| Project/Area Number |
25670687
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
MIKI Tsuneharu 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10243239)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIMURA Yasunori 京都府立医科大学, 医学研究科, 助教 (20398374)
KAMOI Kazumi 京都府立医科大学, 医学研究科, 講師 (40295663)
UEDA Takashi 京都府立医科大学, 医学研究科, 助教 (50601598)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | MRTF-A分子 / 前立腺癌治療 / 細胞骨格 / 癌転移抑制 / MRTF分子 |
|---|
| Outline of Final Research Achievements |
We identified that MRTF-A inhibits cancer cell motility by upregulating the various kinds of actin binding proteins in vitro. It was indicated that MRTF-A might be related with tumor-suppressor gene p53 according to the data of MRTF-A promoter assay and knockdown of p53 gene using siRNA in prostate cancer cells. We made adenovirus and retrovirus vectors which overexpress MRTF-A towards prostate cancer cells. Overexpression of MRTF-A in prostate cancer cells reduced cancer cell invasion in culture. Analysis of the effect for tumor cell metastasis in vivo in a mouse model of prostate cancer using these vectors is currently in progress.
|
