Induction and isolation of renal stem cells and establishment of foundation for renal regeneration research.

• Project/Area Number: 25670683
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: Watanabe Masami 岡山大学, 大学病院, 教授 (70444677)
• Co-Investigator(Kenkyū-buntansha): NOGUCHI HIROFUMI 琉球大学, 医学部, 教授 (50378733) ; SUGIMOTO MORITO 岡山大学, 岡山大学病院, 助教 (30613161) ; UEKI HIDEO 岡山大学, 医学部, 技術専門職員 (90537218) ; KAKU HARUKI 岡山大学, 岡山大学病院, 講師 (60346426)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 再生医学 / 腎臓 / 再生 / 幹細胞

Outline of Final Research Achievements

We herein aimed to establish human renal stem cells for renal regeneration research, without using iPS and ES cells. We established several clones of renal stem cells derived from primary proximal tubular cells by transfecting iPS four factors. The tumorigenicity was not observed in the renal stem cells when subcutaneously inoculated into nude mice. We also established clones of renal stem cells derived from the glomerular endothelial cells, and confirmed VE-CADHERIN expression in the renal stem cells.