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Fluorescent virus-guided capturing of epithelial-mesenchymal transition (EMT)-induced circulating tumor cells (CTCs) for genetic analysis

Research Project

Project/Area Number 25670580
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionOkayama University

Principal Investigator

FUJIWARA Toshiyoshi  岡山大学, 医歯(薬)学総合研究科, 教授 (00304303)

Co-Investigator(Kenkyū-buntansha) KAGAWA Shunsuke  岡山大学, 大学病院, 准教授 (00362971)
SHIRAKAWA Yasuhiro  岡山大学, 大学院医歯薬学総合研究科, 准教授 (60379774)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords循環癌細胞 / 上皮間葉転換 / テロメラーゼ / アデノウイルス / TGF-beta / GFP / 遺伝子解析 / PCR
Outline of Final Research Achievements

Molecular-based companion diagnostic tests are being used with increasing frequency to predict their clinical response to various drugs. However, invasive procedures are typically required to obtain tissues for this analysis. Here we established a new approach to capture live circulating tumor cells (CTCs) among millions of peripheral blood leukocytes using a GFP-expressing attenuated adenovirus, in which the telomerase promoter regulates viral replication (TelomeScan). Because current CTC detection strategies mainly depend on epithelial cell surface markers, the presence of heterogeneous populations of CTCs with epithelial and/or mesenchymal characteristics may pose obstacles to the detection of CTCs. Our biological capturing system can image both epithelial and mesenchymal tumor cells with telomerase activities as GFP-positive cells. This fluorescent virus-guided viable CTC capturing method provides a non-invasive alternative to tissue biopsy.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (2 results)

  • [Journal Article] Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer.2015

    • Author(s)
      Kikuchi S, Kishimoto H, Tazawa H, Hashimoto Y, Kuroda S, Nishizaki M, Nagasaka T, Shirakawa Y, Kagawa S, Urata Y, Hoffman RM, Fujiwara T.
    • Journal Title

      Mol Ther.

      Volume: 23(3) Issue: 4 Pages: 501-509

    • DOI

      10.1136/gutjnl-2014-306957

    • NAID

      120005537574

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer.2015

    • Author(s)
      Kikuchi S, et al.
    • Journal Title

      Molecular Therapy

      Volume: 23 Issue: 3 Pages: 501-509

    • DOI

      10.1038/mt.2014.244

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Establishment of a non-invasive semi-quantitative bioluminescent imaging method for monitoring of an orthotopic esophageal cancer mouse model.2014

    • Author(s)
      Kuroda S, et al.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 12 Pages: e114562-e114562

    • DOI

      10.1371/journal.pone.0114562

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Selective methioninase-induced trap of cancer cells in S/G2 phase visualized by FUCCI imaging confers chemosensitivity.2014

    • Author(s)
      Yano S, et al.
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 8729-8736

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial-temporal FUCCI imaging of each cell in a tumor demonstrates locational dependence of cell cycle dynamics and chemoresponsiveness.2014

    • Author(s)
      Yano S, et al.
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 13 Pages: 2110-2119

    • DOI

      10.4161/cc.29156

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual programmed cell death pathways induced by p53 transactivation overcome resistance to oncolytic adenovirus in human osteosarcoma cells.2013

    • Author(s)
      Hasei, J.
    • Journal Title

      Molecular Cancer Therapeutics

      Volume: 12 Issue: 3 Pages: 314-325

    • DOI

      10.1158/1535-7163.mct-12-0869

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel synergistic effect of iron depletion on antiangiogenic cancer therapy.2013

    • Author(s)
      Ohara T, Noma K, Urano S, Watanabe S, Nishitani S, Tomono Y, Kimura F, Kagawa S, Shirakawa Y, Fujiwara T
    • Journal Title

      International Journal of Cancer

      Volume: 132 Issue: 11 Pages: 2705-2713

    • DOI

      10.1002/ijc.27943

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multicenter phase II study of S-1 and docetaxel combination chemotherapy for advanced or recurrent gastric cancer patients with peritoneal dissemination.2013

    • Author(s)
      Shigeyasu, K.
    • Journal Title

      Cancer Chemotherapy and Pharmacology

      Volume: 71 Issue: 4 Pages: 937-943

    • DOI

      10.1007/s00280-013-2086-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A genetically engineered oncolytic adenovirus decoys and lethally traps quiescent cancer stem-like cells into S/G2/M-phases.2013

    • Author(s)
      Yano S, Tazawa H, Hashimoto Y, Shirakawa Y, Kuroda S, Nishizaki M, Kishimoto H, Uno F, Nagasaka T, Urata Y, Kagawa S, Hoffman RM, Fujiwara T
    • Journal Title

      Clin Cancer Res

      Volume: 19 Issue: 23 Pages: 6495-6505

    • DOI

      10.1158/1078-0432.ccr-13-0742

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A simple detection system for adenovirus receptor expression using a telomerase-specific replication-competent adenovirus2012

    • Author(s)
      Sasaki, T.
    • Journal Title

      Gene Therapy

      Volume: (Epub ahead of print) Issue: 1 Pages: 112-118

    • DOI

      10.1038/gt.2011.213

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 胃癌における蛍光発現ウイルスによる腹腔洗浄液診断:生物学的悪性度指標としての可能性.2013

    • Author(s)
      香川俊輔
    • Organizer
      第113回日本外科学会定期学術集会(シンポジウム)
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] テロメラーゼ活性を指標とする血中遊離癌細胞の高感度検出法の開発と遺伝子解析技術への応用.2013

    • Author(s)
      重安邦俊
    • Organizer
      第113回日本外科学会定期学術集会(ワークショップ)
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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