• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biological effect of VEGF-C on cultured lymphatic endothelial cells

Research Project

Project/Area Number 25670501
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

HIRAKAWA Satoshi  浜松医科大学, 医学部, 准教授 (50419511)

Co-Investigator(Renkei-kenkyūsha) MATSUSAKI Michiya  大阪大学, 工学研究科, 助教 (00419467)
AKASHI Mitsuru  大阪大学, 工学研究科, 教授 (20145460)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsリンパ管新生 / シグナル伝達 / 増殖因子 / 細胞 / 癌 / 細胞培養 / 細胞外マトリックス / 再生医療
Outline of Final Research Achievements

Cultured lymphatic endothelial cells (LEC) show marked response to recombinant VEGF-A in a physiological condition, whereas LEC show a minimum response to highly-concentrated recombinant VEGF-C in mono-layered culture condition. Therefore, we decided to generate a novel three-dimensional culture system that gives rise to the biological effect of recombinant VEGF-C to cultured LEC. Cultured LEC were seeded on the fibroblasts, and incubated for 48 hours in the presence or absence of recombinant VEGF-C at 50 ng/ml. The three dimensional tissues showed that cultured LEC develop highly organized networks in the presence of VEGF-C as compared with controls. Our results indicate, for the first time, that physiological lymphatic vessel growth is induced by VEGF-C in the three dimensional tissue, and that our novel culture system may clarify the biological mechanism of lymphangiogenesis in physiological and pathological condition.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (2 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Development of full-thickness human skin equivalens with blood and lymph-like capillary networks by cell coating technology2015

    • Author(s)
      Matsusaki M, Fujimoto K, Shirakata Y, Hirakawa S, Hashimoto K, Akashi M.
    • Journal Title

      J Biomed Mater Res A

      Volume: 印刷中 Issue: 10 Pages: 3386-3396

    • DOI

      10.1002/jbm.a.35473

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lymphatics in nanophysiology2014

    • Author(s)
      Hirakawa S, Detmar M, Karaman S.
    • Journal Title

      Advanced Drug Delivery Reviews

      Volume: 印刷中 Pages: 12-18

    • DOI

      10.1016/j.addr.2014.01.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 血管リンパ管に着目した再生医療と応用2013

    • Author(s)
      平川聡史
    • Journal Title

      日本皮膚科学会誌

      Volume: 123 Pages: 2405-2406

    • Related Report
      2013 Research-status Report
  • [Presentation] 三次元培養:癌リンパ行性転移を評価する実験系の構築2014

    • Author(s)
      平川聡史
    • Organizer
      第38回日本リンパ学会総会
    • Place of Presentation
      東京
    • Year and Date
      2014-06-20 – 2014-06-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Three-dimensional multilayered tissue: a moderu to assess the biological effect of vascular endothelial growth factors upon cultured lymphatic endothelial cells2014

    • Author(s)
      Hirakawa S, Nishiguchi A, Matsusaki M, Akashi M.
    • Organizer
      Gordon Research Conference, Molecular mechanisms in lymphatic function and disease
    • Place of Presentation
      Lucca, Italy
    • Related Report
      2013 Research-status Report
  • [Book] 薬疹 分子標的薬を中心とした皮膚障害-診断と治療の手引き-2014

    • Author(s)
      平川聡史、青島正浩
    • Total Pages
      4
    • Publisher
      メディカルレビュー社
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi