| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We investigated whether preoperative transplant of recipient-derived bone marrow cells (BMCs) to the donor attenuated the rejection after liver transplantation in the rat acute rejection model. Rats in the BMC group experienced prolonged survival. Serological and histopathological analyses revealed that the rejection of the liver graft was attenuated in the BMC group. X-gal staining and immunohistostaining of the liver graft revealed that transplanted BMCs were engrafted in the sinusoidal space and differentiated into Kupffer cells. Preoperative transplantation of recipient BMCs to LT donors replaced donor Kupffer cells and attenuated post-LT rejection, indicating that this strategy may increase the success of LT. We are planning further investigations with xenotransplantation models. We established the hamster/rat liver xenotransplantation model, which is similar to pig/human xenotransplantation.
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