Elucidation of malignancy and immaturity in lung small cell carcinoma using a neural stem cell novel factor detected by posttranslational modification proteomics
| Project/Area Number |
25670400
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
Niimori Kanako 熊本大学, 大学院生命科学研究部(医), 助教 (30457600)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 神経幹細胞 / 肺小細胞癌 / リン酸化 / 悪性度 / プロテオミクス |
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| Outline of Final Research Achievements |
This study aims to examine the role of SMF1 in SCLC.First, the histological stain of clinical and pathological human samples revealed that SMF1 was highly expressed in SCLC compared with lung adenocarcinoma and squamous cell carcinoma. Moreover, SMF1 was highly phosphorylated in NSCs, and similarly, it was highly phosphorylated in SCLC. Thus, I created a stable cell line with loss of SMF1 by introducing SMF1-shRNA into a culture of SCLC using the in vitro electroporation method (Fig.3), and examined the nature of cancer in the absence of SMF1 by implanting the cells under the skin of high immunodeficient mice. As a result, in the absence of SMF1, the proliferative ability of the cancer became low, and the size decreased. Additionally, staining of the stable cell line with loss of SMF1 with anti-CD 133 antibody, which is a cancer stem cell marker, and the analysis using flow cytometry revealed that the number of CD 133 positive cells reduced compared with a controll cell line.
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Report
(2 results)
Research Products
(11 results)
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[Journal Article] Insulinoma-Associated protein 1 is a crucial regulator of neuroendocrine differentiation in lung cancer2015
Author(s)
Fujino, Kosuke; Motooka, Yamato; Wael, Hassan; Ali Abdalla, Mohamed; Sato, Yonosuke; Kudoh, Shinji; Hasegawa, Koki; Niimori-Kita, Kanako; Kobayashi, Hironori; Kubota, Ichiro; Wakimoto, Joeji; Suzuki, Makoto; Takaaki, Ito
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Journal Title
The American Journal of Pathology
Volume: 185
Issue: 12
Pages: 3164-3177
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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