A humoral lipid preventing from mouse anaphylactic death

Research Project

Project/Area Number	25670456
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Collagenous pathology/Allergology
Research Institution	Akita University
Principal Investigator	Masuda Yutaka 秋田大学, 医学部, 講師 (20199706)
Co-Investigator(Renkei-kenkyūsha)	KOYOTA SOUICHI 秋田大学, バイオサイエンス教育・研究センター分子医学部門, 准教授 (80400480)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	アナフィラキシー / IgG / 糖鎖
Outline of Final Research Achievements	Anaphylaxis gives mice severe stress killing them. They could produce adaptogenic substance for coping the stress. In the present study, the adaptogenic substance preventing from mouse anaphylactic death was examined. Six-week-old male ddY mice were initially immunized with 1mg/kg of ovalbumin and second immunized with the same dosage 10 days after the initial immunization. The mice further immunized with the same dosage at 5 days or 15 days after the second immunization. All of the former were killed by the anaphylactic response, but none of the latter were. Although reactivity of fucosylated antibodies inducing anaphylactic response was not different in the former sera and the latter sera, a lipid preventing from anaphylactic death was found in fraction eluted with 300mM NaCl of the latter sera. The present findings suggest that the lipid had an adaptogenic activity against severe stress induced by anaphylactic response, and was produced in late phase of the second immune response.