizer: American Heart Association Annual Scientific Sessions 2014
  • Place of Presentation: Chicago
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Serum HDL-C decreases in microRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1). (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakano T, Nishiga M, Usami S, Izuhara M, Kimura T, Ono K.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] MicroRNA-33 deficiency reduces atherosclerotic plaque progression in apoE knockout mice. (2014)
  • Author(s): Baba O, Horie T, Ono K, Kuwabara Y, Sowa N, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] MicroRNA-451 is involved in diabetic cardiomyopathy through suppression of the LKB1/AMPK pathway. (2014)
  • Author(s): Kuwabara Y, Horie T, Baba O, Nishiga M, Usami S, Izuhara M, Nakao T, Nishino T, Kita T, Kimura T, Ono K.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] MicroRNA-33, embedded in Srebf2 intron, regulate fatty acid synthesis through targeting SREBP-1 in vivo. (2014)
  • Author(s): Nishino T, Horie T, Baba O, Kuwabara Y, YOkode M, Kita T, Kimura T, Ono K.
  • Organizer: 9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 1)microRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1) exhibit reduced HDL-C in vivo. (2014)
  • Author(s): 尾野 亘、堀江貴裕、西野共達、北 徹、木村 剛
  • Organizer: 第5回 Molecular Cardiovascular Conference Ⅱ
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] 2)飽和脂肪酸により誘導されるmicroRNA-451は、糖尿病性心筋症の増悪因子である。 (2014)
  • Author(s): 桑原康秀、堀江貴裕、馬場 理、出原正康、宇佐美俊輔、中尾哲史、西賀雅隆、西野共達、井手裕也、中関典子、小山智史、木村 剛、尾野 亘
  • Organizer: 第5回 Molecular Cardiovascular Conference Ⅱ
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] MicroRNA-33 deficiency leads to high fat diet- induced obesity and insulin resistance in vivo. (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakano T, Nishiga M, Usami S, Izuhara M, Kimura T, Ono K.
  • Organizer: European Society of Cardiology Congress 2014
  • Place of Presentation: Barcerona
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] MicroRNA-33, embedded in Srebf2 intron, regulate fatty acid synthesis through targeting SREBP-1 in vivo. (2014)
  • Author(s): Nishino T, Horie T, Baba O, Kuwabara Y, YOkode M, Kita T, Kimura T, Ono K.
  • Organizer: European Society of Cardiology Congress 2014
  • Place of Presentation: Barcerona
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] 第9回五島雄一郎賞受賞講演 ｢High impact of microRNA-33 on atherosclerosis and lipid metabolism｣ (2014)
  • Author(s): 尾野 亘
  • Organizer: 第46回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-11
  • Invited
• [Presentation] Featured Session：最先端の病態研究 ｢microRNA-33の動脈硬化・脂質制御における役割｣ (2014)
  • Author(s): 尾野 亘
  • Organizer: 第46回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-10
  • Invited
• [Presentation] MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein-2 (Srebp2) regulates Srebp1 in vivo. (2014)
  • Author(s): Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Sowa N, Yahagi N, Shimano H, Hasegawa K, Kume N Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: The 18th International Vascular Biology Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2014-04-14
• [Presentation] MicroRNA-33 deficiency reduces atherosclerotic plaque progression in apoE knockout mice. (2014)
  • Author(s): Baba O, Horie T, Kuwabara Y, Chujo Y, Watanabe S, Kinoshita M, Horiguchi M, Nakamura T, Chonabayashi K, Hishizawa M, Hasegawa K, Kume N Yokode M, Kita T, Kimura T, Ono K.
  • Organizer: The 18th International Vascular Biology Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2014-04-14
• [Presentation] SREBP-2のイントロンに存在するマイクロRNA-33はSREBP-1の発現を制御する (2014)
  • Author(s): 堀江貴裕
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11
• [Presentation] microRNA-451はLKB1-AMPK経路を抑制することで、ラット心筋細胞において細胞障害を、生体においては高脂肪食誘導性心肥大を誘導する (2014)
  • Author(s): 桑原康秀
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11
• [Presentation] アポE欠損マウスにおいてマイクロRNA-33欠損は動脈硬化進展を抑制する (2014)
  • Author(s): 馬場 理
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11
• [Presentation] 1) MicroRNA-451 Exacerbates Lipotoxicity in Neonatal Rat Cardiomyocytes, and High Fat Diet Induced Cardiac Hypertrophy in Mice through LKB1/AMPK Pathway. (2014)
  • Author(s): Yasuhide Kuwabara,Takahiro Horie, Osamu Baba, Shin Watanabe, Masayasu Izuhara, Tetsushi Nakao, Shunsuke Usami, Tomohiro Nishino, Masataka Nishiga, Toru Kita, Takeshi Kimura, Koh Ono
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: Tokyo
• [Presentation] 2) Shock Reduction Strategies in High Energy Devices. (2014)
  • Author(s): Satoshi Shizuta, Takahiro Doi , Mitsuhiko Yahata , Tetsuma Kawaji , Kentaro Nakai , Hiroaki Sugiyama , Koji Goto , Takeru Makiyama , Koh Ono , Tetsuo Shioi , Takeshi Kimura
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: Tokyo
• [Presentation] 3) MicroRNA-33 Encoded by an Intron of Sterol Regulatory Element-Binding Protein 2 (Srebp2) Regulates Srebp1 in vivo. (2014)
  • Author(s): Takahiro Horie, Tomohiro Nishino, Osamu Baba, Yasuhide Kuwabara, Tetsushi Nakao, Masataka Nishiga, Shunsuke Usami, Masayasu Izuhara, Naoya Sowa, Naoya Yahagi, Hitoshi Shimano, Masayuki Yokode, Toru Kita, Takeshi Kimura, Koh Ono
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: Tokyo
• [Presentation] Use of miRNA for the Detection of Cardiotoxicity. (2013)
  • Author(s): Ono K.
  • Organizer: 4th DIA Cardiac Safety Workshop in Japan
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] Cardiac-specific inhibition of kinase activity in calcium/calmodulin-dependent kinase kinase- leads to accerelated left ventricular remodeling and heart failure after transverse aortic constriction in mice. (2013)
  • Author(s): Ono K.
  • Organizer: Cold spring harbor laboratory meeting. Regulatory and non-coding RNA
  • Place of Presentation: NY, U.S.A.
• [Presentation] MicroRNA encoded by an intron of Srebf2 regulates SREBP-1 in vivo. (2013)
  • Author(s): Koh Ono
  • Organizer: 平成25年度新学術領域「転写代謝システム」第5回総括班会議
  • Place of Presentation: Hyogo
• [Presentation] LncRNAs regulated by pressure-overload induce hypertrophy in neonatal mouse ventricular cardiomyocytes. (2013)
  • Author(s): Kuwabara Y.
  • Organizer: American Heart Association Annual Scientific Sessions 2013
  • Place of Presentation: Dallas, U.S.A.
• [Presentation] マイクロRNA-33欠損はアポE欠損マウスにおいて動脈硬化形成を抑制する (2013)
  • Author(s): 堀江貴裕、馬場 理、木村 剛、尾野 亘
  • Organizer: 第50回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
• [Presentation] HDLコレステロール仮説に関する最近の話題 (2013)
  • Author(s): 尾野 亘
  • Organizer: 第115回日本循環器学会近畿地方会
  • Place of Presentation: 京都
• [Presentation] MicroRNA-33 Deficiency Reduces the Progressio of Atherosclerotic Plaque in ApoE-/- Mice (2013)
  • Author(s): 堀江貴裕、馬場 理、木村 剛、尾野 亘
  • Organizer: 第45回日本動脈硬化学会 イブニングセミナー2
  • Place of Presentation: 東京
• [Presentation] マイクロRNAのバイオマーカーとしての応用 (2013)
  • Author(s): 尾野 亘
  • Organizer: 第45回日本動脈硬化学会 シンポジウム４
  • Place of Presentation: 東京
  • Invited
• [Presentation] miRNAを介した生体における転写環境と代謝の関連の検討 (2013)
  • Author(s): 尾野 亘
  • Organizer: 平成25年度新学術領域「転写代謝システム」第5回総括班会議
  • Place of Presentation: 兵庫
• [Presentation] microRNAと動脈硬化・心不全 (2013)
  • Author(s): 尾野 亘
  • Organizer: 第23回 循環薬理学会
  • Place of Presentation: 福岡
  • Invited
• [Book] Annual Review 循環器2014 (2014)
  • Author(s): 尾野 亘
  • Total Pages: 315
  • Publisher: 中外医学社
• [Remarks] 京都大学循環器内科 心血管疾患分子機構解明グループ
  • URL: http://kyoto-u-cardio.jp/kisokenkyu/metabolic/
• [Remarks] 尾野 亘 講師らの成果が、Nature Communicationsに掲載されました
  • URL: http://tmsystem.tara.tsukuba.ac.jp/?p=2424
• [Remarks] 心血管疾患分子機構解明グループ
  • URL: http://kyoto-u-cardio.jp/kisokenkyu/metabolic/
• [Patent(Industrial Property Rights)] マイクロＲＮＡ溶出型ステント及びそれを用いた管腔治療 (2014)
  • Inventor(s): 尾野 亘、斉藤成達、木村 剛、出原正康
  • Industrial Property Rights Holder: 尾野 亘、斉藤成達、木村 剛、出原正康
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-044463
  • Filing Date: 2014-03-07