Development of noninvasive imaging techniques in islet transplantation
Project/Area Number |
25670531
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Radiation science
|
Research Institution | Kyoto University |
Principal Investigator |
KIMURA Hiroyuki 京都大学, 放射性同位元素総合センター, 助教 (50437240)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 膵島移植定量化 / イメージング / GLP-1受容体 / SPECT / 移植膵島量の定量化 / Exendin誘導体 / GW9508誘導体 / Mitiglinide誘導体 |
Outline of Final Research Achievements |
To develop such a method to measure β-cell mass (BCM), we took advantage of the previously reported non-invasive detection of glucagon-like peptide-1 receptor using radiolabeled ligand. GLP-1R is highly expressed in rodent and human pancreatic β cells. We selected Exendin-4 (Ex4), a stable agonist of GLP-1R, as the mother compound and bound 111In to it using diethylenetriamine pentaacetic acid (DTPA) as a chelating moiety. 111In-DTPA-Ex4 produced as a probe for single photon emission computed tomography (SPECT) showed a high affinity for GLP-1R in vitro. We have performed SPECT imaging in normal and β-cell loss mice and determined a correlation between the uptake of 111In-DTPA-Ex4 in the pancreas and BCM to investigate the potential for the in vivo quantification of BCM by SPECT with 111In-DTPA-Ex4. This imaging technique would be promising tools for islet transplantation research.
|
Report
(3 results)
Research Products
(11 results)
-
-
[Journal Article] Synthesis and evaluation of 18F-labeled mitiglinide derivatives as positron emission tomography tracers for β-cell imaging2014
Author(s)
Hiroyuki Kimura, Hirokazu Matsuda, Hiroyuki Fujimoto, Kenji Arimitsu, Kentaro Toyoda, Eri Mukai, Hiroshi Nakamura, Yu Ogawa, Mikako Takagi, Masahiro Ono, Nobuya Inagaki, Hideo Saji
-
Journal Title
Bioorganic & Medicinal Chemistry
Volume: 未定
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-