Are proton-sensing receptors working in ischemia~analysis with receptor-deficient mice
Project/Area Number |
25670617
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurosurgery
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Research Institution | Gunma University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | プロトン / 虚血再灌流 / 脳梗塞 / ミクログリア / 神経細胞死 / GPCR / TDAG8 / OGR1 / 中枢神経 |
Outline of Final Research Achievements |
It is well known that acidification takes place in the ischemia region. However, how acidic pH regulates neuronal cell functions remains uncharacterized yet. In the present study, we characterized the roles of OGR1 family GPCRs, which have been recently identified as extracellular proton-sensing receptors, in ischemia reperfusion model in vivo and in mouse microglia and cultured N1E115 cells as neuronal cell model in vitro. We found that TDAG8 is functioning as protective for brain damage after the ischemia reperfusion. TDAG8 in microglia might be involved in the protective effects. We also found that acidic pH activates nNOS/cGMP signaling through OGR1 in N1E115 cells. Thus, OGR1 family GPCRs are involved in the regulation of brain functions.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Extracellular acidification activates ovarian cancer G-protein-coupled receptor 1 and GPR4 homologs of zebra fish.2015
Author(s)
Y. Mochimaru, M. Azuma, N. Oshima, Y. Ichijo, K. Satou, K. Matsuda, Y. Asaoka, H. Nishina, T. Nakakura, C. Mogi, K. Sato, F. Okajima, H. Tomura
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 457
Issue: 4
Pages: 493-499
DOI
Related Report
Peer Reviewed
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[Journal Article] Acidic pH increases cGMP accumulation through the OGR1/phospholipase C/Ca2+/neuronal NOS pathway in N1E-115 neuronal cells.2014
Author(s)
M. Kotake, K. Sato, C. Mogi, M. Tobo, H. Aoki, T. Ishizuka, N. Sunaga, H. Imai, K. Kaira, T. Hisada, M. Yamada, F. Okajima
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Journal Title
Cell. Signal.
Volume: 26
Issue: 11
Pages: 2326-2332
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Proton-sensing ovarian cancer G protein-coupled receptor 1 on dendritic cells is required for airway responses in a murine asthma model2013
Author(s)
Aoki H, Mogi C, Hisada T, Kamide Y, Ichimonji I, Tomura H, Tobo M, Sato K, Tsurumaki H, Dobashi K, Mori T, Harada H, Yamada M, Mori M, Ishizuka T, Okajima F
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Journal Title
PLoS ONE
Volume: 8(11)
Issue: 11
Pages: e79985-e79985
DOI
Related Report
Peer Reviewed
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[Presentation] Critical role of proton-sensing OGR1 in dendritic cells in development of asthma2014
Author(s)
Haruka Aoki, Chihiro Mogi, Takeshi Hisada, Takashi Nakakura, Yosuke Kamide, Isao Ichimonji, Hideaki Tomura, Masayuki Tobo, Koichi Sato, Hiroaki Tsurumaki, Kunio Dobashi, Tetsuya Mori, Akihiro Harada, Masatomo Mori, Tamotsu Ishizuka, Fumikazu Okajima
Organizer
International cymposium on "Homeostasis through development, life, and diseases."
Place of Presentation
Tojo Hall, Gunma University
Year and Date
2014-11-07
Related Report
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