In vivo imaging for joint, blood, and cerebrospinal fluid

• Project/Area Number: 25670662
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Spinal Injuries Center
• Principal Investigator: SHIBA KEIICHIRO 独立行政法人労働者健康福祉機構総合せき損センター(研究部), その他部局等, 研究員 (90574137)
• Co-Investigator(Renkei-kenkyūsha): OKADA SEIJI 九州大学, 医学研究院, 准教授 (30448435)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: バイオイメージング

Outline of Final Research Achievements

The fluid biology including blood, cerebrospinal and joint fluid, is closely related with the pathophysiology of most disease in the field of orthopedic surgery. However, the evaluating method for it is relatively difficult due to a lack of basic researches using animal model. We attempted to establish in vivo imaging of fluid biology by using firefly luciferase and artificial albumin. We demonstrated that in vivo imaging of accumulated inflammatory cells can predict the prognosis in the hyperacute phase of spinal cord injury.

Research Products

• [Journal Article] Rapid progressive clinical deterioration of cervical spondylotic myelopathy. (2015)
  • Author(s): Morishita Y, et al.
  • Journal Title: Spinal Cord Volume: 53(5) Issue: 5 Pages: 408-12
  • DOI: 10.1038/sc.2014.137
  • Peer Reviewed
• [Journal Article] Effect of preservation of the C-6 spinous process and its paraspinal muscular attachment on the prevention of postoperative axial neck pain in C3-6 laminoplasty. (2015)
  • Author(s): Mori E, Ueta T, Maeda T, Yugue I, Kawano O, Shiba K.
  • Journal Title: J Neurosurg Spine. Volume: 22 Issue: 3 Pages: 221-9
  • DOI: 10.3171/2014.11.spine131153
  • Peer Reviewed
• [Journal Article] Radiological outcome of short segment posterior instrumentation and fusion for thoracolumbar burst fractures. (2015)
  • Author(s): Vu TT, Morishita Y, et al
  • Journal Title: Asian Spine J. Volume: 9(3) Issue: 3 Pages: 427-32
  • DOI: 10.4184/asj.2015.9.3.427
  • Peer Reviewed
• [Journal Article] Acute hyperglycemia impairs functional improvement after spinal cord injury in mice and humans (2014)
  • Author(s): Kobayakawa K, Kumamaru H, Saiwai H, Kubota K, Ohkawa Y, Kishimoto J, Yokota K, Ideta R, Shiba K, Tozaki-Saitoh H, Inoue K, Iwamoto Y, Okada S
  • Journal Title: Science Translational Medicine Volume: 6 Issue: 256 Pages: 256-256
  • DOI: 10.1126/scitranslmed.3009430
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathophysiological effects of lumbar instrumentation surgery on lumbosacral nerve roots in the vertebral foramen: measurement of local pressure of intervertebral foramen. (2014)
  • Author(s): Morishita Y, Maeda T, Ueta T, Naito M, Shiba K.
  • Journal Title: Spine. Volume: 39 Issue: 21 Pages: E1256-E1260
  • DOI: 10.1097/brs.0000000000000520
  • Peer Reviewed
• [Journal Article] Effect of Modic changes on spinal canal stenosis and segmental motion in cervical spine. (2014)
  • Author(s): Hayashi T, Daubs MD, Suzuki A, Phan K, Shiba K, Wang JC.
  • Journal Title: ur Spine J. Volume: 23 Issue: 8 Pages: 1737-42
  • DOI: 10.1007/s00586-014-3406-8
  • Peer Reviewed
• [Journal Article] Risk factors for missed dynamic canal stenosis in the cervical spine. (2013)
  • Author(s): Hayashi T, Wang JC, Suzuki A, Takahashi S, Scott TP, Phan K, Lord EL, Ruangchainikom M, Shiba K, Daubs MD.
  • Journal Title: Spine Volume: 39 Pages: 812-819
  • Peer Reviewed
• [Journal Article] Disturbance of rib cage development causes progressive thoracic scoliosis: the creation of a nonsurgical structural scoliosis model in mice. (2013)
  • Author(s): Kubota K, Doi T, Murata M, Kobayakawa K, Matsumoto Y, Harimaya K, Shiba K, Hashizume M, Iwamoto Y, Okada S.
  • Journal Title: J Bone Joint Surg Am. Volume: 95
  • Peer Reviewed
• [Journal Article] Therapeutic activities of engrafted neural stem/precursor cells are not dormant in the chronically injured spinal cord. (2013)
  • Author(s): Kumamaru H, Saiwai H, Kubota K, Kobayakawa K, Yokota K, Ohkawa Y, Shiba K, Iwamoto Y, Okada S.
  • Journal Title: Stem Cells. Volume: 31
  • Peer Reviewed
• [Presentation] 頚髄損傷患者における嚥下障害の危険因子 (2015)
  • Author(s): 林 哲生、坂井 宏旭、出田 良輔、植田 尊善、前田 健、芝 啓一郎
  • Organizer: 第50回日本脊髄障害医学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-11-19
• [Presentation] 受傷後 72 時間以内に診断された重篤麻痺を伴う 非骨傷性頚髄損傷に対する保存的治療経過 (2014)
  • Author(s): 森英治 、植田尊善 、前田健 、弓削 至 、河野修 、芝啓一郎
  • Organizer: 第49回日本脊髄障害医学会
  • Place of Presentation: 北海道旭川市
  • Year and Date: 2014-09-11 – 2014-09-12
• [Presentation] Facet joint angleが非骨傷性頚髄損傷の麻痺重症度に与える影響 (2013)
  • Author(s): 高尾恒彰、久保田健介、岡田誠司、森下雄一郎、前田健、植田尊善、林哲生、芝啓一郎
  • Organizer: 第42回日本脊椎脊髄病学会
  • Place of Presentation: 沖縄県宜野湾市