Inter-specifc organ formation using blastocyst complementation: creation of rat-pluripotent stem cell-derived heart in mouse
| Project/Area Number |
25670388
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Osaka University |
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Principal Investigator |
LEE JONG-KOOK 大阪大学, 医学(系)研究科(研究院), 寄附講座准教授 (60303608)
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| Research Collaborator |
MIWA Keiko 大阪大学, 大学院医学系研究科・心血管再生医学寄附講座, 特任研究員 (90630476)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 胚盤胞補完 / キメラ動物 / 発生工学 / 臓器創成 / 心臓 / 臓器再生 / 多能性幹細胞 / 心筋転写因子 |
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| Outline of Final Research Achievements |
#1: Nkx2.5 hetero knockout(Nkx2.5+/-) mice were mated and fertilized eggs were harvested at 2.5 dpc. #2: DsRed expressing murine ES cells were prepared. #3: DsRed expressing murine ES cells were injected into the Blastocysts of mated Nkx2.5+/- mice. #4: In vivo and in situ analyses using fluorescent stereoscopic microscope showed that intra-specific chimeric mice were obtained. #5: After the fixation or organs including hearts, in vitro analyses showed that DeRed-positive cells existed relatively confined to the heart in several mice, which suggested the formation of intra-specific chimeric hearts was feasible. However, genotyping of DsRed negative cells (which were originated from fertilized eggs of mated Nkx2.5+/- mice) isolated from embryonic liver showed that Nkx2.5-/- mice was not obtained. #6: Electrophysiological analysis of rat iPS cell-derived cariomyocytes were conducted for the ongoing analysis of the inter-specific chimera between rats and mice.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Excitation propagation in three-dimensional engineered hearts using decellularized extracellular matrix.2014
Author(s)
Yasui H, Lee JK, Yoshida A, Yokoyama T, Nakanishi H, Miwa K, Naito AT, Oka T, Akazawa H, Nakai J, Miyagawa S, Sawa Y, Sakata Y, Komuro I
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Journal Title
Biomaterials
Volume: 35
Pages: 7839-50
Related Report
Peer Reviewed
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[Journal Article] N-glycans: phenotypic homology and structural differences between myocardial cells and induced pluripotent stem cell-derived cardiomyocytes.2014
Author(s)
Kawamura T, Miyagawa S, Fukushima S, Yoshida A, Kashiyama N, Kawamura A, Ito E, Saito A, Maeda A, Eguchi H, Toda K, Lee JK, Miyagawa S, Sawa Y
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Notch activation mediates angiotensin II-induced vascular remodeling by promoting the proliferation and migration of vascular smooth muscle cells2013
Author(s)
Ozasa Y, Akazawa H, Qin Y, Tateno K, Ito K, Kudo-Sakamoto Y, Yano M, Yabumoto C, Naito AT, Oka T, Lee JK, Minamino T, Nagai T, Kobayashi Y, Komuro I
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Journal Title
Hypertens Res.
Volume: 36(10)
Pages: 859-65
DOI
Related Report
Peer Reviewed
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