Analysis of cytoskeleton related genes in elderly patients

• Project/Area Number: 25670673
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: Sapporo Medical University
• Principal Investigator: Yamakage Michiaki 札幌医科大学, 医学部, 教授 (70285005)
• Co-Investigator(Kenkyū-buntansha): HAYASE TOMO 札幌医科大学, 医学部, 助教 (20579007) ; 杉野 繁一 東北大学, 大学病院, 助教 (00423765)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 術後認知機能障害 / 麻酔科学

Outline of Final Research Achievements

We investigated whether volatile anesthetics caused postoperative cognitive dysfunction (POCD). In humans, desflurane anesthesia showed a better mini mental examination score than did sevoflurane anesthesia. However, we could not find any genetic polymorphisms associated with cognitive function in humans. To examine the association between general anesthesia and POCD, we investigated whole mRNA expression in mouse hippocampal cells by a transcriptome-wide association study. While expression of the Lhx9 gene was suppressed by exposure to volatile anesthetics in juvenile mice, expression of Lhx9 gene was greatly enhanced by exposure to volatile anesthetics in adult mice. These results suggest that volatile anesthetics affect the differentiation of stem cells in the mouse hippocampus, and the results should be useful for exploring biomarkers of POCD.

Research Products

• [Journal Article] Effect of sevoflurane anesthesia on the comprehensive mRNA expression profile of the mouse hippocampus (2016)
  • Author(s): Hayase T, Tachibana S, Yamakage M.
  • Journal Title: Med Gas Res Volume: 6 Pages: 1-7
  • Peer Reviewed / Open Access
• [Journal Article] Nitrite Reduces Ischemia-Induced Ventricular Arrhythmias by Attenuating Connexin 43 Dephosphorylation in Rats. (2016)
  • Author(s): Maruyama D, Hirata N, Tokinaga Y, Kawaguchi R, Yamakage M.
  • Journal Title: Anesth Analg Volume: 122 Issue: 2 Pages: 410-417
  • DOI: 10.1213/ane.0000000000001063
  • Peer Reviewed
• [Journal Article] TACR1 gene polymorphism is associated with gender differences in postoperative nausea and vomiting. (2015)
  • Author(s): Hayase T, Sugino S, Moriya H, Yamakage M.
  • Journal Title: Anaesthesia Volume: 70 Issue: 10 Pages: 1148-1159
  • DOI: 10.1111/anae.13082
  • Peer Reviewed
• [Journal Article] Recovery of postoperative cognitive function in elderly patients after a long duration of desflurane anesthesia: a pilot study. (2015)
  • Author(s): Tachibana S, Hayase T, Osuda M, Kazuma S, Yamakage M.
  • Journal Title: J Anesth Volume: epub Issue: 4 Pages: 627-630
  • DOI: 10.1007/s00540-015-1979-y
  • Peer Reviewed
• [Journal Article] Efficacy and Safety of a Lidocaine and Ropivacaine Mixture for Scalp Nerve Block and Local Infiltration Anesthesia in Patients Undergoing Awake Craniotomy. (2014)
  • Author(s): Chaki T, Sugino S, Janicki PK, Ishioka Y, Hatakeyama Y, Hayase T, Kaneuchi-Yamashita M, Kohri N, Yamakage M.
  • Journal Title: J Neurosurg Anesthesiol Volume: epub Issue: 1 Pages: 1-5
  • DOI: 10.1097/ana.0000000000000149
  • Peer Reviewed
• [Journal Article] Single-nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects. (2014)
  • Author(s): Moriya H, Saito K, Helsby N, Hayashi N, Sugino S, Yamakage M, Sawaguchi T, Takasaki M, Takahashi M, Kurosawa N.
  • Journal Title: Biomed Rep. Volume: 2 Issue: 2 Pages: 265-269
  • DOI: 10.3892/br.2013.210
  • Peer Reviewed
• [Journal Article] Effects of local infiltration analgesia for posterior knee pain after total knee arthroplasty: comparison with sciatic nerve block. (2014)
  • Author(s): Gi E, Yamauchi M, Yamakage M, Kikuchi C, Shimizu H, Okada Y, Kawamura S, Suzuki T.
  • Journal Title: J Anesth Volume: Epub ahead of print Issue: 5 Pages: 696-701
  • DOI: 10.1007/s00540-014-1793-y
  • Peer Reviewed / Open Access
• [Journal Article] Phosphodiesterase 4 inhibitor roflumilast improves the bronchodilative effect of sevoflurane in sensitized airways. (2014)
  • Author(s): Zhou J, Iwasaki S, Yamakage M.
  • Journal Title: Anesthesiology Volume: 120 Issue: 5 Pages: 1152-1159
  • DOI: 10.1097/aln.0000000000000160
  • Peer Reviewed
• [Journal Article] Treatment of life-threatening hypercapnia with isoflurane in an infant with status asthmaticus. (2013)
  • Author(s): Masuda Y, Tatsumi H, Goto K, Imaizumi H, Yoshida SI, Kimijima T, Yamakage M.
  • Journal Title: J Anesth Volume: Epub ahead of print Issue: 4 Pages: 610-612
  • DOI: 10.1007/s00540-013-1751-0
  • Peer Reviewed
• [Journal Article] Substrate-dependent modulation of oxidative phosphorylation in isolated mitochondria following in vitro hypoxia and reoxygenation injury. (2013)
  • Author(s): Maruyama D, Hirata N, Miyashita R, Kawaguchi R, Yamakage M.
  • Journal Title: Exp Clin Cardiol Volume: 18 Pages: 158-160
  • Peer Reviewed
• [Presentation] Possible Mechanism of Neuroinflammation Induced by Surgical Procedure in the Mouse Hippocampus Determined by Using Transcriptome Analysis. (2015)
  • Author(s): Tachibana S, Hayase T, Yamakage M.
  • Organizer: The Annual Meeting of American Society of Anesthesiologists
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Prediction of Molecular Mechanism of Postoperative Nausea and Vomiting Using Transcriptome Analysis. (2015)
  • Author(s): Hayase T, Tachibana S, Yamakage M.
  • Organizer: The Annual Meeting of American Society of Anesthesiologists
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Effects of Propofol and Sevoflurane on Heating-induced Tension Generation of Vascular Smooth Muscle in Rats. (2015)
  • Author(s): Kimizuka M, Takada Y, Tokinlaga Y, Hayashi S, Kazuma S, Azumaguchi R, Yamakage M.
  • Organizer: The Annual Meeting of American Society of Anesthesiologists
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Elevation and Suppression of Paraoxon-induced Epileptic Activity in the Rat Hippocampus. (2015)
  • Author(s): Takada Y, Narimatsu E, Takahashi K, Niiya T, Yamakage M.
  • Organizer: The Annual Meeting of American Society of Anesthesiologists
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Cardioprotective Effect of Dexmedetomidine Against Ischemia/Reperfusion Injury in Hypertensive Hypertrophied Myocardium. (2015)
  • Author(s): Yoshikawa Y, Hirata N, Kawaguchi R, Miyashita R, Yamakage M.
  • Organizer: The Annual Meeting of American Society of Anesthesiologists
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Desflurane Preserves Blood Pressure During Anesthetic Induction Better Than Does Aevoflurane Due to Differential Effects on Autonomic Nervous System Evaluated by Heart Rate Variability (2014)
  • Author(s): Tachibana S, Maruyama D. Hirata N, Yamakage M.
  • Organizer: The annual meeting of the American Society of Anesthesiologistsal
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2014-10-11 – 2014-10-15
• [Presentation] Phosphodiesterase 4 Inhibitor Roflumilast Improves the Bronchodilative Effect of Sevoflurane in Sensitized Airway (2013)
  • Author(s): Jing Zhou, M.D., Sohshi Iwasaki, M.D.,Ph.D., Michiaki Yamakage, M.D.,Ph.D.
  • Organizer: American Society of Anesthesiologists Annual Meeting 2013
  • Place of Presentation: San Francisco, U.S.A
• [Presentation] Oprm1 Gene Polymorphism Reduces DNA Methylation at the Upstream CpG Island in Male Patients. (2013)
  • Author(s): Shigekazu Sugino, M.D.,Ph.D., Tomo Hayase, M.D., Misako Higuchi, M.D., Yuko Nawa, M.D.,Ph.D., Yukihiro Kumeta, M.D.,Ph.D., Michiaki Yamakage, M.D., Ph.D.
  • Organizer: American Society of Anesthesiologists Annual Meeting 2013
  • Place of Presentation: San Francisco, U.S.A
• [Presentation] Recovery of Postoperative Cognitive Function in Elderly Patients After a Long Duration of Desflurane Anesthesia. (2013)
  • Author(s): Satoshi Kazuma, M.D., Tomo Hayase, M.D., Michiko Osuda, M.D., Shigekazu Sugino, M.D.,Ph.D., Michiaki Yamakage, M.D.,Ph.D.
  • Organizer: American Society of Anesthesiologists Annual Meeting 2013
  • Place of Presentation: San Francisco, U.S.A
• [Presentation] Bone Marrow-Derived Microglia That Express IL-1β Might Cause Negative Emotions Induced by Neuropathic Pain (2013)
  • Author(s): Atsushi Sawada, M.D., Yukitoshi Niiyama, M.D.,Ph.D., Michiaki Yamakage, M.D.,Ph.D.
  • Organizer: American Society of Anesthesiologists Annual Meeting 2013
  • Place of Presentation: San Francisco, U.S.A
• [Presentation] Mechanism of the Inhibitory Effect of Desflurane on Angiotensin II-Induced Vasoconstriction. (2013)
  • Author(s): Yasuyuki Tokinaga, M.D.,Ph.D., Yukimasa Takada, M.D., Shunsuke Hayashi, No Degree, Masayuki Akatsuka, M.D., Michiaki Yamakage, M.D., Ph.D.
  • Organizer: American Society of Anesthesiologists Annual Meeting 2013
  • Place of Presentation: San Francisco, U.S.A
• [Book] 必携麻酔科初期研修マニュアル―3カ月麻酔科研修を充実したものとするために! (2014)
  • Author(s): 山蔭 道明、枝長 充隆
  • Total Pages: 349
  • Publisher: 真興交易医書