| Project/Area Number |
25670454
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MIYATA TOSHIYUKI 独立行政法人国立循環器病研究センター, 研究所, 部長 (90183970)
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| Co-Investigator(Kenkyū-buntansha) |
BANNO Fumiaki 独立行政法人国立循環器病研究センター, 研究所, 研究員 (00373514)
TASHIMA Yuko 大阪大学, 微生物病研究所, 助教 (10423104)
MARUYAMA Keiko 独立行政法人国立循環器病研究センター, 研究所, 流動研究員 (30712624)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 血管内皮細胞 / 遺伝子発現 / トロンボモジュリン / プロテインC受容体 / マウス / トランスクリプトーム / プロテイン C 受容体 / 血管内皮細胞プロテインC受容体 / トランスジェニックマウス / トランスクリプトーム解析 / Bac-TRAP / ライブイメージング |
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| Outline of Final Research Achievements |
Endothelia in the arterial and venous circulation are heterogeneous in nature. In order to understand their heterogeneity, we developed novel BAC transgenic mice with cDNA encoding fusion proteins of a fluorescent protein, EGFP or mCherry, and a ribosomal protein L10a under control of the endothelial cell-specific thrombomodulin (TM) or endothelial cell protein C receptor (EPCR) promoter. We compared the expression intensities of transcribing endothelial-specific markers before and after immunoprecipitation with anti-EGFP antibody or anti-mCherry antibody and found that those markers are increased merely 2-3 fold after immunoprecipitation. We further examined the immunofluorescence staining of the heart and lung of the transgenic mice using anti-EGFP antibody or anti-mCherry antibody but failed to detect the endothelia positive for the antibody staining. We concluded that novel BAC transgenic mice we established showed low expression of EGFP-L10a or mCherry-L10a in endothelia.
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