| Project/Area Number |
25670547
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKETOMI Akinobu 北海道大学, 医学(系)研究科(研究院), 教授 (70363364)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝移植 / 機械灌流 / グラフト治療 / 機械灌流保存 / 臓器保存 |
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| Outline of Final Research Achievements |
We conducted this study to develop an extracorporeal siRNA transfection method during machine perfusion. Because siRNA could not be transfected under hypothermic condition and perfusion fluid without blood components was preferable as a carrier of siRNA, we achieved sub-normothermic oxygenated machine perfusion (20℃, 180min) in mouse liver transplantation model. We performed extracorporeal siRNA transfection on this mouse model, however, siRNA could not be introduced into liver grafts under such perfusion conditions. According to the results of donor siRNA transfection model, it was suggested that siRNA up-take activities of the liver grafts were not maintained at sufficient levels at 20℃.
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