Novel diagnostic method for infectious disease using next generation sequencing
| Project/Area Number |
25670468
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HAYASHI Tetsuya 九州大学, 大学院医学研究院, 教授 (10173014)
OKAYAMA Akihiko 宮崎大学, 医学部, 教授 (70204047)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | メタゲノム / 感染症 / 未培養 / 病原微生物 / 感染症診断 / 次世代シーケンサ / 細菌 |
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| Outline of Final Research Achievements |
In the diagnosis and treatment of infectious diseases, the time that required for identification of the pathogen from patients, directly faces to the problem of the starting point for appropriate treatment and affects on patient prognosis. The exhaustive detection method for pathogens using the next generation sequencers without cultivation for isolation was developed. In the case of analyses such as blood, the current DNA purification method contaminates the large amount of host-derived DNA. It interferes to get enough of the sequence information of the pathogen of interest. Therefore, we developed a DNA purification method and identification method for efficiently acquiring information of the pathogen.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Genetic relatedness and virulence properties of enteropathogenic Escherichia coli strains of serotype O119:H6 expressing localized adherence or localized and aggregative adherence-like patterns on HeLa cells.2016
Author(s)
Garcia BG, Ooka T, Gotoh Y, Vieira MA, Yamamoto D, Ogura Y, Girão DM, Sampaio SC, Melo AB, Irino K, Hayashi T, Gomes TA.
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Journal Title
Int J Med Microbiol.
Volume: 27
Issue: 3
Pages: 152-164
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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