| Project/Area Number |
25670672
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology
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| Research Institution | Kobe University |
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Principal Investigator |
UEKI Masaaki 神戸大学, 医学(系)研究科(研究院), 研究員 (20213332)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 低酸素症 / イソフルラン / 脳神経発達 / エリスロポイエチン / 吸入麻酔薬 / 脳発達障害 |
|---|
| Research Abstract |
Erythropoietin (EPO), originally identified as a hematopoietic growth factor produced, is endogenously expressed in the central nervous system (CNS). EPO in the CNS is induced under hypoxic conditions and plays a role in neuroprotection. Mice were exposed to 8 % oxygen with or without 0.5 % isoflurane on postnatal 7day. Expression of EPO mRNA and caspase-3 mRNA and BDNF mRNA in brain was studied. Hypoxia-induced EPO mRNA expression in the brain was significantly suppressed and BDNF mRNA expression was induced by 0.5 % isoflurane. Mice after 8 % oxygen with 0.5 % isoflurane exposure had a greater learning impairment. Subcutaneous injection of EPO (5000 unit/kg) before 8 % oxygen with 0.5 % isoflurane exposure improved spatial memory/learning.
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