| Project/Area Number |
25670711
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Tomoo 山形大学, 医学部, 講師 (60344793)
ITO Tsukasa 山形大学, 医学部, 助教 (50344809)
FURUKAWA Takatoshi 山形大学, 医学部, 非常勤講師 (80466630)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 顔面神経麻痺 / ヒト皮膚線維芽細胞 / 機能再生 / ラット / 顔面神経損傷モデル / ヒト皮膚繊維芽細胞 / 神経再生 / Matrigel |
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| Outline of Final Research Achievements |
Objective: To determine the effect of transplanting normal human dermal fibroblasts (NHDF) in the rat facial nerve palsy model. Methods: Eight weeks old Wister male rats (250g) were used. Facial nerves were transected at buccal branch and marginal branch in 6 mm length under general anesthesia. Polimer tubes filled with NHDF plus matrigel were grafted between the cut nerves. Wisking amplitude was measured to determine the facial nerve function. Result: Wisking amplitude was gradually recovered in 8 week. However, the effect of NHDF was not significant.
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