| Project/Area Number |
25670648
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Keiichiro 岡山大学, 大学院医歯薬学総合研究科, 准教授 (80284058)
KAKUTA Hiroki 岡山大学, 大学院医歯薬学総合研究科, 准教授 (80362961)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | プロテオグリカン / 変形性関節症 / X線 / 動物モデル / 創薬 / 軟骨 / 蛍光 / X線造影剤 |
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| Outline of Final Research Achievements |
In the process of cartilage degeneration seen in osteoarthritis, loss of proteoglycan from articular cartilage has been widely accepted as a critical early event. We investigated bio-molecular imaging of articular cartilage using our molecular probes targeting articular GAGs.
Chondroitin sulfate, a major component of articular cartilage, has negative charge caused by sulfates. We designed lysine oligomers (monomer - pentamer) which were connected with the a-carboxyl and e-amino group. These lysine oligomers possess e-amino groups as cationic moieties. Then, we created a novel articular cartilage imaging X-ray probe Ke4-triiodobenzene (Ke4-TIB) and demonstrated an ex vivo imaging of articular cartilage in rat osteoarthritic models. The imaging could quantitate loss of proteoglycan from osteoarthritic cartilage. Ke4-TIB may have a potential of in vivo X-ray imaging in animal arthritic models in future, which contribute to drug discovery research for osteoarthritis.
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