Clonal evolution analyses between relapse and diagnosis samples in pediatric acute myeloid leukemia by next generation sequencer

• Project/Area Number: 25670482
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Gunma Institute of Public Health and Environmental Sciences
• Principal Investigator: HAYASHI Yasuhide 群馬県衛生環境研究所, 研究企画係, 研究員 (30238133)
• Co-Investigator(Kenkyū-buntansha): OHKI Kentarou 国立成育医療研究センター, 小児血液・腫瘍研究部, 室長 (50400966) ; PARK Myoung-ja 群馬県衛生環境研究所, 研究企画係, 研究員 (50450375)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 遺伝子 / ゲノム / マイクロアレイ / 癌 / 臨床

Outline of Final Research Achievements

Whole-exome resequencing of 19 cases with acute myeloid leukemia (AML) at diagnosis and 10 cases both at diagnosis and relapse was performed with a mean coverage of approximately x100. Several genes including BCOR/BCORL1 and RAD21 were newly identified. Furthermore, we examined mutations of these genes in pediatric 192 AML patients by gene targeting. In total, 32 mutations were identified in 31 of these specimens. The mutually exclusive pattern of the mutations in these BCOR/BCORL1 and cohesin components genes was identified, however, no associations of these genes with the prognosis were found. Transcriptome analysis of AML cases with MEL1 high expression identified novel chimeric fusions, and clonal evolutions were found. Our results indicated that a subset of subclone emerged at relapse, suggesting heterogeneity in AML. Extensive methylation analysis is going to be done.

Research Products

• [Journal Article] High expression of EVI1 and MEL1 is a compelling poor prognostic marker of pediatric AML. (2015)
  • Author(s): Jo A, Mitani S, Shiba N, Hayashi Y, Hara Y, Takahashi H, Tsukimoto I, Tawa A, Horibe K, Tomizawa D, Taga T, Adachi S, Yoshida T, Ichikawa H
  • Journal Title: Leukemia Volume: 29 Issue: 5 Pages: 1067-1083
  • DOI: 10.1038/leu.2015.5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Integrated genetic and epigenetic analysis defines novel molecular subgroups in rhabdomyosarcoma. (2015)
  • Author(s): Seki M, Nishimura R, Yoshida K, Shimamura T, Shiraishi Y, Sato Y, Kato M, Chiba K, Tanaka H, Hoshino N, Nagae G, Shiozawa Y, Okuno Y, Hosoi H, Tanaka Y, Okita H, Miyachi M, Souzaki R, Taguchi T, Koh K, Hanada R, Kato K, Nomura Y, Akiyama M, Oka A, Igarashi T, Miyano S, Aburatani H, Hayashi Y, Ogawa S, Takita J.
  • Journal Title: Nat Commun. Volume: 6 Issue: 1 Pages: 7557-7557
  • DOI: 10.1038/ncomms8557
  • NAID: 120005623266
  • Peer Reviewed / Open Access
• [Journal Article] CSF3R and CALR mutations in paediatric myeloid disorders and the association of CSF3R mutations with translocations, including t(8; 21). (2015)
  • Author(s): Sano H, Ohki K, Park MJ, Shiba N, et al.
  • Journal Title: Br J Haematol. Volume: Epub ahead of print Issue: 3 Pages: 319-397
  • DOI: 10.1111/bjh.13439
  • Peer Reviewed / Open Access
• [Journal Article] Adverse prognostic impact of KIT mutations in childhood CBF-AML: the results of the Japanese Pediatric Leukemia/Lymphoma Study Group AML-05 trial. (2015)
  • Author(s): Tokumasu M, Murata C, Shimada A, Ohki K, Hayashi Y, Saito AM, Fujimoto J, Horibe K, Nagao M, Itoh H, Kamikubo Y, Nakayama H, Kinoshita A, Tomizawa D, Taga T, Tawa A, Tanaka S, Heike T, Adachi S
  • Journal Title: Leukemia Volume: 29 Issue: 12 Pages: 2438-2441
  • DOI: 10.1038/leu.2015.121
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High PRDM16 expression identifies a prognostic subgroup of pediatric acute myeloid leukaemia correlated to FLT3-ITD, KMT2A-PTD, and NUP98-NSD1: the results of the Japanese Paediatric Leukaemia/Lymphoma Study Group AML-05 trial. (2015)
  • Author(s): Shiba N, Ohki K, Kobayashi T, Hara Y, Yamato G, Tanoshima R, Ichikawa H, Tomizawa D, Park MJ, Shimada A, Sotomatsu M, Arakawa H, Horibe K, Adachi S, Taga T, Tawa A, Hayashi Y.
  • Journal Title: Br J Haematol. Volume: 172 Issue: 4 Pages: 581-91
  • DOI: 10.1111/bjh.13869
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gene alterations involving the CRLF2-JAK pathway and recurrent gene deletions in Down syndrome-associated acute lymphoblastic leukemia in Japan. (2014)
  • Author(s): Hanada I, Terui K, Ikeda F, Toki T, Kanezaki R, Sato T, Kamio T, Kudo K, Sasaki S, Takahashi Y, Hayashi Y, Inukai T, Kojima S, Koike K, Kosaka Y, Kobayashi M, Imaizumi M, Mitsui T, Hori H, Hara J, Horibe K, Nagai J, Goto H, Ito E.
  • Journal Title: Genes Chromosomes Cancer Volume: 53 Issue: 11 Pages: 902-910
  • DOI: 10.1002/gcc.22201
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic impact of gained chromosomes in high-hyperdiploid childhood acute lymphoblastic leukaemia (2014)
  • Author(s): Kato M, Imamura T, Manabe A, Hashii Y, Koh K, Sato A, Takahashi H, Hori H, Taki T, Inoue M, Hayashi Y, Horibe K, Tsuchida M, Kojima S, Oda M, Ohara A .
  • Journal Title: Br J Haematol Volume: 166 Issue: 2 Pages: 295-298
  • DOI: 10.1111/bjh.12836
  • Peer Reviewed
• [Journal Article] Acute myeloid leukaemia with myelodysplastic features in children (2014)
  • Author(s): Kinoshita A, Miyachi H, Matsushita H, Yabe M, Taki T, Watanabe T, Saito AM, Tomizawa D, Taga T, Takahashi H, Matsuo H, Kodama K, Ohki K, Hayashi Y, Tawa A, Horibe K, Adachi S.
  • Journal Title: Br J Haematol Volume: 167 Issue: 1 Pages: 80-86
  • DOI: 10.1111/bjh.12993
  • Peer Reviewed
• [Journal Article] EVI1 overexpression is a poor prognostic factor in pediatric patients with mixed lineage leukemia-AF9 rearranged acute myeloid leukemia. (2014)
  • Author(s): Matsuo H, Kajihara M, Tomizawa D, Watanabe T, Saito AM, Fujimoto J, Horibe K, Kodama K, Tokumasu M, Itoh H, Nakayama H, Kinoshita A, Taga T, Tawa A, Taki T, Shiba N, Ohki K, Hayashi Y, Yamashita Y, Shimada A, Tanaka S, Adachi S.
  • Journal Title: Haematologica Volume: 99 Issue: 11 Pages: 225-227
  • DOI: 10.3324/haematol.2014.107128
  • Peer Reviewed / Open Access
• [Journal Article] Outcome of children with relapsed acute myeloid leukemia following initial therapy under the AML99 protocol (2014)
  • Author(s): Nakayama H, Tabuchi K, Tawa A, Tsukimoto I, Tsuchida M, Morimoto A, Yabe H, Horibe K, Hanada R, Imaizumi M, Hayashi Y, Hamamoto K, Kobayashi R, Kudo K, Shimada A, Miyamura T, Moritake H, Tomizawa D, Taga T, Adachi S.
  • Journal Title: Int J Hematol Volume: 100(2) Issue: 2 Pages: 171-9
  • DOI: 10.1007/s12185-014-1616-9
  • Peer Reviewed / Open Access
• [Journal Article] Mutations of the GATA2 and CEBPA genes in paediatric acute myeloid leukaemia. (2014)
  • Author(s): Shiba N, Funato M, Ohki K, Park MJ, Mizushima Y, Adachi S, Kobayashi M, Kinoshita A, Sotomatsu M, Arakawa H, Tawa A, Horibe K, Tsukimoto I, Hayashi Y.
  • Journal Title: Brit J Haematol Volume: 印刷中 Issue: 1 Pages: 142-159
  • DOI: 10.1111/bjh.12559
  • Peer Reviewed
• [Journal Article] Recurrent CDC25C mutations drive malignant transformation in FPD/AML (2014)
  • Author(s): Yoshimi A, Toya T, Kawazu M, Ueno T, Tsukamoto A, Iizuka H, Nakagawa M, Nannya Y, Arai S, Harada H, Usuki K, Hayashi Y, Ito E, Kirito K, Nakajima H, Ichikawa M, Mano H, Kurokawa M
  • Journal Title: Nature communications Volume: 5 Issue: 1 Pages: 4770-4770
  • DOI: 10.1038/ncomms5770
  • Peer Reviewed / Open Access
• [Journal Article] Liver disease is frequently observed in Down syndrome patients with transient abnormal myelopoiesis. (2014)
  • Author(s): Park MJ, Sotomatsu M, Ohki K, Arai K, Maruyama K, Kobayashi T, Nishi A, Sameshima K, Takagi T, Hayashi Y.
  • Journal Title: Int J Hematol Volume: 99 Issue: 2 Pages: 154-161
  • DOI: 10.1007/s12185-013-1487-5
  • Peer Reviewed
• [Journal Article] SETBP1 mutations in juvenile myelomonocytic leukaemia and myelodysplastic syndrome but not in paediatric acute myeloid leukaemia (2013)
  • Author(s): Shiba N, Ohki K, Park MJ, Sotomatsu M, Kudo K, Ito E, Sako M, Arakawa H, Hayashi Y
  • Journal Title: Br J Haematol Volume: Epub ahead of print Issue: 1 Pages: 156-159
  • DOI: 10.1111/bjh.12595
  • Peer Reviewed
• [Journal Article] The landscape of somatic mutations in Down syndrome-related myeloid disorders. (2013)
  • Author(s): Yoshida K
  • Journal Title: Nature Genet. Volume: 45 Issue: 11 Pages: 1293-1200
  • DOI: 10.1038/ng.2759
  • Peer Reviewed
• [Journal Article] WT1 mutation in pediatric patients with acute myeloid leukemia: A study of the Japanese Childhood AML Cooperative Study Group (2013)
  • Author(s): Sano H, Shimada A, Tabuchi K, Taki T, Murata C, Park M, Sotomatsu Tawa A, Kobayashi R, Horibe K, Tsuchida M, Hanada R, Tsukimoto I, Hayashi Y
  • Journal Title: Int J Hematol Volume: 98 Issue: 4 Pages: 437-445
  • DOI: 10.1007/s12185-013-1409-6
  • Peer Reviewed
• [Journal Article] An empirical Bayesian framework for somatic mutation detection from cancer genome sequencing data (2013)
  • Author(s): Yuichi Shiraishi
  • Journal Title: Nucleic Acid Research Volume: 41 Issue: 7 Pages: e89-e89
  • DOI: 10.1093/nar/gkt126
  • Peer Reviewed
• [Journal Article] Naturally occurring oncogenic GATA1 mutants with internal deletions in transient abnormal myelopoiesis in Down syndrome (2013)
  • Author(s): Toki T, Kanezaki R, Kobayashi E, Kaneko H, Suzuki M, Wang R, Terui K, Kanegane H, Maeda M, Endo M, Mizuochi T, Adachi S, Hayashi Y, Yamamoto M, Shimizu R, Ito E.
  • Journal Title: Blood Volume: 121(16) Issue: 16 Pages: 3181-3184
  • DOI: 10.1182/blood-2012-01-405746
  • Peer Reviewed
• [Presentation] A Combination of EVI1 and PRDM16 Expression Clarified the Clinical Features of Intermediate/High Risk Patients in Pediatric Acute Myeloid Leukemia (2015)
  • Author(s): Yamato G, Shiba N, Hara Y, Park M, Sotomatsu M, Ohki K, Ichikawa H, Tomizawa D, Taga T, Horibe K, Tawa A, Arakawa H, Adachi S, Hayashi Y
  • Organizer: 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orland
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] The Outcome of Relapsed Childhood Core Binding Factor Acute Myeloid Leukemia: A Report from the JPLSG AML-05R Study (2015)
  • Author(s): Moritake H, Tanaka S, Nakayama H, Miyamura T, Iwamoto S, Shimada A, Terui K, Saito A, Shiba N, Hayashi Y, Tomizawa D, Taga T, Goto H, Manabe A, Horibe K, Mizutani S, Adachi S
  • Organizer: 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orland
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] Detection of Novel Pathogenic Gene Rearrangements in Pediatric Acute Myeloid Leukemia By RNA Sequencing (2015)
  • Author(s): Shiba N, Yoshida K, Shiraishi Y, Hara Y, Yamato G, Kaburaki T, Sanada M, Oki K, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Park M, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y
  • Organizer: 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orland
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] High BMP2 Expression Is a Poor Prognostic Factor and a Good Candidate to Identify CBFA2T3-GLIS2-like High-Risk Subgroup in Pediatric Acute Myeloid Leukemia (2015)
  • Author(s): Hara Y, Yamato G, Shiba N, Ohki K, Park M, Tomizawa D, Taki T, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y
  • Organizer: 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orland
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] RNA seqにより見いだされた新規遺伝子の解析と臨床像 (2015)
  • Author(s): 原 勇介, 柴 徳生, 大和 玄季, 大木 健太郎, 朴 明子, 外松 学, 滝 智彦, 吉田 健一, 眞田 昌, 富澤 大輔, 荒川 浩一, 堀部 敬三, 多賀 崇, 足立 壮一, 多和 昭雄, 宮野 悟, 小川 誠司, 林 泰秀
  • Organizer: 第57回日本小児血液・がん学会学術総会
  • Place of Presentation: 甲府
  • Year and Date: 2015-11-27
• [Presentation] 小児急性骨髄性白血病におけるPRDM16遺伝子とEVI1遺伝子発現の臨床的意義 (2015)
  • Author(s): 大和 玄季, 柴 徳生, 原 勇介, 朴 明子, 外松 学, 大木 健太郎, 市川 仁, 富澤 大輔, 多賀 崇, 堀部 敬三, 多和 昭雄, 荒川 浩一, 足立 壮一, 林 泰秀
  • Organizer: 第57回日本小児血液・がん学会学術総会
  • Place of Presentation: 甲府
  • Year and Date: 2015-11-27
• [Presentation] The prognostic impact of PRDM16 gene expression in both adult and pediatric acute myeloid leukemia (2015)
  • Author(s): Yamato G, Yamaguchi H, Handa H, Shiba N, Wakita S, Inokuchi K, Hara Y, Ohki K, Park M, Sotomatsu M, Ichikawa H, Tomizawa D, Taga T, Adachi S, Horibe K, Tawa A, Arakawa H, Hayashi Y
  • Organizer: 第77回日本血液学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-10-16
• [Presentation] RNA sequencing reveals the comprehensive gene alterations of pediatric acute myeloid leukemia (2015)
  • Author(s): Shiba N, Yoshida K, Sanada M, Shiraishi Y, Hara Y, Yamato G, Ohki K, Park M, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y
  • Organizer: 第77回日本血液学会学術総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-10-16
• [Presentation] 家族性骨髄異形成症候群とその原因候補遺伝子 (2015)
  • Author(s): 高岡 賢輔, 河津 正人, 吉見 昭秀, 遠矢 嵩, 小林 隆, 南谷 泰仁, 上野 博則, 原田 浩徳, 林 泰秀, 間野 博行, 黒川 峰夫
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] 中間リスク、高リスク小児急性骨髄性白血病における新たな治療層別化の試み (2015)
  • Author(s): 柴 徳生, 原 勇介, 大木 健太郎, 大和 玄季, 富澤 大輔, 多賀 崇, 多和 昭雄, 荒川 浩一, 足立 壮一, 林 泰秀, JPLSG AML委員会
  • Organizer: 第118回日本小児科学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-04-17
• [Presentation] Whole Exome and Transcriptome Analyses in Pediatric T-Cell Acute Lymphoblastic Leukemia. (2014)
  • Author(s): Seki M, Yoshida K, Shiraishi Y, Chiba K, Tanaka H, Kato M, Hanada R, Miyano S, Hayashi Y, Ogawa S, Takita J.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2014-12-08
• [Presentation] The Prognostic Impact of High MEL1 Gene Expression in Pediatric Acute Myeloid Leukemia. (2014)
  • Author(s): Shiba N, Ohki K, Hara Y, Yamato G, Park M, Ichikawa H, Kobayashi T, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Taga T, Adachi S, Tawa A, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2014-12-06
• [Presentation] Poor Prognosis Associated with FAB Subtypes M4 and M5 in Japanese Pediatric Acute Myeloid Leukemia Patients with FLT3-ITD. (2014)
  • Author(s): Hara Y, Shiba N, Ohki K, Park M, Shimada A, Tomizawa D, Moriya Saito M, Fujimoto J, Taki T, Kinoshita A, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2014-12-06
• [Presentation] Clinical Features of Patients with ASXL1 and ASXL2 Mutations in Pediatric Acute Myeloid Leukemia. (2014)
  • Author(s): Yamato G, Shiba N, Yoshida K, Ohki K, Park M, Hara Y, Tomizawa D, Sotomatsu M, Taga T, Adachi S, Tawa A, Horibe K, Arakawa H, Ogawa S, Hayashi Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2014-12-06
• [Presentation] 小児AMLにおけるIKZF1欠失の頻度と予後解析 (2014)
  • Author(s): 大木 健太郎, 朴 明子, 原 勇介, 柴 徳生, 外松 学, 富澤 大輔, 多賀 崇, 齋藤 明子, 藤本 純一郎, 多和 昭雄, 堀部 敬三, 足立 壮一, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-29
• [Presentation] 小児急性骨髄性白血病における寛解導入療法後非寛解例の遺伝子解析による予後不良因子の同定 (2014)
  • Author(s): 原 勇介, 大木 健太郎, 柴 徳生, 朴 明子, 富澤 大輔, 多賀 崇, 荒川 浩一, 足立 壮一, 多和 昭雄, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-29
• [Presentation] 小児急性骨髄性白血病におけるASXL1、ASXL2遺伝子変異と臨床像 (2014)
  • Author(s): 大和 玄季, 柴 徳生, 吉田 健一, 大木 健太郎, 朴 明子, 原 勇介, 外松 学, 多賀 崇, 富澤 大輔, 足立 壮一, 多和 昭雄, 堀部 敬三, 荒川 浩一, 小川 誠司, 林 泰秀.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-29
• [Presentation] The prognostic impact of high EV1-related genes expression in pediatric acute myeloid leukemia. (2014)
  • Author(s): Shiba N, Hara Y, Ohki K, Yamato G, Park M, Kobayashi T, Ichikawa H, Tomizawa D, Taki T, Shimada A, Sotomatsu M, Arakawa H, Horibe H, Adachi S, Tawa A, Hayashi Y.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-02
• [Presentation] CSF3R and CALR mutations and cytogenetic findings in pediatric myeloid malignancies. (2014)
  • Author(s): Sano H, Ohki K, Park M, Shiba N, Hara Y, Sotomatsu M, Tomizawa D, Taga T, Kiyokawa N, Tawa A, Horibe K, Adachi A, Hayashi Y.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-01
• [Presentation] 小児急性骨髄性白血病における分子生物学的背景を用いた新たな治療層別化への試み. (2014)
  • Author(s): 柴 徳生, 吉田 健一, 大木 健太郎, 金澤 崇, 足立 壮一, 多和 昭雄, 伊藤 悦朗, 荒川 浩一, 小川 誠司, 林 泰秀, JPLSG AML委員会
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-13
• [Presentation] 小児急性骨髄性白血病におけるGATA2変異の解析. (2014)
  • Author(s): 原 勇介, 柴 徳生, 大木 健太郎, 朴 明子, 足立 壮一, 多賀 崇, 荒川 浩一, 多和 昭雄, 堀部 敬三, 林 泰秀.
  • Organizer: 第117回日本小児科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-12
• [Presentation] Recurrent CDC25C mutations drive malignant transformation in FPD/AML. (2014)
  • Author(s): Yoshimi A, Toya T, Kawazu M, Ueno T, Tsukamoto A, Iizuka1 H, Nakagawa M, Nannya Y, Arai S, Ichikawa M, Harada H, Usuki K, Hayashi Y, Ito E, Kirito K, Nakajima H, Mano H, Kurokawa M.
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: San Diego, U.S.A.
  • Year and Date: 2014-04-08 – 2014-04-09
• [Presentation] Whole-Exome Resequencing Identifies Somatic Mutations Of BCOR and BCORL1 Transcriptional Corepressor Genes and Major Cohesin Complex Component Genes In Pediatric Acute Myeloid Leukemia. (2013)
  • Author(s): Shiba N, Ohki K, Nagata Y, Kon A, Okuno Y,Shiraishi Y, Kato M, Park MJ, Ohki K, Takita J, Kanazawa T, Kudo K, Ito E, Sanada M, Tomizawa D, Tawa A, Adachi S, Miyano S, Ogawa S, Hayashi Y.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] Low Frequency and Poor Prognosis Of MLL-Partial Tandem Duplications In Pediatric Acute Myeloid Leukemia Using MLPA Method: The Japanese Pediatric Leukemia/Lymphoma Study Group (JPLSG) AML-05 Trial. (2013)
  • Author(s): Ohki K, Park MJ, Sano H, Hara Y, Shiba N, Tomizawa D, Taga T, Moriya Saito A, Fujimoto J, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] Whole Exome Sequencing Reveals Clonal Evolution Pattern and Driver Mutations Of Relapsed Pediatric AML. (2013)
  • Author(s): Yoshida K, Shiba N, Shiraishi Y, Shimada A, Terui K, Kato M, Okuno Y, Nagata Y, Kon A, Yoshizato T, Matsunawa, M, Chiba K, Tanaka H, Sanada M, Miyano S, Ito E, Hayashi Y, Ogawa S.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] Poor Prognosis With Different Induction Rate Was Observed In Children With Acute Myeloid Leukemia and FLT3-ITD According To The ITD/WT Allelic Ratio: A Result From The Japanese Pediatric Leukemia/Lymphoma Study Group. (2013)
  • Author(s): Shimada A, Yamashita Y, Tomizawa D, Tawa A, Watanabe T, Yokozawa T, Kudo K, Taga T, Iwamoto S, Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Koh K, Goto H, Kosaka Y, Moriya Saito A, Fujimoto J, Horibe K, Oki K, Hayashi Y, SAdachi S.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] Comprehensive Fusion Gene Analysis Of Pediatric Non-Down Syndrome Acute Megakaryoblasitc Leukemia. (2013)
  • Author(s): Hara Y, Shiba n, Ohki K, Park MJ, Tomizawa D, Taga T, Saito A, Fujimoto J, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] Genetic Landscapes Of Childhood T-Cell Acute Lymphoblastic Leukemia. (2013)
  • Author(s): Seki M, Yoshida K, Sato Y, Shiraishi Y, Chiba K, TanakaH, Kato M, Hiwatari M, Koh K, Hanada R, Sanada M, Miyano S, Oka A, Hayashi Y, Ogawa S, Takita J.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] The Genetic Landscape Of FPD/AML Revealed CDC25C Mutation As a Driver That Promotes Malignant Transformation. (2013)
  • Author(s): Yoshimi A, Toya T, Nakagawa M, Kawazu M, Nannya Y, Ichikawa M, Arai S, Harada H, Usuki K, Hayashi Y, Ito E, Kirito K, Nakajima H, Mano H, Kurokawa M.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] CSF3R Gene Mutations In Myeloid Malignancy Of Childhood. (2013)
  • Author(s): Sano H, Ohki K, Park MJ, Hara Y, Shiba N, Tomizawa D, Taga T, Moriya Saito A, Fujimoto J, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 55rd Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] 小児急性骨髄性白血病におけるGATA2遺伝子変異の解析.
  • Author(s): 柴 徳生, 朴 明子, 舩戸道徳, 小林正夫, 木下明俊, 足立壮一, 荒川浩一, 多和昭雄, 月本一郎, 林 泰秀.
  • Organizer: 小児急性骨髄性白血病におけるGATA2遺伝子変異の解析.
  • Place of Presentation: 広島
• [Presentation] NUP98-NSD1融合遺伝子陽性例は小児急性骨髄性白血病において予後不良である
  • Author(s): 原 勇介, 柴 徳生, 嶋田 明, 工藤寿子, 富澤大輔, 多賀 崇, 多和昭雄, 荒川浩一, 足立壮一, 林 泰秀.
  • Organizer: 小児急性骨髄性白血病におけるGATA2遺伝子変異の解析.
  • Place of Presentation: 広島
• [Presentation] 小児B型前駆細胞型ALLにおけるCRLF2高発現例の特徴：TCCSG-ALL研究.
  • Author(s): 大木健太郎, 朴 明子, 柴 徳生, 清河信敬, 康 勝好, 花田良二, 真部 淳, 菊地 陽, 小原 明, 林 泰秀.
  • Organizer: 小児急性骨髄性白血病におけるGATA2遺伝子変異の解析.
  • Place of Presentation: 広島
• [Presentation] 染色体異常を伴ったダウン症合併TAMとAMKLの遺伝子解析.
  • Author(s): 朴 明子, 大木健太郎, 新井 心, 外松 学, 林 泰秀.
  • Organizer: 小児急性骨髄性白血病におけるGATA2遺伝子変異の解析.
  • Place of Presentation: 広島
• [Presentation] NUP98-MSD1 gene fusion is a strong poor prognostic factor in pediatric AML.
  • Author(s): Hara Y, Shiba N, Shimada A, Kudo K, Tomizawa D, Taga T, Horibe K, Adachi S, Arakawa H, Tawa A, Hayashi Y.
  • Organizer: 45th Congress of the International Society of Pediatric Oncology.
  • Place of Presentation: Hong Kong
• [Presentation] RNAシークエンシングによる小児AMLの融合遺伝子探索.
  • Author(s): 三谷幸代, 坂本裕美, 柴 徳生, 林 泰秀, 吉田輝彦, 市川 仁.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 小児non-Down急性巨核芽球性白血病における遺伝子解析.
  • Author(s): 原 勇介, 柴 徳生, 大木健太郎, 朴 明子, 富澤大輔, 多賀 崇, 足立壮一, 荒川浩一, 多和昭雄, 堀部敬三, 林 泰秀.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] JPLSG AML05臨床試験登録症例においてMLPA法によるMLL-PTDの頻度はこれまでの報告より少なく予後不良である.
  • Author(s): 大木健太郎, 柴 徳生, 朴 明子, 富澤大輔, 多賀 崇, 堀部敬三, 多和昭雄, 足立壮一, 林 泰秀.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] A diversity of cases in AML with promyelocytic differentiation; A report from JPLSG.
  • Author(s): Takahashi H, Matsushita H, Kinoshita A, Taki T, Taki T, Deguchi T, Kiyokawa N, Hashii Y, Hayashi Y, Tomizawa D, Taga T, Tawa A, Adachi S, Tabe M, Miyachi H.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] Prognostic impact of KIT mutatkon in t(8;21) childhood AML: The JPLSG AML-05 trial.
  • Author(s): Tokumasu M, Nagao M, Shimada A, Murata C, Ohki K, Hayashi Y, Saito A, Fujimoto J, Horibe K, Ito H, Nakayama H, Kinoshita A, Tomizawa D, Taga T, Yamaguchi H, Tawa A, Heike T, Adachi S.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] NUP98-NSD1 gene fusion is a strong poor prognostic factor in pediatric AML.
  • Author(s): Hara Y, Shiba N, Ichikawa H, Taki T, Shimada A, Kudo K, Tomizawa D, Taga T, Adachi S, Arakawa H, Tawa A, Hayashi Y.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] Clonal architecture in a case of acute myeloid leukemia with trisomy 8 and MLL-AF9.
  • Author(s): Ohki K, Kama Y, Shiba N, Arai K, Park MJ, Sotomatsu M, Hayashi Y.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] Genetic basisi of myeloid leukemogenesisi in Down syndrome.
  • Author(s): Yoshida K, Toki T, Okuno Y, Kanezaki R, Shiraishi Y, Sanada M, Park MJ, Terui K, Kon A, Nagata Y, Sato-Otsubo A, Sato Y, Wang R, Shiba N, Chiba K, Tanaka H, Hama A, Muramatsu H, Hasegawa D, Nakamura K, Kanegane H, Tsukamoto K, Adachi S, Kawakami K, Hayashi Y, Miyano S, Kojima S, Ito E, Ogawa S.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] 13q欠失を伴ったダウン症合併TAMとAMKLの遺伝子解析.
  • Author(s): 朴 明子, 吉田健一, 大木健太郎, 新井 心, 外松 学, 伊藤悦朗, 小川誠司, 林 泰秀.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] Gata2 mutations are found in pediatric AML but not in other leukemias including JMML.
  • Author(s): Hara Y, Shiba N, Funato M, Oki K, Park MJ, Mizushima Y, Adachi S, Kobayashi M, Kinoshita A, Sotomatsu M, Arakawa H, Tawa A, Horibe K, Tsukimoto I, Hayashi Y.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] Whole-exome resequencing reveals novel pathogenetic gene mutations in pediatric AML.
  • Author(s): Shiba N, Yoshida K, Okuno Y, Shiraishi Y, Nagata Y, Kon A, Chiba K, Tanaka H, Ohki K, Kato M, Terui K, Park MJ, Kanazawa T, Takita J, Kudo K, Arakawa H, Ito E, Sanada M, Miyano S, Ogawa S, Hayashi Y.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] Recurrent SETBP1 mutation in juvenile myelomonocytic leukemia and myelodysplastic syndrome.
  • Author(s): Shiba N, Hara Y, Park MJ, Ohki K, Fukushima K, Sako M, Kudo K, Arakawa H, Ito E, Hayashi Y.
  • Organizer: The 75th Annual Meeting of the Japanese Society of Hematolgy.
  • Place of Presentation: 札幌
• [Presentation] 小児血液・腫瘍の染色体・分子遺伝学入門とその臨床応用.
  • Author(s): 林 泰秀
  • Organizer: 第55回日本小児血液・がん学会学術集会(meet the expert)
  • Place of Presentation: 福岡
• [Presentation] 全エクソーム解析による小児急性骨髄性白血病の新規原因遺伝子の同定.
  • Author(s): 柴 徳生, 林 泰秀.
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 小児白血病におけるSETBP1遺伝子変異の解析.
  • Author(s): 柴 徳生, 大木健太郎, 朴 明子, 工藤寿子, 福島啓太郎, 伊藤悦朗, 迫 正廣, 多和昭雄, 荒川浩一, 林 泰秀.
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 急性骨髄性白血病におけるWT1遺伝子変異の解析.
  • Author(s): 佐野弘純, 嶋田 明, 田渕 健, 滝 智彦, 村田知里, 朴 明子, 大木健太郎, 外松 学, 足立壮一, 多和昭雄, 小林良二, 堀部敬三, 土田昌宏, 花田良二, 月本一郎, 林 泰秀.
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 全エクソーム解析による肝芽腫における網羅的ゲノム解析.
  • Author(s): 星野諭子, 西村 力, 関 正史, 奥野友介, 吉田健一, 白石友一, 加藤元博, 宮野 悟, 岡 明, 林 泰秀, 岩中 督, 小川誠司, 滝田順子.
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 散発性胸膜肺芽腫におけるDICER1の両アレル変異.
  • Author(s): 関 正史, 吉田健一, 白石友一, 佐藤悠佑, 西村 力, 奥野友介, 千葉健一, 田中陽子, 加藤啓輔, 加藤元博, 花田良二, 野村優子, 朴 明子, 石田敏章, 岡 明, 宮野 悟, 林 泰秀, 小川誠司, 滝田順子.
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 小児骨髄造血器腫瘍におけるCSF3R遺伝子異常の解析.
  • Author(s): 佐野仁志, 大木健太郎, 朴 明子, 柴 徳生, 原 勇介, 外松 学, 足立壮一, 堀部敬三, 多和昭雄, 花田良二, 月本一郎, 林 泰秀.
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: 福岡