Efficient and safe reprogramming of fibroblasts into cardiomyocytes with Sendai viral vectors
| Project/Area Number |
25670394
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cardiovascular medicine
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
Ieda Masaki 慶應義塾大学, 医学部, 講師 (70296557)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 心臓 / 再生 / 心筋 / リプログラミング / センダイウイルス |
|---|
| Outline of Final Research Achievements |
Heart disease remains a leading cause of death worldwide, and new therapies are highly demanded. As cardiomyocytes are terminally differentiated cells, regenerative therapy has emerged as an attractive approach for the treatment of heart failure. Direct cardiac reprogramming approach might be a powerful strategy toward this treatment. We reported that a combination of three cardiac-specific transcription factors, Gata4, Mef2c, and Tbx5 (GMT), could directly reprogram fibroblasts into cardiomyocyte-like cells in vitro and in vivo. The aim of this study is to develop new Sendai viral vectors (SeVs) which overexpress reprogramming factors without genomic integration, and to determine the efficiency and safety of cardiac reprograming with SeVs. We generated SeVs vectors containing GMT and found that the vector could efficiently reprogram mouse fibroblasts inot cardiomyocyte-like cells in vitro.
|
Report
(4 results)
Research Products
(47 results)
-
[Journal Article] Effective Cibenzoline Treatment in a Patient With Midventricular Obstruction After Transcatheter Aortic Valve Implantation.2016
Author(s)
Isobe S, Ieda M, Kohno T, Nishiyama T, Maekawa Y, Tsuruta H, Murata M, Yashima F, Yanagisawa R, Tanaka M, Inami S, Nasuno T, Haruyama A, Sano M, Hayashida K, Fukuda K.
-
Journal Title
Circ Heart Fail
Volume: 9
Issue: 1
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Neural crest-derived resident cardiac cells contribute to the restoration of adrenergic function of transplanted heart in rodent.2016
Author(s)
Tamura Y, Sano M, Nakamura H, Ito K, Sato Y, Shinmura K, Ieda M, Fujita J, Kurosawa H, Ogawa S, Nakano S, Matsuzaki M, Fukuda K.
-
Journal Title
Cardiovasc Res.
Volume: 109
Issue: 3
Pages: 350-357
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
[Journal Article] MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures.2014
Author(s)
Mizuha Akiyama, Rie Wada, Kohei Inagawa, Takahiko Nishiyama, Ruri Kaneda, Toru Fukuda, Shu Takeda, Shugo Tohyama, Hisayuki Hashimoto, Yoshifumi Kawamura, Naoki Goshima, Ryo Aeba, Hiroyuki Yamagishi, Keiichi Fukuda, Masaki Ieda
-
Journal Title
EMBO J.
Volume: 33
Issue: 14
Pages: 1565-1581
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Multidisciplinary approach to the treatment of cardiac AA amyloidosis and aortic stenosis due to Castleman's disease: a hybrid therapy with tocilizumab and aortic valve replacement.2014
Author(s)
Shirakawa K, Egashira T, Ieda M, Kawaguchi S, Okamoto K, Kudo M, Yokoyama K, Tsuruta H, Murata M, Mikami S, Anzai A, Hayashida K, Kohno T, Maekawa Y, Sano M, Yozu R, Fukuda K.
-
Journal Title
Int J Cardiol.
Volume: 173
Issue: 2
Pages: e9-e11
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
