| Project/Area Number |
25670501
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSAKI Michiya 大阪大学, 工学研究科, 助教 (00419467)
AKASHI Mitsuru 大阪大学, 工学研究科, 教授 (20145460)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | リンパ管新生 / シグナル伝達 / 増殖因子 / 細胞 / 癌 / 細胞培養 / 細胞外マトリックス / 再生医療 |
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| Outline of Final Research Achievements |
Cultured lymphatic endothelial cells (LEC) show marked response to recombinant VEGF-A in a physiological condition, whereas LEC show a minimum response to highly-concentrated recombinant VEGF-C in mono-layered culture condition. Therefore, we decided to generate a novel three-dimensional culture system that gives rise to the biological effect of recombinant VEGF-C to cultured LEC. Cultured LEC were seeded on the fibroblasts, and incubated for 48 hours in the presence or absence of recombinant VEGF-C at 50 ng/ml. The three dimensional tissues showed that cultured LEC develop highly organized networks in the presence of VEGF-C as compared with controls. Our results indicate, for the first time, that physiological lymphatic vessel growth is induced by VEGF-C in the three dimensional tissue, and that our novel culture system may clarify the biological mechanism of lymphangiogenesis in physiological and pathological condition.
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