Analysis of natural history and the neoplastic nature of lobular endocervical glandular hyperplasia (LEGH)

• Project/Area Number: 25670699
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Shinshu University
• Principal Investigator: MIYAMOTO Tsutomu 信州大学, 学術研究院医学系(医学部附属病院), 講師 (70418721)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Kaori 信州大学, 医学部附属病院, 医員 (70646653) ; TACHIBANA Ryota 信州大学, 学術研究院医学系(医学部附属病院), 助教 (90623687) ; SHIOZAWA Tanri 信州大学, 学術研究院医学系, 教授 (20235493)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 悪性腺腫 / 分葉状頸管腺過形成 / 臨床診断 / 臨床経過 / 細胞診 / 胃型粘液 / MRI / GNAS / クロナリティー解析

Outline of Final Research Achievements

To explore the natural history of lobular endocervical glandular hyperplasia (LEGH), the patients with cervical multi-cystic lesion (CMCL) were managed by the clinical diagnostic method combined with endocervical cytology, MRI and detection of gastric type mucin. In total 109 cases, 7 cases were cervical adenocarcinoma including 1 case clinically diagnosed as suspicious of LEGH (LEGH S/O) (85.7% sensitivity). Forty-one cases of LEGH S/O and 26 cases of retention cysts (NC) were followed-up for more than 12 months, and no one was developing to carcinoma. These results suggest our clinical diagnostic methods may be useful for the detection of malignant cases and for follow-up of LEGH cases. Next, GNAS gene mutation was investigated using laser-microdissection and direct sequence in 10 cases of LEGH, 5 cases of atypical LEGH and 2 cases of adenoma malignum (MDA), and detected in 2 cases of atypical LEGH. These results suggest GNAS mutation may be involved in the malignant change of LEGH.

Research Products

• [Journal Article] 4-hydroxy estrogen induces DNA damage on codon 130/131 of PTEN in endometrial carcinoma cells (2015)
  • Author(s): Ke H, Suzuki A, Miyamoto T, Kashima H, Shiozawa T
  • Journal Title: Mol Cell Endocrinol Volume: 400 Pages: 71-7
  • DOI: 10.1016/j.mce.2014.10.027
  • Peer Reviewed
• [Journal Article] Recurrent low-grade endometrial stromal sarcoma with intracaval and intracardiac tumor thrombus: diagnosis, treatment, and surgical management (2014)
  • Author(s): Miyamoto T, Tachibana R, Kobara H, Takano T, Kato H, Shimizu A, Ohya A, Uehara T, Shiozawa T.
  • Journal Title: International Cancer Conference Journal Volume: 3 Issue: 1 Pages: 1-7
  • DOI: 10.1007/s13691-013-0134-6
  • Peer Reviewed
• [Journal Article] Morphological alterations in protamine-deficient spermatozoa (2014)
  • Author(s): Utsuno H, Miyamoto T, Oka K, Shiozawa T
  • Journal Title: Hum Reprod Volume: 29 Issue: 11 Pages: 2374-81
  • DOI: 10.1093/humrep/deu225
  • Peer Reviewed
• [Journal Article] Polypoid endometriosis of the ovary mimicking ovarian carcinoma dissemination: a case report and literature review (2014)
  • Author(s): 4.Yamada Y, Miyamoto T, Horiuchi A, Ohya A, Shiozawa T
  • Journal Title: J Obstet Gynaecol Res Volume: 40 Issue: 5 Pages: 1426-30
  • DOI: 10.1111/jog.12358
  • Peer Reviewed
• [Journal Article] Clonality analysis suggests that STK11 gene mutations are involved in progression of lobular endocervical glandular hyperplasia (LEGH) to minimal deviation adenocarcinoma (MDA). (2013)
  • Author(s): Takatsu A, Miyamoto T, Fuseya C, Suzuki A, Kashima H, Horiuchi A, Ishii K, Shiozawa T
  • Journal Title: Virchows Arch Volume: 462(6) Issue: 6 Pages: 645-51
  • DOI: 10.1007/s00428-013-1417-1
  • Peer Reviewed
• [Presentation] 1.子宮頸部嚢胞性病変の取り扱い2.子宮内膜癌の発生とエストロゲン
  • Author(s): 塩沢丹里
  • Organizer: 第21回鴨和腫瘍カンファレンス
  • Place of Presentation: 京都