Basic research of new epigenetic marker, H4K20ac

• Project/Area Number: 25670410
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Osaka University
• Principal Investigator: Kaimori Jun-Ya 大阪大学, 医学(系)研究科(研究院), 寄附講座准教授 (70527697)
• Co-Investigator(Kenkyū-buntansha): ISAKA Yoshitaka 大阪大学, 大学院医学系研究科, 教授 (00379166) ; 高原 史郎 大阪大学, 医学(系)研究科(研究院), 寄附講座教授 (70179547)
• Co-Investigator(Renkei-kenkyūsha): TAKAHARA Shiro 大阪大学, 大学院医学系研究科, 寄附講座教授 (70179547)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: epigenetics / epigenome / histone modification / gene expression / histone acetylation / epigeneticcs / Chip-seq / 転写促進因子 / 転写抑制因子 / cell hypertrophy

Outline of Final Research Achievements

By suing mass spectmetory analyses, We identified H4K20ac, which was discovered only in plant cells. To understand the function of H4 lysine 20 acetylation (H4K20ac), we have developed a specific monoclonal antibody and performed ChIP-seq analysis using HeLa-S3 cells. H4K20ac was enriched around the transcription start sites (TSSs) of minimally expressed genes and in the gene body of expressed genes, in contrast to most histone acetylation being enriched around the TSSs of expressed genes. The distribution of H4K20ac showed little correlation with known histone modifications, including histone H3 methylations.

Research Products

• [Journal Article] Histone H4 lysine 20 acetylation is associated with gene repression in human cells. (2016)
  • Author(s): Kaimori, J. Y., Maehara, K., Hayashi-Takanaka, Y., Harada, A., Fukuda, M., Yamamoto, S., Ichimaru, N., Umehara, T., Yokoyama, S., Matsuda, R., Ikura, T., Nagao, K., Obuse, C., Nozaki, N., Takahara, S., Takao, T., Ohkawa, Y., Kimura, H., and Isaka, Y.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 24318-24318
  • DOI: 10.1038/srep24318
  • NAID: 120005770760
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 次世代シークエンサーを用いた新規ヒストン修飾の研究 (2013)
  • Author(s): 貝森淳哉
  • Organizer: 日本腎臓学会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 新規エピゲノムマーカーとしてのH4K20アセチル化 (2013)
  • Author(s): 猪阪善隆
  • Organizer: 日本腎臓学会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] New Epigenetic Marker, H4K20ac Related to Transcriptional Suppression (2013)
  • Author(s): 貝森淳哉
  • Organizer: アメリカ腎臓学会
  • Place of Presentation: Atlanta, GA, USA