Generation of artificial lung cancer stem cells and its regulation by microenvironment in order to develop the effective therapeutic strategy for lung cancer.

Research Project

Project/Area Number	25670611
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Respiratory surgery
Research Institution	Miyagi Prefectural Hospital Organization Miyagi Cancer Center
Principal Investigator	ABE Jiro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), その他部局等, 特任研究員 (10573686)
Co-Investigator(Kenkyū-buntansha)	SATOH Kennichi 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん幹細胞研究部, 部長 (10282055)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	肺癌 / 人工がん幹細胞 / HOTAIR / 癌幹細胞
Outline of Final Research Achievements	In order to develop the new therapeutic strategy for non-small cell lung cancer (NSCLC)　stem cell, we tried to establish artificial cancer stem cell line. As cancer stem cell related molecule, we focused on large non-coding RNA HOTAIR. Lung cancer cells (A549) showed increased cellular migration in HOTAIR dependent munner. In addition, the expression of HOTAIR was found especially in aggressive NSCLC and was significantly correlated with shorter disease free survival, suggesting that HOTAIR might be involved in the property of cancer stem cell of NSCLC.