Analysis of irradiation effects on post-transcriptional control of gene expression by a newly developed method

• Project/Area Number: 25670542
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Kurume University
• Principal Investigator: OKU Eijiro 久留米大学, 医学部, 講師 (10569429)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 遺伝子発現調節 / 放射線生物学 / 放射線

Outline of Final Research Achievements

Recently, post-transcriptional control of gene expression, mediated by such as microRNA and/or RNA-binding protein, has been considered to play an important role in gene regulation. In this study, we developed a novel method to discover a set of genes of post-transcriptionally regulation using retrovirus vector system. In order to find out genes with post-transcriptionally regulation affected by radiation, we applied this method to pleuripotent stem cell as well as hematopoietic stem cell with or without irradiation and could detect sets of genes of interest. We will perform further investigation to find out genes for therapeutic targets and to clarify the underlying mechanism of translational control.

Research Products

• [Journal Article] Donor-derived 47, XXY in an unrelated cord blood transplant recipient. (2014)
  • Author(s): Kawaguchi K, Nakamura T, Nohara M, Koteda S, Nomura K, Morishige S, Oku E, Imamura R, Mouri F, Seki R, Osaki K, Hashiguchi M, Yoshimoto K, Nagafuji K, Okamura T.
  • Journal Title: Springerplus. Volume: 6 Issue: 1 Pages: 72-72
  • DOI: 10.1186/2193-1801-3-72
  • Peer Reviewed
• [Journal Article] Fatal zygomycosis caused by Mucor indicus after haplo-identical stem cell transplantation (2013)
  • Author(s): Koteda S, Nomura K, Hashiguchi M, Kawaguchi K, Oku E, Osaki K, Nakamura T, Mouri F, Imamura R, Seki R, Nagafuji K, Makimura K, Okamura T.
  • Journal Title: Rinsho Ketsueki. Volume: 54(3) Pages: 311-315
  • NAID: 10031165622
  • Peer Reviewed
• [Journal Article] Successful treatment of small cell variant anaplastic large cell lymphoma with allogeneic peripheral blood stem cell transplantation, and review of the literature (2013)
  • Author(s): Imamura R, Mouri F, Oku E, Okamura T
  • Journal Title: Int J Hematol Volume: 97 Issue: 1 Pages: 139-143
  • DOI: 10.1007/s12185-012-1242-3
  • NAID: 10031144646
  • Peer Reviewed
• [Journal Article] A case of 8p11 myeloproliferative syndrome with BCR-FGFR1 gene fusion presenting with trilineage acute leukemia/lymphoma, successfully treated by cord blood transplantation (2013)
  • Author(s): Morishige S, Oku E, Mizuno S, Okamura T
  • Journal Title: Acta Haematol Volume: 129 Issue: 2 Pages: 83-89
  • DOI: 10.1159/000341289
  • Peer Reviewed