| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Autophagy can help cancer cells to overcome metabolic stress and the cytotoxicity of chemotherapy.Beclin1 has been well-characterized to play a pivotal role in autophagy. Cisplatin (CDDP) is central to the treatment of nasopharyngeal carcinoma (NPC). Autophagy may contribute to acquire resistance to chemotherapeutic agents, but it is unclear that inhibition of autophagy promotes death of cancer cells or tumor growth. In this study, we examined the role of autophagy in NPC and Beclin1 was used as an index. Immunohistochemical analysis is performed to evaluate autophagy in NPC tissue.Beclin1 protein levels in NPC cell lines with CDDP and/or CQ were evaluated with Western blot analysis. We also examined the viability of NPC cell lines with CDDP and/or CQ.
Beclin1 expression is significantly higher after chemotherapy than before. In vitro concomitant therapy (CDDP and CQ) was more effective in restraining NPC cells proliferation.
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