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Investigation of the mechanisms and development of new therapies for lung hypoplasia

Research Project

Project/Area Number 25670493
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Embryonic/Neonatal medicine
Research InstitutionNational Center for Child Health and Development

Principal Investigator

Fujinaga Hideshi  国立研究開発法人国立成育医療研究センター, その他部局等, その他 (60623733)

Co-Investigator(Renkei-kenkyūsha) Umezawa Akihiro  独立行政法人国立成育医療研究センター, 再生医療センター, センター長 (70213486)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords肺低形成 / 血管内皮前駆細胞 / ECFC / VEGF / SDF1 / 血管内皮成長因子
Outline of Final Research Achievements

Vascular growth is necessary for normal lung development. We hypothesized that the function of endothelial colony-forming cells (ECFCs), a type of endothelial progenitor cells (EPCs), is impaired in congenital diaphragmatic hernia (CDH), that is associated with lung hypoplasia. Cord blood (CB) was collected from CDH patients and healthy controls. We assessed CB progenitor cell populations and CB ECFC functions. CB ECFCs were decreased in CDH. CDH ECFCs had reduced potential for self-renewal, proliferation and migration. Their capacity for nitric oxide (NO) production was enhanced but response to VEGF was blunted. The in vivo potential for vasculogenesis was reduced in CDH ECFCs. There was no difference in VEGF and SDF1α levels in CB plasma and culture media, and ECFC mRNA expression associated with VEGF-NO and SDF1-CXCR4 signaling between groups. In conclusion, CB ECFC function is disrupted in CDH, probably due to mechanisms other than alteration of VEGF-NO and SDF1-CXCR4 signaling.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2016 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Cord Blood-Derived Endothelial Colony-Forming Cell Function is Disrupted in Congenital Diaphragmatic Hernia2016

    • Author(s)
      Hideshi Fujinaga, Hiroko Fujinaga, Nobuyuki Watanabe, Tomoko Kato, Moe Tamano, Miho Terao, Shuji Takada, Yushi Ito, Akihiro Umezawa, Masahiko Kuroda
    • Journal Title

      American Journal of Physiology-Lung Cellular and Molecular Physiology

      Volume: 310 Issue: 11 Pages: 1143-1154

    • DOI

      10.1152/ajplung.00357.2015

    • NAID

      120005845870

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Cord Blood-Derived Endothelial Colony-Forming Cell Function is Disrupted in Congenital Diaphragmatic Hernia2016

    • Author(s)
      Hideshi Fujinaga, Hiroko Fujinaga, Yushi Ito, Akihiro Umezawa, Masahiko Kuroda
    • Organizer
      American Thoracic Society 2016 international conference
    • Place of Presentation
      San Francisco
    • Year and Date
      2016-05-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 肺低形成関連疾患に対するNO吸入療法の有用性2014

    • Author(s)
      藤永英志
    • Organizer
      新生児iNO教育セミナー
    • Place of Presentation
      コンファレンススクエアM+
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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