Identification of antigens in IgG4-related kidney diseases by wheat germ-free protein synthesis system

• Project/Area Number: 25670411
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Ehime University
• Principal Investigator: MASUMOTO Junya 愛媛大学, プロテオサイエンスセンター, 教授 (20334914)
• Co-Investigator(Kenkyū-buntansha): UEHARA Takeshi 信州大学, 医学部, 准教授 (80402121) ; SHIMOJO Hisashi 信州大学, 医学部, 講師 (40324248)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 膜性腎症 / 腎生検 / 病理 / IgG4 / 無細胞蛋白質合成 / H. Pylori / P. Gingibalis / EBウィルス / H.Pylori / P. gingibalis

Outline of Final Research Achievements

Biotinylated protein syntheses from Helicobactor pylori, Porphyromonas gingivalis, and human B-cell lymphoma cell line Raji cells were performed by wheat germ cell-free protein synthesis system. Using these proteins as probes, immunofluorescence staining was performed using biopsy specimens in cases of membranous glomerulonephropathy, interstitial nephritis, and normal control. The fluorescence signal was overlapped with IgG fluorescence signal in some cases. Now the study about that these observations can be seen in other cases is going on.

Research Products

• [Journal Article] Epstein-Barr virus-related MTX-LPD in rheumatoid arthritis patients exhibits a viral pattern of the CD64 and CD35 expression on neutrophils: three case reports. (2015)
  • Author(s): Mokuda S, Miyazaki T, Saeki Y, Masumoto J, Kanno M, Takasugi K.
  • Journal Title: Modern rheumatology Volume: 25 Issue: 1 Pages: 166-168
  • DOI: 10.3109/14397595.2013.875641
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dysregulated mature IL-1β production in familial Mediterranean fever. (2015)
  • Author(s): Migita K, Izumi Y, Fujikawa K, Agematsu K, Masumoto J, Jiuchi Y, Kozuru H, Nonaka F, Shimizu T, Nakamura T, Iwanaga N, Furukawa H, Yasunami M, Kawakami A, Eguchi K.
  • Journal Title: Rheumatology (Oxford) Volume: 54 Issue: 4 Pages: 660-665
  • DOI: 10.1093/rheumatology/keu359
  • Peer Reviewed
• [Journal Article] Development of tumor-specific caffeine-potentiated chemotherapy using a novel drug delivery system with Span 80 nano-vesicles. (2015)
  • Author(s): Nakata H, Miyazaki T, Iwasaki T, Nakamura A, Kidani T, Sakayama K, Masumoto J, Miura H
  • Journal Title: Oncology reports. Volume: 33 Issue: 4 Pages: 1593-1598
  • DOI: 10.3892/or.2015.3761
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IL-18 serum concentration is markedly elevated in typical familial Mediterranean fever with M694I mutation and can distinguish it from atypical type (2015)
  • Author(s): Yamazaki T, Shigemura T, Kobayashi N, Honda K, Yazaki M, Masumoto J, Migita K, Agematsu K
  • Journal Title: Mod Rheumatol Volume: 印刷中
  • Peer Reviewed
• [Journal Article] IL-1 as a target in inflammation (2015)
  • Author(s): Ito Y, Kaneko N, Iwasaki T, Morikawa S, Kaneko K, Masumoto J.
  • Journal Title: Endocr Metab Immune Disord Drug Targets Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Corresponding Author of manuscript "The proto-oncogene survivin splice variant 2B is induced by PDGF and leads to cell proliferation in rheumatoid arthritis fibroblast-like synoviocyte (2015)
  • Author(s): Mokuda S, Miyazaki T, Ito Y, Yamasaki S, Inoue H, Guo Y, Kong W, Kanno M, Takasugi K, Sugiyama E, Masumoto J
  • Journal Title: Scientific Reports Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Defect of suppression of inflammasome-independent interleukin-8 secretion from SW982 synovial sarcoma cells by familial Mediterranean fever-derived pyrin mutations. (2014)
  • Author(s): Sugiyama R, Agematsu K, Migita K, Nakayama J, Mokuda S, Ogura F, Haraikawa K, Okumura C, Suehiro S, Morikawa S, Ito Y, Masumoto J
  • Journal Title: Mol Biol Rep Volume: 41 Issue: 1 Pages: 545-553
  • DOI: 10.1007/s11033-013-2890-y
  • Peer Reviewed
• [Journal Article] Tocilizumab improved clinical symptoms of a patient with systemic tophaceous gout who had symmetric polyarthritis and fever: An alternative treatment by blockade of interleukin-6 signaling (2014)
  • Author(s): Mokuda S, Kanno M, Takasugi K, Okumura C, Ito Y, Masumoto J
  • Journal Title: SAGE Open Medical Case Reports Volume: 2 Pages: 13519774-13519774
  • DOI: 10.1177/2050313x13519774
  • Peer Reviewed
• [Journal Article] Familial Mediterranean fever: genotype-phenotype correlations in Japanese patients. (2014)
  • Author(s): Migita K, Agematsu K, Yazaki M, Nonaka F, Nakamura A, Toma T, Kishida D, Uehara R, Nakamura Y, Jiuchi Y, Masumoto J, Furukawa H, Ida H, Terai C, Nakashima Y, Kawakami A, Nakamura T, Eguchi K, Yasunami M, Yachie A.
  • Journal Title: Medicine (Baltimore) Volume: 93 Issue: 3 Pages: 158-164
  • DOI: 10.1097/md.0000000000000029
  • Peer Reviewed
• [Journal Article] Serum amyloid A induces NLRP-3-mediated IL-1β secretion in neutrophils. (2014)
  • Author(s): Migita K, Izumi Y, Jiuchi Y, Kozuru H, Kawahara C, Nakamura M, Nakamura T, Agematsu K, Masumoto J, Yasunami M, Kawakami A, Eguchi K.
  • Journal Title: PLoS One Volume: 9 Issue: 5 Pages: e96703-e96703
  • DOI: 10.1371/journal.pone.0096703
  • Peer Reviewed / Open Access
• [Journal Article] Microvasculature of carotid atheromatous plaques: hemorrhagic plaques have dense microvessels with fenestrations to the arterial lumen (2014)
  • Author(s): Mie Kurata
  • Journal Title: J Stroke Cerebrovasc Dis Volume: 13 Issue: 6 Pages: 1-7
  • DOI: 10.1016/j.jstrokecerebrovasdis.2013.12.003
  • Peer Reviewed
• [Journal Article] Microvasculature of Carotid Atheromatous Plaques: Hemorrhagic Plaques Have Dense Microvessels with Fenestrations to the Arterial Lumen. (2014)
  • Author(s): Kurata M, Nose M, Shimazu Y, Aoba T, Kohada Y, Yorioka S, Suehiro S, Fukuoka E, Matsumoto S, Watanabe H, Kumon Y, Okura T, Higaki J, Masumoto J.
  • Journal Title: J Stroke Cerebrovasc Dis Volume: S1052-3057 Pages: 507-507
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus-related MTX-LPD in rheumatoid arthritis patients exhibits a viral pattern of the CD64 and CD35 expression on neutrophils: Three case reports. (2014)
  • Author(s): Mokuda S, Miyazaki T, Saeki Y, Masumoto J, Kanno M, Takasugi K.
  • Journal Title: Mod Rheumatol Volume: in press
  • Peer Reviewed
• [Journal Article] The contribution of SAA1 polymorphisms to Familial Mediterranean fever susceptibility in the Japanese population. (2013)
  • Author(s): Migita K, Agematsu K, Masumoto J, Ida H, Honda S, Jiuchi Y, Izumi Y, Maeda Y, Uehara R, Nakamura Y, Koga T, Kawakami A, Nakashima M, Fujieda Y, Nonaka F, Eguchi K, Furukawa H, Nakamura T, Nakamura M, Yasunami M.
  • Journal Title: PLoS One Volume: 8 Issue: 2 Pages: e55227-e55227
  • DOI: 10.1371/journal.pone.0055227
  • Peer Reviewed
• [Journal Article] Vibrio parahaemolyticus effector proteins suppress inflammasome activation by interfering with host autophagy signaling (2013)
  • Author(s): Naomi Higa, Claudia Toma, Yukiko Koizumi, Noboru Nakasone, Toshitsugu Nohara and Toshihiko Suzuki
  • Journal Title: PloS Pathogen Volume: 9(1) Issue: 1 Pages: e1003142-e1003142
  • DOI: 10.1371/journal.ppat.1003142
  • Peer Reviewed
• [Journal Article] IgG4-related disease-like fibrosis as an indicator of IgG4-related lymphadenopathy. (2013)
  • Author(s): Uehara T, Masumoto J, Yoshizawa A, Kobayashi Y, Hamano H, Kawa S, Oki K, Oikawa N, Honda T, Ota H.
  • Journal Title: Ann Diagn Pathol Volume: 17 Issue: 5 Pages: 416-20
  • DOI: 10.1016/j.anndiagpath.2013.04.010
  • Peer Reviewed
• [Journal Article] The inflammasome. (2013)
  • Author(s): Okumura C, Haraikawa K, Suehiro S, Ito Y, Masumoto J.
  • Journal Title: Nihon Rinsho Volume: 71 Pages: 1497-1504
  • NAID: 10029897864
  • Peer Reviewed
• [Presentation] Defect of suppression of inflammasome-independent interleukin-8 secretion from sw982 synovial sarcoma cells by familial mediterranean fever-derived pyrin mutations (2014)
  • Author(s): Masumoto J
  • Organizer: 16th Biennial Meeting of the European Society for Immunodeficiencies
  • Place of Presentation: Plagh, Czech Republic.
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] 自己炎症疾患とインフラマソーム (2013)
  • Author(s): 増本純也
  • Organizer: 第５７回日本リウマチ学会総会・学術集会/第２２回国際リウマチシンポジウム
  • Place of Presentation: 国立京都国際会館, 京都, 日本
  • Invited
• [Remarks] 愛媛大学 プロテオサイエンスセンター 病理学部門
  • URL: http://www.pros.ehime-u.ac.jp/section/09.html