| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We generated drug-inducible, proximal tubule-specific megalin knockout mice. Using these mice, we could visualize megalin-independent protein reabsorption in collecting ducts. Reabsorption of retinol-binding protein, a low molecular weight protein, was almost completed at S1-S2 segments of renal cortex. Quantitative contribution of megalin(+) S3 segment (in outer stripe of outer medulla) and megalin(-) collecting ducts for protein reabsorption was relatively very small. Further analysis is required to investigate whether protein reabsorption at collecting ducts elicits inflammatory signals in those cells.
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