| Project/Area Number |
25670649
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAKAWA Yasuaki 岡山大学, 大学病院, 医員 (90636953)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | oncolytic adenovirus / circulating tumor cell / sarcoma / biomarker / CTC / テロメスキャン / 骨軟部肉腫 |
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| Outline of Final Research Achievements |
We investigated the usefulness of telomerase specific oncolytic adenovirus containing the green fluorescent protein (GFP), which are OBP-401 or OBP-1101, to detect circulating tumor cells of sarcoma as a new biomarker. 55 sarcoma patients were examined, and CTCs were detected in 11 of 22 patients (50%) with OBP-401 and 2 of 33 patients (6%) with OBP-1101. We compare OBP-401 and OBP-1101 in CTC experimental model with 5 types of sarcoma cell lines, but two virus showed same detection rate. We identified whether CTCs are really tumor cells or not by proliferate the tumor specific genome with PCR, but it was impossible due to technical difficulty.
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