Light-induced regulation of the function of osteocyte and bone remodelling

• Project/Area Number: 25670638
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Osaka Dental University (2014); Tokyo Medical and Dental University (2013)
• Principal Investigator: NOTOMI Takuya 大阪歯科大学, 歯学部, 講師 (70542249)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨代謝 / 力学的刺激

Outline of Final Research Achievements

Age-related bone diseases such as osteoporosis are serious problem in an aged society and their treatment is necessary for maintaining QOL. To prevent bone diseases, we have done the study of role of membrane potential and ion channels on bone cells. To challenge the regulation of osteocyte function in vitro culture, we overexpressed light-sensitive ion channel and pump. The light-sensitive ion channels and pump were transfected into osteocyte and then we investigated the role of membrane potential on those cells. In electrophysiological experiments, the membrane potential was controlled by light stimulus. The light stimulus regulated some portion of osteocyte function. In summary, our results indicate that membrane potential regulate some of osteocyte functions. Our system could regulate the membrane potential and function in osteocyte.

Research Products

• [Journal Article] TGF-Beta Suppresses Ift88 Expression in Chondrocytic ATDC5 cells (2015)
  • Author(s): Makiri Kawasaki, Yoichi Ezura, Tadayoshi Hayata, Takuya Notomi, Yayoi Izu, Masaki Noda
  • Journal Title: J Cell Physiol Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Zinc-induced effects on osteoclastogenesis involves activation of hyperpolarization-activated cyclic nucleotide modulated channels via changes in membrane potential (2015)
  • Author(s): Takuya Notomi (Corresponding author), Miyuki Kuno, Akiko Hiyama, Kiyoshi Ohura, Masaki Noda, Timothy M. Skerry
  • Journal Title: J Bone Miner Res Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] β2 Adrenergic receptor activation suppresses BMP-induced alkaline phosphatase expression in osteoblast-like MC3T3E1 cells (2015)
  • Author(s): Takayuki Yamada, Yoichi Ezura, Tadayoshi Hayata, Shuichi Moriya, Jumpei Shirakawa, Takuya Notomi, Smriti Aryal, Makiri Kawasaki, Yayoi Izu, Kiyoshi Harada, Masaki Noda
  • Journal Title: J Cell Biochem Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] PTH regulates β2-adrenergic receptor expression in osteoblast-like MC3T3-E1 cells (2015)
  • Author(s): Shuichi Moriya, Tadayoshi Hayata, Takuya Notomi, Smriti Aryal, Testuya Nakamoto, Yayoi Izu, Makiri Kawasaki, Takayuki Yamada, Jumpei Shirakawa, Kazuo Kaneko, Yoichi Ezura, Masaki Noda
  • Journal Title: J Cell Biochem Volume: 116 Pages: 142-148
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Insulinogenic sucrose + amino acid mixture ingestion immediately after resistance exercise has an anabolic effect on bone compared with non-insulinogenic fructose + amino acid mixture in growing rats (2014)
  • Author(s): Takuya Notomi (Corresponding author), Ikuaki Karasaki, Yuichi Okazaki, Nobukazu Okimoto, Yushi Kato, Kiyoshi Ohura, Masaki Noda, Toshitaka Nakamura, Masashige Suzuki
  • Journal Title: Bone Volume: 65 Pages: 42-48
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Migration linked to FUCCI-indicated cell cycle is controlled by PTH and mechanical stress (2014)
  • Author(s): Jumpei Shirakawa, Yoichi Ezura, Shuichi Moriya, Makiri Kawasaki, Takayuki Yamada, Takuya Notomi, Tetsuya Nakamoto, Tadayoshi Hayata, Atsushi Miyawaki, Ken Omura, Masaki Noda
  • Journal Title: J Cell Physiol Volume: -
  • Peer Reviewed
• [Journal Article] Stability of mRNA influences osteoporotic bone mass via Cnot3 (2014)
  • Author(s): Chiho Watanabe, Masahiro Morita, Yoichi Ezura, Tetsuya Nakamoto, Tadayoshi Hayata, Chisato Kikuguchi, Li Xue, Yasuhiro Kobayashi, Naoyuki Takahashi, Takuya Notomi, Keiji Moriyama, Tadashi Yamamoto, Masaki Noda
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Pages: 2692-7
  • Peer Reviewed
• [Journal Article] Osteoblastic differentiation enhances expression of TRPV4 that is required for calcium oscillation induced by mechanical force (2013)
  • Author(s): Takafumi Suzuki, Takuya Notomi (Corresponding author), Daizuke Miyajima, Fumitaka Mizoguchi, Tadayoshi Hayata, Tetsuya Nakamoto, Ryo Hanyu, Paksinee Kamolratanakul, Atsuko Mizuno, Makoto Suzuki, Yoichi Ezura, Yuichi Izumi, Masaki Noda
  • Journal Title: Bone Volume: 54 Pages: 172-8
  • Peer Reviewed
• [Journal Article] Nck1 deficiency accelerates unloading-induced bone loss (2013)
  • Author(s): Aryal Smiriti, Kentaro Miyai, Yoichi Ezura, Tadayoshi Hayata, Takuya Notomi, Tetsuya Nakamoto, Tony Pawson, Masaki Noda
  • Journal Title: J. Cell. Physiol. Volume: 228 Pages: 1397-1403
  • Peer Reviewed
• [Journal Article] 骨研究フロンティア-基礎から臨床まで- 骨形成・骨吸収における関連因子の働き (2013)
  • Author(s): 野田政樹、江面陽一、早田匡芳、守屋秀一、白川純平、Smiriti Arayal、納富拓也、渡辺千穂、長尾雅史、羽生亮
  • Journal Title: 日本臨牀 Volume: 71 Pages: 161-166
• [Presentation] レジスタンス運動直後のインスリン高刺激性糖摂取は、インスリン低刺激性糖摂取に比べて骨量・骨強度を増大させる (2014)
  • Author(s): 納富拓也、大浦清、野田政樹
  • Organizer: 第56回 歯科基礎医学会
  • Place of Presentation: 福岡県、福岡市、福岡国際会議場
  • Year and Date: 2014-09-26
• [Presentation] スフィンゴシン1リン酸はin vitroの破骨細胞形成系において分化促進する (2014)
  • Author(s): 天野均、納富拓也、大浦清
  • Organizer: 第56回 歯科基礎医学会
  • Place of Presentation: 福岡県、福岡市、福岡国際会議場
  • Year and Date: 2014-09-26
• [Presentation] Insulinogenic sucrose+amino acids mixture ingestion immediately after resistance exercise has an anabolic effect on bone compared with non-insulinogenic fructose+amino acids mixture in growing rats (2014)
  • Author(s): Takuya Notomi, Ikuaki Karasaki, Yuichi Okazaki, Nobukazu Okimoto, Yushi Kato, Kiyoshi Ohura, Masaki Noda, Toshitaka Nakamura, Masashige Suzuki
  • Organizer: ASBMR 2014
  • Place of Presentation: テキサス州、米国
  • Year and Date: 2014-09-13
• [Presentation] Preferentially expressed genes in synovium derived stromal cells include atypical genes not expressed highly in mouse synovium but in embryonic cartilages (2014)
  • Author(s): Yoichi Ezura, Tadayoshi Hayata, Takuya Notomi, Ichiro Sekiya, Masaki Noda
  • Organizer: ASBMR 2014
  • Place of Presentation: テキサス州、米国
  • Year and Date: 2014-09-13
• [Presentation] レジスタンス運動直後のインスリン高刺激性糖（砂糖）＋アミノ酸溶液摂取は、インスリン低刺激性糖（果糖）＋アミノ酸溶液摂取に比べて骨量・骨強度を増大させる (2014)
  • Author(s): 納富拓也、唐崎郁晃、岡崎雄一、沖本信和、加藤雄士、大浦清、野田政樹、中村利孝、鈴木正成
  • Organizer: 第32回 日本骨代謝学会
  • Place of Presentation: 大阪府、大阪市、大阪国際会議場
  • Year and Date: 2014-07-25
• [Presentation] 培養軟骨細胞ATDC5において、TGF-beta1は一次繊毛構成遺伝子Ift88の発現を抑制し、一次繊毛を短縮させる (2014)
  • Author(s): 川崎真希理、早田匡芳、中元哲也、納富拓也、江面陽一、野田政樹
  • Organizer: 第32回 日本骨代謝学会
  • Place of Presentation: 大阪府、大阪市、大阪国際会議場
  • Year and Date: 2014-07-25
• [Presentation] Depolarizing Membrane Potential by PTH and VD3 Regulates RANKL-intracellular Transportation; A Novel Mechanism of PTH- and VD3-induced Osteoclastogenesis
  • Author(s): Takuya Notomi, Miyuki Kuno, Yoichi Ezura, Masaki Noda
  • Organizer: ASBMR 2013
  • Place of Presentation: メリーランド州、米国
• [Presentation] Genes significantly highly expressed in synovium derived stromal cells than in bone marrow derived cells are conserved both in mouse and human, and may contribute to higher potential for chondrogenic differentiation
  • Author(s): Yoichi Ezura, Tadayoshi Hayata, Takuya Notomi, Ichiro Sekiya, Masaki Noda
  • Organizer: ASBMR 2013
  • Place of Presentation: メリーランド州、米国
• [Presentation] PTH Additively Enhances The Mechanical Stress-induced Proliferation of Calvarial Osteoblasts
  • Author(s): Jumpei Shirakawa, Yoichi Ezura, Makiri Kawasaki, Takayuki Yamada, Shuichi Moriya, Takuya Notomi, Tadayoshi Hayata, Ken Omura, Masaki Noda
  • Organizer: ASBMR 2013
  • Place of Presentation: メリーランド州、米国
• [Presentation] TGF-β1 inhibits maturation of chondrogenic cell line ATDC5 by impeding canonical hedgehog signaling through direct down-regulation of ciliary component gene Ift88
  • Author(s): Makiri Kawasaki, Tetsuya Nakamoto, Takuya Notomi, Tadayoshi Hayata, Yoichi Ezura, Masaki Noda
  • Organizer: ASBMR 2013
  • Place of Presentation: メリーランド州、米国
• [Presentation] Tob1, a BMP repressor, is activated by parathyroid hormone in osteoblasts in vitro and in vivo and reciprocally regulates PTH signaling
  • Author(s): Shuichi Moriya, Tadayoshi Hayata, Jumpei Shirakawa, Makiri Kawasaki, Takuya Notomi, Yoichi Ezura, Kazuo Kaneko, Masaki Noda
  • Organizer: ASBMR 2013
  • Place of Presentation: メリーランド州、米国
• [Presentation] β2Adrenergic Receptor agonist suppresses BMP-induced osteoblastic differentiation in MC3T3E-1 cells while epinephrine modulates it differently
  • Author(s): Takayuki Yamada, Tadayoshi Hayata, Takuya Notomi, Yoichi Ezura, Kiyoshi Harada, Masaki Noda
  • Organizer: ASBMR 2013
  • Place of Presentation: メリーランド州、米国
• [Presentation] 破骨細胞分化段階における細胞内Ca2+動態とイオンチャネルの役割
  • Author(s): 納富拓也、江面陽一、野田政樹
  • Organizer: 日本薬理学会年会 2014
  • Place of Presentation: 仙台国際センター
  • Invited
• [Presentation] 滑膜・半月板および靭帯由来間葉系幹細胞における選択的発現遺伝子の同定
  • Author(s): 江面陽一、早田匡芳、中元哲也、納富拓也、関谷一郎、宗田大、野田政樹
  • Organizer: 日本骨代謝学会 2014
  • Place of Presentation: 神戸国際会議場