| Project/Area Number |
25670691
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
ARIMA TAKAHIRO 東北大学, 医学(系)研究科(研究院), 教授 (80253532)
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| Co-Investigator(Kenkyū-buntansha) |
OKAE Hiroaki 東北大学, 医学(系)研究科(研究院), 助教 (10582695)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 胎盤 / エピジェネティクス / ゲノムインプリンティング / エピジェネティックス |
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| Outline of Final Research Achievements |
Genomic imprinting is an epigenetic gene regulatory mechanism which leads to the preferential monoallelic expression of a subset of genes. The majority of imprinted genes has differntial methylated regions (DMRs). While the importance of DNA methylation is widely accepted, the presence of DNA methylation-independent establishment of imprinting is also proposed for some imprinted genes (Okae et al., 2012). Somatic cell nuclear transfer (SCNT) can produce viable individuals, but the success rate is very low and many abnormalities were reported for SCNT-derived animals. In this study, we performed a transcriptome-wide analysis of imprinted gene expression in cloned mouse placenta and found that several imprinted genes did in fact show LOI. Our data advances the understanding the underlying mechanisms of reprogramming of somatic cell nuclei and the regulatory mechanisms of genomic imprinting.
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