Three-dimensional reconstruction of bioartificial heart by reprogrammed human cardiomyocytes
| Project/Area Number |
25670389
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Okayama University |
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Principal Investigator |
OH HIDEMASA 岡山大学, 大学病院, 教授 (50372579)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | バイオ人工心臓 / 心臓内幹細胞 / 細胞外マトリックス / 人工心筋細胞 / 血管内皮細胞 / 脱細胞化 / 再播種 |
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| Outline of Final Research Achievements |
The shortage of donors for heart transplantation has become a critical issue to treat the patients with end-stage of cardiac failure. We have decellularized the rat whole heart by using pulsatile perfusion apparatus. Reprogrammed human cardiomyocytes were mixed with endothelial progenitor cells in culture and those were perfused to recellularize the acellular construct. Following 8 to 10 days in continuous perfusion, reseeded cells were engrafted onto the matrix to reconstruct the entire vascular beds and myocardium ex vivo.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Intracoronary Autologous Cardiac Progenitor Cell Transfer in Patients with Hypoplastic Left Heart Syndrome: The TICAP Prospective Phase 1 Controlled Trial.2015
Author(s)
Ishigami S, Ohtsuki S, Tarui S, Ousaka D, Eitoku T, Kondo M, Okuyama M, Kobayashi J, Baba K, Arai S, Kawabata T, Yoshizumi K, Tateishi A, Kuroko Y, Iwasaki T, Sato S, Kasahara S, Sano S, Oh H.
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Journal Title
Circulation Research
Volume: 116
Issue: 4
Pages: 653-664
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by α1-adrenergic receptors.2014
Author(s)
Ogata T, Naito D, Nakanishi N, Hayashi YK, Taniguchi T, Miyagawa K, Hamaoka T, Maruyama N, Matoba S, Ikeda K, Yamada H, Oh H, Ueyama T.
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Journal Title
Proc Natl Acad Sci U S A
Volume: 111
Issue: 10
Pages: 3811-3816
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome.2014
Author(s)
Kobayashi J., Yoshida M., Tarui S., Hirata M., Nagai Y., Kasahara S., Keiji K., Ito H., Sano S., Oh H.
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Journal Title
PLOS One
Volume: 9
Issue: 7
Pages: e102796-e102796
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Intracoronary Cardiac Progenitor Cell Transfer to Treat Univentricular Heart Disease: A Phase 2 Randomized Controlled Clinical Trial (PERSEUS)2014
Author(s)
Suguru Tarui, Shinichi Ohtsuki, Daiki Ousaka, Takahiro Eitoku, Maiko Kondo, Junko Kobayashi, Michihiro Okuyama, Shunta Ishigami, Kenji Baba, Sadahiko Arai, Takuya Kawabata, Ko Yoshizumi, Yosuke Kuroko, Tatsuo Iwasaki, Shuhei Sato, Shingo Kasahara, Shunji Sano, and Hidemasa Oh
Organizer
第78回 日本循環器学会総会学術集会
Place of Presentation
東京
Related Report
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[Presentation] Patient-specific induced pluripotent stem cells recapitulate the models of hypoplastic left heart syndrome.2013
Author(s)
Kobayashi J, Yoshida M, Tarui S, Hirata M, Tateishi A, Arai S, Kasahara S, K Takahashi, K Naruse, Sano S, Oh H.
Organizer
The 21st annual meeting of the Asian society for cardiovascular and thoracic surgery
Place of Presentation
神戸
Related Report
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[Presentation] Lineage induction of patient-specific iPS cells identifies the cardiac transcriptional repression during myocardial growth and patterning in HLHS.2013
Author(s)
Kobayashi J, Yoshida M, Tarui S, Hirata M, Tateishi A, Arai S, Kasahara S, K Takahashi, K Naruse, Sano S, Oh H.
Organizer
TAKAO Symposium
Place of Presentation
東京
Related Report
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[Presentation] Directed differentiation of patient-specific induced pluripotent stem cells identifies the cardiac transcriptional repression during myocardial growth and patterning in hypoplastic left heart syndrome.2013
Author(s)
Kobayashi J, Yoshida M, Tarui S, Hirata M, Tateishi A, Arai S, Kasahara S, K Takahashi, K Naruse, Sano S, Oh H.
Organizer
American Heart Association
Place of Presentation
ダラス
Related Report
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