| Project/Area Number |
25670445
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KUROKAWA Mineo 東京大学, 医学部附属病院, 教授 (80312320)
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| Co-Investigator(Renkei-kenkyūsha) |
KATAOKA Keisuke 東京大学, 医学部附属病院, 特任助教 (90631383)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 骨髄異形成症候群 / 治療抵抗性 / RNAiスクリーニング / 網羅的ゲノム解析 |
|---|
| Research Abstract |
The aim of this study is to elucidate the mechanism of resistance to treatment in myelodysplastic syndrome (MDS) patients by using RNA-interference technologies. We employed the small-hairpin RNA plasmid library from the Decipher (Cellcta, Mountain View, CA, USA) and transduced into MDS-derived human cell lines. Transduced cell lines were treated with azacitidine, a first-line drug for MDS patients, for 30 days. Then, we isolated genomic DNA and detected specific barcode sequences for each shRNA plasmid with next-generation sequencing. By comparing the clonal composition with or without azacitidine treatment, we identified 158 candidate genes that may induce the azacitidine resistance in MDS.
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