| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Outline of Final Research Achievements |
Spatiotemporal expression of transcription factors is crucial for genomic reprogramming processes in both in vivo and in vitro. Cellular reprogramming and differentiation are accompanied by dynamic transcriptional changes and transcription factors play central roles in both processes. Dramatic alterations of cellular fates occur during preimplantation embryo development. In mice, after fertilization, an important transcription factor responsible for sustaining pluripotency is the Pou5f1 protein (also known as Oct4). Our deep expression analysis and dose-dependent functional analyses in the preimplantation stages were conducted to address the in vivo Oct4 potential for developmental reprogramming. Our recent findings provide new insight into a role for Oct4 in epigenetic reprogramming toward totipotency in vivo. The spatiotemporal regulation and fine tuning of Oct4 is essential for cellular reprogramming and integrity.
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