| Project/Area Number |
25670473
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | University of Yamanashi |
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Principal Investigator |
KUBOTA Takeo 山梨大学, 総合研究部, 教授 (70293511)
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| Co-Investigator(Kenkyū-buntansha) |
TOYODA Atsushi 国立遺伝学研究所, 生物遺伝資源情報総合センター, 特任准教授 (10267495)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | エピジェネティクス / エピゲノム / 治療薬 / iPS細胞 / レット症候群 / 自閉症 / 遺伝子発現制御 / バルプロ酸ナトリウム / 遺伝子発現 / てんかん |
|---|
| Outline of Final Research Achievements |
We investigated abnormalities in the neuronal cells differentiated from the induced pluripotent stem cells (iPSC) derived from Rett syndrome patients, a representative autistic disorder caused by MECP2 mutations. As a result, we found abnormal expression of astrocyte-specific genes. Furthermore, we found that the chemicals, target to HDAC and Sin3 which are parts of MeCP2 protein complex, up-regulated Rett syndrome- and autism-associated genes. These findings suggest that malfunction of MeCP2-induced genomic changes may underlie the alteration of astrocyte-specific genes, and indicate that the chamicals examined possibly recover the gene expression which were down-regulated in Rett and autistic patients.
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