Challenge the infectious diseases and cancers using innovative array technology: Development of hTEC10

• Project/Area Number: 25670463
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: University of Toyama
• Principal Investigator: MURAGUCHI Atsushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20174287)
• Co-Investigator(Renkei-kenkyūsha): KISHI Hiroyuki 富山大学, 大学院医学薬学研究部(医学), 准教授 (60186210) ; OZAWA Tatsuhiko 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105) ; KOBAYASHI Eiji 富山大学, 大学院医学薬学研究部(医学), 助教 (70459733)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 感染症 / 癌治療 / リンパ球チップ / 抗体 / T cell receptor / T Cell Receptor

Outline of Final Research Achievements

We developed a rapid and efficient antibody gene cloning system from a human single B lymphocyte. In this study, by applying the system to T cell receptor, we have developed a rapid and efficient T cell receptor cloning system (hTEC10: human T cell receptor efficient cloning within 10 days),in which we are able to detect an antigen-specific T cells, to clone efficiently TCR-alpha and TCR-beta chain from a single T cell, and finally evaluate the function of T cell receptor by examining reactivity of specialized T cell line (TCR negative cell line). All of the process can be done within 10 days. Using this hTEC10 system, we have been successful in cloning the functional TCRs with high killer activity from peripheral blood lymphocytes of patients with hepatocyte cell carcinoma. This system may contribute TCR gene therapy of infectious diseases as well as various cancers.

Research Products

• [Journal Article] Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4 + T cells (2015)
  • Author(s): Z. Mou, J. Li, T. Boussoffara, H. Kishi, H. Hamana, P. Ezzati, C. Hu, W. Yi, D. Liu, F. Khadem, I. Okwor, P. Jia, K. Shitaoka, S. Wang, M. Ndao, C. Petersen, J. Chen, S. Rafati, H. Louzir, A. Muraguchi, J. a Wilkins, and J. E. Uzonna,
  • Journal Title: Science Translational Medicine Volume: 7 Issue: 310 Pages: 1-13
  • DOI: 10.1126/scitranslmed.aac5477
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Immunological features of T cells induced by human telomerase reverse transcriptase-derived peptides in patients with hepatocellular carcinoma. (2015)
  • Author(s): E Mizukoshi, H Nakagawa, M Kitahara, T Yamashita, K Arai, H Sunagozaka, K Fushimi, E Kobayashi, H Kishi, A Muraguchi, S Kaneko.
  • Journal Title: Cancer Lett Volume: 364 Issue: 2 Pages: 98-105
  • DOI: 10.1016/j.canlet.2015.04.031
  • Peer Reviewed / Open Access
• [Journal Article] Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay (2015)
  • Author(s): Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 11333-11333
  • DOI: 10.1038/srep11333
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated Natural Killer (NK) Cells (2015)
  • Author(s): Venkateswara R. Simhadri, Milena Dimitrova, John L. Mariano, Olatz Zenarruzabeitia, Weimin Zhong, Tatsuhiko Ozawa, Atsushi Muraguchi, Hiroyuki Kishi, Maryna C. Eichelberger, Francisco Borrego
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0124677-e0124677
  • DOI: 10.1371/journal.pone.0124677
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monoclonal Antibody Against Citrullinated Peptides Obtained From Rheumatoid Arthritis Patients Reacts With Numerous Citrullinated Microbial and Food Proteins (2015)
  • Author(s): Reina Tsuda, Tatsuhiko Ozawa, Eiji Kobayashi, Hiroshi Hamana, Hirofumi Taki, Kazuyuki Tobe, Eiji Sugiyama, Masahiro Iwamoto, Johji Imura, Hiroyuki Kishi, Atsushi Muraguchi
  • Journal Title: Arthritis & Rheumatology Volume: 67 Issue: 8 Pages: 2020-2031
  • DOI: 10.1002/art.39161
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A chimeric antigen receptor for TRAIL-receptor 1 induces apoptosis in various types of tumor cells. (2014)
  • Author(s): Kobayashi E, Kishi H, Ozawa T, Hamana H, Nakagawa H, Jin A, Lin Z, Muraguchi A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 453 Issue: 4 Pages: 798-803
  • DOI: 10.1016/j.bbrc.2014.10.024
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Retroviral vectors for homologous recombination provide efficient cloning and expression in mammalian cells (2014)
  • Author(s): Kobayashi E, Kishi H, Ozawa T, Horii M, Hamana H, Nagai T, Muraguchi A
  • Journal Title: Biochem Biophys Res Commun Volume: 444 Issue: 3 Pages: 319-324
  • DOI: 10.1016/j.bbrc.2014.01.049
  • Peer Reviewed
• [Journal Article] A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy. (2014)
  • Author(s): Kobayashi E, Kishi H, Muraguchi A.
  • Journal Title: Oncoimmunology Volume: 3
  • Peer Reviewed
• [Journal Article] A new cloning and expression system yields and validates TCRs from blood lymphocytes of patients with cancer within 10 days. (2013)
  • Author(s): Kobayashi E., et al
  • Journal Title: Nature Medicne Volume: 19 Issue: 11 Pages: 1542-1546
  • DOI: 10.1038/nm.3358
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): 大永崇、嶋田裕、森山亮仁、岸裕幸、小幡勤、高田耕児、奥村知之、長田拓哉、村口篤、塚田一博
  • Journal Title: Biomedical Microdevices Volume: 15 Issue: 4 Pages: 611-616
  • DOI: 10.1007/s10544-013-9775-7
  • Peer Reviewed
• [Presentation] Comprehensive T-Cell Receptor Repertoire Analysis Using Deep Sequencing and Single Cell Cloning Reveals Extreme Oligoclonality of Ex Vivo Expanded Cytomegalovirus-Reactive Cytotoxic T-Cells. (2015)
  • Author(s): Miyama T, Kawase T, Kitaura K, Chishaki R, Shibata M, Oshima K, Hamana H, Kishi H, Kuzushima K, Saji H, Suzuki R, Ichinohe T.
  • Organizer: The 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orlando（米国）
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] ISAAC法を用いた関節リウマチ患者由来自己抗体の単離とその自己抗体が認識する新しい自己抗原の同定． (2015)
  • Author(s): 小澤龍彦，津田玲奈，浜名 洋，多喜博文，戸邉一之，杉山英二，岩本雅弘，井村穣二，岸 裕幸，村口 篤．
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] コムギ無細胞系を用いた膜タンパク質間トランス相互作用解析技術． (2015)
  • Author(s): 栄谷紘一，竹田浩之，小澤龍彦，村口 篤，澤崎達也．
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 抗膜タンパク質ウサギ高親和性モノクローナル抗体のエピトープ配列を由来とする新規アフィニティタグの開発． (2015)
  • Author(s): 矢野智也，竹田浩之，小澤龍彦，村口 篤，澤崎達也．
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] ISAAC法による日本脳炎ワクチン被接種者末梢血単核球からのヒト抗ウエストナイルウイルス中和モノクローナル抗体の樹立． (2015)
  • Author(s): 正木秀幸，小澤龍彦，高崎智彦，青山幾子，弓指孝博，小西英二，岸 裕幸，村口 篤．
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Assessment of T cell receptor function. (2015)
  • Author(s): Kishi H, Hamana H, Shitaoka K, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Development of an NFAT-luciferase reporter system for screening of antigen-specific TCRs using non-T cells. (2015)
  • Author(s): Hamana H, Kishi H, Shitaoka K, Piao X, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Identification of tumor-specific T cell receptors from primary tumor-infiltrating lymphocytes (TILs) obtained from B16F10 melanoma-bearing mice. (2015)
  • Author(s): Shitaoka K, Kishi H, Hamana H, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Identification of T-cell receptors of the CD8+ T cells in response to HLA-A24 restricted epitopes from hepatitis B virus. (2015)
  • Author(s): Tamai T, Kishi H, Shitaoka K, Hamana H, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Reactivity of a monoclonal ACPA obtained from RA patient with numerous citrullinated antigens. (2015)
  • Author(s): Ozawa T, Tsuda R, Hamana H, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] TRAIL receptor-1 IgM antibodies strongly induced apoptosis in cancer cells. (2015)
  • Author(s): Piao X, Ozawa T, Kishi H, Shitaoka K, Hamana H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Isolation of monoclonal anti-TRIM21/Ro52 autoantibodies from collagen disease-patients with interstitial lung disease and their reactivity to the peptide library. (2015)
  • Author(s): Obi M, Ozawa T, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology. (2015)
  • Author(s): Masaki H, Ozawa T, Takasaki T, Kishi H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Single Cell Analysis of Antigen Receptors of Lymphocytes for Tumor Immunology and Immunotherapy. (2015)
  • Author(s): Kishi H.
  • Organizer: The Korean Association of Immunologists 2015 Fall Conference
  • Place of Presentation: Seoul（韓国）
  • Year and Date: 2015-11-12
  • Int'l Joint Research / Invited
• [Presentation] Comprehensive analysis of CMV-specific T-cell repertoire by deep sequencing and single cell cloning． (2015)
  • Author(s): 美山貴彦，川瀬孝和，北浦一孝，樗木 錬，柴田真志，大島久美，浜名 洋，岸 裕幸，葛島清隆，佐治博夫，鈴木隆二，一戸辰夫．
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2015-10-16
• [Presentation] プライマリー腫瘍浸潤リンパ球を用いた腫瘍特異的T細胞受容体同定の試み． (2015)
  • Author(s): 岸 裕幸，村口 篤．
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 悪性リンパ腫における腫瘍浸潤CD8+Tリンパ球の解析． (2015)
  • Author(s): 村橋睦了，岸 裕幸，松本太一，原 周司，村口 篤，田村和夫，谷憲三朗．
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 悪性リンパ腫における腫瘍浸潤CD8+Tリンパ球の解析． (2015)
  • Author(s): 岸 裕幸，村橋睦了，浜名 洋，松本太一，原 周司，猪狩洋介，石塚賢治，高松 泰，田村和夫，谷憲三朗，村口 篤．
  • Organizer: 第7回血液疾患免疫療法研究会学術集会
  • Place of Presentation: 東京大学伊藤謝恩ホール（東京都文京区）
  • Year and Date: 2015-09-26
• [Presentation] 悪性リンパ腫における腫瘍浸潤CD8+Tリンパ球の解析． (2015)
  • Author(s): 村橋睦了，松本太一，浜名 洋，勝屋弘雄，猪狩洋介，石塚賢治，原 周司，岸 裕幸，高松 泰，田村和夫．
  • Organizer: 第13回日本臨床腫瘍学会学術集会
  • Place of Presentation: ロイトン札幌（北海道札幌市）
  • Year and Date: 2015-07-16
• [Presentation] Distinct pattern of the binding of human monoclonal anti-cyclic citrullinated peptide antibody to joint synovium in between osteoarthritis and rheumatoid arthritis. (2015)
  • Author(s): Iwamoto M, Honne K, Hanai S, Sekiya H, Ozawa T, Kishi H, Muraguchi A, Minota S.
  • Organizer: 2015 Henry Kunkel Meeting
  • Place of Presentation: Annecy（フランス）
  • Year and Date: 2015-04-14
  • Int'l Joint Research
• [Presentation] Cis-interaction of TCR and antigen/MHC class I complexes on a CD8+ T cell. (2014)
  • Author(s): Kishi H, Hamana H, Tajiri K, Ozawa T, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Rapid generation of rabbit monoclonal antibodies against G-protein coupled receptor and application of these antibodies. (2014)
  • Author(s): Ozawa T, Piao X, Kishi H, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Development of simple and efficient method for amplification of TCR αβ cDNA from single human T cells. (2014)
  • Author(s): Hamana H, Kishi H, Ozawa T, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Identification of T cell antigen by an artificial reporter-cell system. (2014)
  • Author(s): Piao X, Kobayashi E, Kishi H, Hamana H, Ozawa T, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology. (2014)
  • Author(s): Masaki H, Ozawa T, Takasaki T, Kishi H, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] GPCR特異的ウサギモノクローナル抗体の迅速作製法の開発． (2014)
  • Author(s): 小澤龍彦，朴 秀虹，小林栄治，竹田浩之，澤崎達也，岸 裕幸，村口 篤．
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Functional features of antigen-specific T-cell receptors reflected clinical responses of α-fetoprotein-derived peptides vaccine treatment for advanced hepatocellular carcinoma. (2014)
  • Author(s): Nakagawa H, Mizukoshi E, Kobayashi E, Terashima T, Kitahara M, Iida N, Kishi H, Muraguchi A, Kaneko S.
  • Organizer: The 65th Annual Meeting of the American Association for the Study of Liver Diseases
  • Place of Presentation: Boston
  • Year and Date: 2014-11-07 – 2014-11-11
• [Presentation] 単一B細胞からの抗体の作製と評価． (2014)
  • Author(s): 村口 篤．
  • Organizer: 第87回日本生化学会大会フォーラム
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] メラノーマ患者および健常人PBMC中のCD4+ T細胞の単一細胞レベルでのレパートリー解析． (2014)
  • Author(s): 岸 裕幸，小林栄治，杉山大介，西川博嘉，坂口志文，村口 篤．
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] メラノーマ患者および健常人の末梢血CD4+ T細胞の単一細胞レベルでのレパートリー解析． (2014)
  • Author(s): 岸 裕幸，浜名 洋，小林栄治，杉山大介，西川博嘉，鬼塚志乃，坂口志文，村口 篤．
  • Organizer: 第6回血液疾患免疫療法研究会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-09-06
• [Presentation] がんペプチドワクチン療法におけるTCRレパートリーの経時的変化の検討． (2014)
  • Author(s): 中河秀俊，水腰英四郎，清家拓哉，山宮大典，米島 淳，稲田悠記，木田明彦，梶喜一郎，玉井利克，熊谷将史，寺島健志，北原征明，飯田宗穂，小林栄治，岸 裕幸，村口 篤，金子周一．
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: 松山
  • Year and Date: 2014-07-30 – 2014-08-01
• [Presentation] Rapid generation of human monoclonal Abs and TCRs from single human lymphocyte: application for gene therapy of infectious diseases and cancer. (2014)
  • Author(s): Muraguchi A.
  • Organizer: The 6th China-Russia International Conference on Medicine
  • Place of Presentation: Harbin
  • Year and Date: 2014-07-01 – 2014-07-03
  • Invited
• [Presentation] Detection of antigen-stimulated cytokine-secretion in human T-cells at single cell levels on a live cell chip. (2013)
  • Author(s): Kishi H, Jin A, Hamana H, Tajiri K, Hatakeyama S, Kobayashi E, Ozawa T, Nagai T, Muraguchi A.
  • Organizer: Measuring Antigen-Specific Immune Responses (MASIR) 2013
  • Place of Presentation: Dubrovnik
• [Presentation] Cloning of human antigen-specific TCRs can confer the candidates for cancer gene therapy. (2013)
  • Author(s): Kobayashi E, Mizukoshi E, Kishi H, Hamana H, Nagai T, Ozawa T, Nakagawa H, Jin A, Kaneko S, Muraguchi A.
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: Milan
• [Presentation] A monoclonal CCP-antibody derived from RA patients binds to several citrullinated antigens in the human cellular components. (2013)
  • Author(s): Ozawa T, Tuda R, Kobayashi E, Hounoki H, Shinoda K, Taki H, Tobe K, Kishi H, Muraguchi A.
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: Milan
• [Presentation] 末梢血リンパ球中の抗原特異的CD8+T細胞およびT細胞受容体の網羅的・迅速解析． (2013)
  • Author(s): 岸 裕幸，小林栄治，水腰英四郎，小澤龍彦，浜名 洋，長井輝美，中河秀俊，金 艾順，金子周一，村口 篤．
  • Organizer: 第5回造血器腫瘍免疫療法研究会学術集会
  • Place of Presentation: 名古屋
• [Presentation] リンパ球チップを用いたヒトモノクローナル抗体の迅速作製． (2013)
  • Author(s): 村口 篤，小澤龍彦，岸 裕幸．
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] がん抗原特異的TCR遺伝子の迅速クローニング法． (2013)
  • Author(s): 小林栄治，水腰英四郎，岸 裕幸，金子周一，村口 篤．
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] リン酸化ペプチド及びGPCR特異的ウサギモノクローナル抗体の迅速作製法の開発． (2013)
  • Author(s): 13) 小澤龍彦，朴 秀虹，小林栄治，竹田浩之，澤崎達也，周 越，櫻井宏明，安東嗣修，金 艾順，岸 裕幸，村口 篤．
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Cis-interaction of TCR and antigenic peptides with MHC class I molecules on a CD8+ T-cell. (2013)
  • Author(s): Kishi H, Jin A, Hamana H, Tajiri K, Kobayashi E, Ozawa T, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] Rapid generation of rabbit monoclonal antibodies against phosphorylated peptides and G-protein coupled receptor. (2013)
  • Author(s): Ozawa T, Piao X, Kobayashi E, Jin A, Kishi H, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] Cloning and functional analysis of human telomerase reverse transcriptase (hTERT)-specific TCRs from peptide-vaccinated patients. (2013)
  • Author(s): Kobayashi E, Nakagawa H, Hamana H, Kishi H, Ozawa T, Jin A, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] hTEC4 system that enable us to clone TCR cDNA from antigen specific single T cells with in 4 days. (2013)
  • Author(s): Hamana H, Kobayashi E, Kishi H, Ozawa T, Nakagawa H, Jin A, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] Construction of T cell antigen-exploring system. (2013)
  • Author(s): Piao X, Hamana H, Kishi H, Kobayashi E, Ozawa T, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] Functional analysis of anti-cyclic citrullinated protein antibodies derived from rheumatoid arthritis. (2013)
  • Author(s): Tsuda R, Ozawa T, Kobayashi E, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] Comparison of α-fetoprotein-specific TCR repertoires from hepatocellular carcinoma patients after peptide vaccine treatment and healthy donors. (2013)
  • Author(s): Nakagawa H, Kobayashi E, Hamana H, Ozawa T, Kishi H, Muraguchi A.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉