Evaluation of aging by using cell-free nucleic acid
Project/Area Number |
25670451
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Osaka University |
Principal Investigator |
KANAKURA YUZURU 大阪大学, 医学(系)研究科(研究院), 教授 (20177489)
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Co-Investigator(Kenkyū-buntansha) |
ORITANI Kenji 大阪大学, 大学院医学系研究科, 准教授 (70324762)
ICHII Michiko 大阪大学, 大学院医学系研究科, 寄附講座助教 (30633010)
SAITOH Norimitsu 大阪大学, 大学院医学系研究科, 特任助教(常勤) (30597399)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 老化 / 造血幹細胞 / 分化 / Wnt / クロマチン / リンパ球 / SATB1 / sFRP5 / 内科 |
Outline of Final Research Achievements |
We established transgenic chimera mice to produce soluble Frizzled-related protein (sFRP)-5 under the immunoglobulin-kappa promoter. Overexpression of sFRP-5 inhibited B lymphocyte differentiation after the level of common lymphoid progenitors, leading to massive decrease of B lymphocytes in spleen. On the other hand, sFRP-5 deficient mice showed significant decrease of hematopoietic stem cells (HSC) when the mice were stimulated by estrogen. We established reporter mice, which monitor Special AT-rich sequence binding protein 1 (SATB1) gene expression. HSC contained two populations divided by SATB1-positivity, and the SATB1-positive cells had less HSC characters and more lymphocyte production than SATB1-negative cells. Therefore, we successfully clarified in vivo roles of sFRP-5 and SATB1 in the lymphocyte production. Our results provide useful information to understand HSC aging.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Estrogen-inducible sFRP5 inhibits early B-lymphopoiesis in vivo, but not during pregnancy.2015
Author(s)
Yokota T, Oritani K, Sudo T, Ishibashi T, Doi Y, Habuchi Y, Ichii M, Fukushima K, Okuzaki D, Tomizuka K, Yamawaki K, Kakitani M, Shimono A, Morii E, Kincade PW, Kanakura Y
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Journal Title
Eur J Immunol
Volume: 印刷中
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The Satb1 protein directs hematopoietic stem cell differentiation toward lymphoid lineages.2013
Author(s)
Satoh Y, Yokota T, Sudo T, Kondo M, Lai A, Kincade PW, Kouro T, Iida R, Kokame K, Miyata T, Habuchi Y, Matsui K, Tanaka H, Matsumura I, Oritani K, Kohwi-Shigematsu T, Kanakura Y.
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Journal Title
Immunity
Volume: 38
Issue: 6
Pages: 1105-1115
DOI
Related Report
Peer Reviewed
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[Presentation] SFRP5 inhibits early stages of B-cell differentiation and modulates estrogen-related changes in the immune system
Author(s)
Yokota T, Oritani K, Sudo T, Ishibashi T, Doi Y, Habuchi Y, Ichii M, Okuzaki D, Tomizuka K, Yamawaki K, Kakitani M, Shimono A, Morii E, Kincade PW, Kanakura Y
Organizer
The American Society of Hematology 55th Annual Meeting
Place of Presentation
New Orleans, LA, USA
Related Report
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