Identification of novel inducing factors pancreatic beta cells by iPS modified technology

• Project/Area Number: 25670556
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: ISHII Hideshi 大阪大学, 医学(系)研究科(研究院), 特任教授(常勤) (10280736)
• Co-Investigator(Kenkyū-buntansha): KONNO Masamitsu 大阪大学, 大学院医学系研究科, 寄附講座助教 (80618207)
• Co-Investigator(Renkei-kenkyūsha): MORI Masaki 大阪大学, 大学院医学系研究科, 教授 (70190999) ; MIMORI Koshi 九州大学, 大学病院, 教授 (50322748) ; NAGANO Hiroaki 山口大学, 大学院医学系研究科, 教授 (10294050) ; EGUCHI Hidetoshi 大阪大学, 大学院医学系研究科, 准教授 (90542118)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 再生医療 / 再生医学 / 細胞・組織 / 発生・分化 / トランスレーショナルリサーチ

Outline of Final Research Achievements

To identity critical factors for induction of pancreatic beta cells, we developed novel technology of induced pluripotent stem (iPS) interfering method. The study of gene expression allowed the identification of several factors, which play roles in induction and maintenance of pancreatic beta cells. The present study would open an avenue to new efficient approach for human diabetes.

Research Products

• [Journal Article] Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. (2014)
  • Author(s): Yoshioka Y, Kosaka N, Konishi Y, Ohta H, Okamoto H, Sonoda H, Nonaka R, Yamamoto H, Ishii H, Mori M, Furuta K, Nakajima T, Hayashi H, Sugisaki H, Higashimoto H, Kato T, Takeshita F, Ochiya T.
  • Journal Title: Nat. Commun. Volume: 5 Pages: 3591-3591
  • DOI: 10.1038/ncomms4591
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] LZTS1 downregulation confers paclitaxel resistance and is associated with worse prognosis in breast cancer. (2014)
  • Author(s): Lovat, F., Ishii, H., Schiappacassi, M., Fassan, M., Barbareschi, M., Galligioni, E., Gasparini, P., Baldassarre, G., Croce, CM., Vecchione, A.
  • Journal Title: Oncotarget Volume: 5 Pages: 970-977
  • Peer Reviewed
• [Journal Article] CD10 as a novel marker of therapeutic resistance and cancer stem cells in head and neck squamous cell carcinoma. (2014)
  • Author(s): Fukusumi T, Ishii H, Konno M, Yasui T, Nakahara S, Takenaka Y, Yamamoto Y, Nishikawa S, Kano Y, Ogawa H, Hasegawa S, Hamabe A, Haraguchi N, Doki Y, Mori M, Inohara H.
  • Journal Title: Br. J. Cancer Volume: 111(3) Pages: 506-514
  • DOI: 10.1038/bjc.2014.289
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MicroRNA-1246 expression associated with CCNG2-mediated chemoresistance and stemness in pancreatic cancer. (2014)
  • Author(s): Hasegawa S, Eguchi H, Nagano H, Konno M, Tomimaru Y, Wada H, Hama N, Kawamoto K, Kobayashi S, Nishida N, Koseki J, Nishimura T, Gotoh N, Ohno S, Yabuta N, Nojima H, Mori M, Doki Y, Ishii H.
  • Journal Title: B.r J. Cancer Volume: 111 Pages: 1572-1580
  • DOI: 10.1038/bjc.2014.454
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Combined evaluation of hexokinase 2 and phosphorylated pyruvate dehydrogenase-E1α in invasive front lesions of colorectal tumors predicts cancer metabolism and patient prognosis. (2014)
  • Author(s): Hamabe, A. Yamamoto, H. Konno, M. Uemura, M. Nishimura, J. Hata, T. Takemasa, I. Mizushima, T. Nishida, N. Kawamoto, K. Koseki, J. Doki, Y. Mori, M. Ishii, H.
  • Journal Title: Cancer Sci. Volume: 105 Pages: 1100-1108
  • DOI: 10.1111/cas.12487
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of pyruvate kinase M2 in transcriptional regulation leading to epithelial-mesenchymal transition. (2014)
  • Author(s): Hamabe, A. Konno, M. Tanuma, N. Shima, H. Tsunekuni, K. Kawamoto, K. Nishida, N. Koseki, J. Mimori, K. Gotoh, N. Yamamoto, H. Doki, Y. Mori, M. Ishii, H.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Pages: 15526-15531
  • DOI: 10.1073/pnas.1407717111
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cancer Stem Cells of the Digestive System. (2014)
  • Author(s): Colvin, S. H., Nishida, N., Koseki, J., Konno, M., Kawamoto, K., Tsunekuni, K., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Jpn. J. Clin. Oncol. Volume: 44 Pages: 1141-1149
  • DOI: 10.1093/jjco/hyu146
  • Peer Reviewed
• [Journal Article] SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers. (2013)
  • Author(s): Suzuki, Y., Haraguchi, N., Takahashi, H., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Mizushima, T., Ishii, H., Doki, Y., Mori, M., Yamamoto, H.
  • Journal Title: Int. J. Oncol Volume: 42 Pages: 161-167
  • DOI: 10.3892/ijo.2012.1713
  • Peer Reviewed
• [Journal Article] Depletion of JARID1B induces cellular senescence in human colorectal cancer. (2013)
  • Author(s): Ohta, K., Haraguchi, N., Kano, Y., Kagawa, Y., Konno, M., Nishikawa, S., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Nishimura, T., Noguchi, Y., Ozaki, M., Kudo, T., Sakai, D., Satoh, T., Fukami, M., Ishii, M., Yamamoto, H., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Int. J. Oncol. Volume: 42 Pages: 1212-1218
  • DOI: 10.3892/ijo.2013.1799
  • Peer Reviewed
• [Journal Article] Aldehyde dehydrogenasehigh gastric cancer stem cells are resistant to chemotherapy. (2013)
  • Author(s): Nishikawa, S., Konno, M., Hamabe, A., Hasegawa, S., Kano, Y., Ohta, K., Fukusumi, T., Sakai, D., Kudo, T., Haraguchi, N., Satoh, T., Takiguchi, S., Mori, M., Doki,Y., Ishii, H.
  • Journal Title: Int J Oncol. Volume: 42 Pages: 1437-1442
  • DOI: 10.3892/ijo.2013.1837
  • Peer Reviewed
• [Journal Article] Adipose-derived mesenchymal stem cells and regenerative medicine (2013)
  • Author(s): Konno, M., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Nishikawa, S., Ohta, K., Kano, Y., Ozaki, M., Noguchi, Y., Sakai, D., Kudoh, T., Kawamoto, K., Eguchi, H., Satoh, T., Tanemura, M., Nagano, H., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Dev Growth Differ Volume: 55 Pages: 309-18
  • DOI: 10.1111/dgd.12049
  • NAID: 40019735006
  • Peer Reviewed
• [Presentation] Innovative drug discovery for intractable cancer stem cells (2014)
  • Author(s): 石井秀始
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪）
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] 難治性消化器癌に対する革新的治療法 (2014)
  • Author(s): 石井秀始、土岐祐一郎、森正樹
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 創薬標的としての癌幹細胞エピゲノム代謝 (2014)
  • Author(s): 石井秀始
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Innvovative Medicine for Gastrointestinal Cancer Stem Cells (2014)
  • Author(s): 石井秀始
  • Organizer: 第52回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川）
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] 難治性消化器癌に対する革新的医療技術の創出 (2013)
  • Author(s): 石井秀始、土岐祐一郎、森正樹
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 難治性消化器癌を克服する新しい創薬展開 (2013)
  • Author(s): 石井 秀始
  • Organizer: 第23回日本サイトメトリー学会学術集会
  • Place of Presentation: 日本医科大学橘桜会館（東京都）