Research Project
Grant-in-Aid for Challenging Exploratory Research
Endotoxin/lipopolysaccharide (LPS) preconditioning (PC) has been shown to attenuate damage induced by stroke and brain trauma in rodent models. The present study preconditioned mice with LPS before inducing contusion SCI to investigate the effect of PC. We found that PC promotes the polarization of M1/M2 macrophages/microglia toward an M2 phenotype in the injured spinal cord on quantitative real-time polymerase chain reaction. Flow cytometric analyses reveal that PC facilitates M2 activation in resident microglia. Augmented M2 activation was accompanied by tissue reorganization and functional recovery. Altogether, our findings demonstrate that LPS preconditioning has a therapeutic effect on SCI through the modulation of M1/M2 polarization of resident microglia. The present study suggests that controlling M1/M2 polarization through endotoxin signal transduction could become a promising therapeutic strategy for various central nervous system diseases.
All 2014 Other
All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)
J Neurosci Res.
Volume: 92(12) Issue: 12 Pages: 1647-58
10.1002/jnr.23448
