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Establishment of mouse model of chronic mucocutaneous candidiasis disease and use them to find therapeutic target

Research Project

Project/Area Number 25670477
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pediatrics
Research InstitutionHiroshima University

Principal Investigator

OKADA Satoshi  広島大学, 大学病院, 病院助教 (80457241)

Co-Investigator(Kenkyū-buntansha) TSUMURA Miyuki  広島大学, 医師薬保健学研究院, 研究員 (80646274)
Co-Investigator(Renkei-kenkyūsha) OHARA Osamu  公益財団法人かずさDNA研究所, ヒトゲノム研究部, 研究室長 (20370926)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsSTAT1 / CMCD / IL-17 / マウス / ノックインマウス / 治療
Outline of Final Research Achievements

We clarified the impact of STAT1 GOF mutations on the development of IL-17 producing T cells. We first investigated the effect of IFN-a/b, IFN-g and IL-27, cytokines that predominantly activate STAT1, in the differentiation of naive T cells into IL-17A-producing cells. We observed a much stronger reduction of the proportion of IL-17A T cells in the patients than in healthy controls. These data suggested that the poor development of IL-17 T cells in patients heterozygous for STAT1 alleles could result from enhanced IFNs and IL-27 responses via STAT1. We then treated cells with neutralizing antibodies (Abs) against IFN-a/b, IFN-g and IL-27. The combination of these Abs rescued the development of IL-17 T cells in CMCD patients carrying STAT1 mutations. These experiments established a key mechanism underlying impaired development of IL-17 T cells in the patients. This discovery also give us a new potential therapeutic target to cure host susceptibility to Candida in those patients.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] DOCK2 and a Recessive Immunodeficiency with Early-Onset Invasive Infections.2015

    • Author(s)
      Dobbs K, Conde CD, Zhang SY, Parolini S, Audry M, Chou J, Haapaniemi E, Keles S, Bilic I, Okada S, et al.
    • Journal Title

      N Engl J Med

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human intracellular ISG15 prevents interferon-α/β over-amplification and auto-inflammation.2015

    • Author(s)
      Zhang X, Bogunovic D, Payelle-Brogard B, Francois-Newton V, Speer SD, Yuan C, Volpi S, Li Z, Sanal O, Mansouri D, Tezcan I, Rice GI, Chen C, Mansouri N, Mahdaviani SA, Itan Y, Boisson B, Okada S, et al.
    • Journal Title

      Nature

      Volume: 517 Issue: 7532 Pages: 667-76

    • DOI

      10.1038/nature13801

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Life-threatening influenza and impaired interferon amplification in human IRF7 deficiency2014

    • Author(s)
      Ciancanelli MJ, Huang SX, Luthra, Garner H, Itan Y, Volpi S, Lafaille FG, Trouillet C, Schmolke M, Albrecht RA, Israelsson E, Lim HK, Casadio M, Hermesh T, Lorenzo L, Leung LW, Pedergnana V, Boisson B, Okada S, et al.
    • Journal Title

      Science

      Volume: Epub Issue: 6233 Pages: 448-53

    • DOI

      10.1126/science.aaa1578

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple diagnosis of STAT1 gain-of-function alleles in patients with chronic mucocutaneous candidiasis.2014

    • Author(s)
      Mizoguchi Y, Tsumura M, Okada S, Hirata O, Minegishi S, Imai K, Hyakuna N, Muramatsu H, Kojima S, Ozaki Y, Imai T, Takeda S, Okazaki T, Ito T, Yasunaga S, Takihara Y, Bryant VL, Kong XF, Cypowyj S, Boisson-Dupuis S, Puel A, Casanova JL, Morio T, Kobayashi M.
    • Journal Title

      J Leukoc Biol.

      Volume: 95 Issue: 4 Pages: 667-76

    • DOI

      10.1038/jid.2013.480

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] New and recurrent gain-of-function STAT1 mutations in patients with chronic mucocutaneous candidiasis from Eastern and Central Europe.2013

    • Author(s)
      Soltesz B, Toth B, Shabashova N, Bondarenko A, Okada S, Cypowyj S, Abhyankar A, Csorba G, Tasko S, Sarkadi AK, Mehes L, Rozsival P, Neumann D, Chernyshova L, Tulassay Z, Puel A, Casanova JL, Sediva A, Litzman J, Marodi L.
    • Journal Title

      J Med Genet.

      Volume: 50 Issue: 9 Pages: 567-78

    • DOI

      10.1136/jmedgenet-2013-101570

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heterozygosity for the Y701C STAT1 mutation in a multiplex kindred with multifocal osteomyelitis2013

    • Author(s)
      Hirata, O.
    • Journal Title

      Haematologica

      Volume: 98 Issue: 10 Pages: 1641-1649

    • DOI

      10.3324/haematol.2013.083741

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Partial IFN-γR2 deficiency is due to protein misfolding and can be rescued by inhibitors of glycosylation.2013

    • Author(s)
      Moncada-Velez M, Martinez-Barricarte R, Bogunovic D, Kong XF, Blancas-Galicia L, Tirpan C, Aksu G, Vincent QB, Boisson B, Itan Y, Ramirez-Alejo N, Okada S, et. al.
    • Journal Title

      Blood

      Volume: 122 Issue: 14 Pages: 2390-401

    • DOI

      10.1182/blood-2013-01-480814

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel homozygous p.R1105X mutation of the AP4E1 gene in twins with hereditary spastic paraplegia and mycobacterial disease.2013

    • Author(s)
      Kong XF, Bousfiha A, Rouissi A, Itan Y, Abhyankar A, Bryant V, Okada S, Ailal F, Bustamante J, Casanova JL, Hirst J, Boisson-Dupuis S.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 3 Pages: e58286-e58286

    • DOI

      10.1371/journal.pone.0058286

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Age-dependent association between pulmonary tuberculosis and common TOX variants in the 8q12-13 linkage region.2013

    • Author(s)
      Grant AV, El Baghdadi J, Sabri A, El Azbaoui S, Alaoui-Tahiri K, Abderrahmani Rhorfi I, Gharbaoui Y, Abid A, Benkirane M, Raharimanga V, Richard V, Orlova M, Boland A, Migaud M, Okada S, et al.
    • Journal Title

      Am J Hum Genet.

      Volume: 92 Issue: 3 Pages: 407-14

    • DOI

      10.1016/j.ajhg.2013.01.013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Chronic Mucocutaneous Candidiasis2015

    • Author(s)
      Okada S
    • Organizer
      Pediatric Academic Society annual meeting 2015
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-04-25 – 2015-04-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新生児期の好中球減少症2014

    • Author(s)
      岡田 賢, 中村和洋, 小林正夫
    • Organizer
      第24回 日本産婦人科・新生児血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2014-06-13 – 2014-06-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 新生児期の好中球減少症2014

    • Author(s)
      岡田 賢
    • Organizer
      日本産婦人科・新生児血液学会学術集会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] STAT1異常による原発性免疫不全症2013

    • Author(s)
      岡田 賢
    • Organizer
      日本小児科学会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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