Development of visible light inducible photocatalytic nano-particles for the diagnosis of bladder cancer

• Project/Area Number: 25670684
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Yokohama City University
• Principal Investigator: KUBOTA Yoshinobu 横浜市立大学, 医学(系)研究科(研究院), 教授 (10106312)
• Co-Investigator(Kenkyū-buntansha): YAO Masahiro 横浜市立大学, 医学（系）研究科（研究院）, 教授 (10106312) ; 矢尾 正祐 横浜市立大学, 医学(系)研究科(研究院), 教授 (00260787)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膀胱癌 / 光力学治療 / 光触媒 / ヘマトポルフィリン / 可視光応答型光触媒

Outline of Final Research Achievements

In this study, we tried to construct a novel nano-composite which consisted of visible light sensitive photocatalyst and Hematoporphyrin (Hp), and apply it to in situ diagnosis of bladder carcinoma.As results 1. We could successfully make Polyethylene glycol (PEG) modified TiO2 nanoparticles (PEG-TiO2) and a complex of TiO2/PEG and Hp (PEG-TiO2-Hp nanoparticles).2.The uptaken to human bladder cancer cells of these PEG-TiO2-Hp were confirmed and its fluorescence were around cytoplasm of the cells. The florescence intensity was stronger than when using Hp only. 3. The fluorescence intensity of treated cells were depended on treatment time and concentration of PEG-TiO2-Hp. These results suggested that PEG-TiO2-Hp could be potentially useful for apply to in situ diagnosis of bladder carcinoma.As future plan we will improve these compounds and will perform the toxic study of the conjugates for the application to human.

Research Products

• [Journal Article] Discolored Ureteral Stents：Findings in Urinalysis and Urine Culture (2015)
  • Author(s): Takashi Kawahara, Hiroshi Mlyamoto, Hiroki Ito, Hideyuki Terao,Hroji Uemura,Yoshinobu Kubota,Junichi Matsuzaki
  • Journal Title: PLOS ONE Volume: 10 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Photocatalytic activity of Cu2＋/TiO2-coated cordierite foam inactivates bacteriophages and LEgionella pneumophila (2013)
  • Author(s): Hitoshi Isiguro,Yanyan YAo,Ryuichi Nakano, Masayuki Hara,Kayano Sunada,Kazuhito Hashimoto,Jitsuo Kajioka, Akira Fujisima,Yosinobu Kubota
  • Journal Title: Applied Catalysis B:Environmental Volume: 129 Pages: 56-61
  • Peer Reviewed
• [Journal Article] Broad Spectrum Microbicidal Activity of Photocatalysis by TiO2 (2013)
  • Author(s): Ryuichi Nakano,Masayuki Hara,Hitoshi Ishiguro, Yanyan Yao, Tsuyoshi Ochiai,Kazuya Nakata,Taketoshi Murakami,Jitsuo Kajioka,Kayano Sunada,Kazuhito Hashimoto,Akira Fujishima,Yoshinobu Kubota
  • Journal Title: Catalysts Volume: 3 Pages: 310-323
  • Peer Reviewed
• [Presentation] Test methods for antiviral activity of photocatalytic TiO2: ISO Protocol (2014)
  • Author(s): Yoshinobu Kubota
  • Organizer: SIEMME20
  • Place of Presentation: 成都市(中華人民共和国）
  • Year and Date: 2014-09-22
• [Presentation] Study on antiviral activity of photocatalytic TiO2 (IV)
  • Author(s): Yoshinobu Kubota,Hitoshi Isiguro,Ryuichi Nakano,Masayuki Hara,Jitsuo Kajioka,Akira Fujishima
  • Organizer: SIEMME19
  • Place of Presentation: 中華人民共和国北京市
  • Invited