Functional analysis of novel genes based on molecular evolution

• Project/Area Number: 25670574
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Nagoya University
• Principal Investigator: Yokoyama Yukihiro 名古屋大学, 医学部附属病院, 講師 (80378091)
• Co-Investigator(Kenkyū-buntansha): NAGINO MASATO 名古屋大学, 医学系研究科, 教授 (20237564) ; KOKURYO TOSHIO 名古屋大学, 医学系研究科, 特任講師 (60378023)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 分子進化

Outline of Final Research Achievements

Various amino acids, such as tyrosine, leucine, methionine, tryptophan, asparagine, aspartic acid, alanine, arginine, isoleucine, glycine, glutamine, cysteine, serine, histidine, proline, lysine, valine, threonine and phenylalanine, induce altered expression of endothelin 1 in RAW cells after LPS administration. Cysteine (40 mM) suppressed endothelin 1 expression and the induction of iNOS in RAW cells. Administration of several amino acids induced significant alterations in intracellular calcium concentrations. These results suggest that amino acids are related to calcium concentrations both inside and outside the cell.